13C NMR spectra of plant pathogenic fungi.

Abstract

13C NMR spectra of intact mycelia of Pyricularia oryzae, Botrytis cinerea, and five species of Pythium were measured. Sharp signals assigned to trehalose, mannitol and triglycerides in the cytoplasm were observed in the spectra of P. oryzae and B. cinerea. (l->3)-/?-Glucan and triglycerides were detected in all species of Pythium examined, and trehalose was observed in P. intermedium, P. ultimum and P. spinosum. In addition, glucose was detected in the latter two species. No remarkable signals from proteins, nucleic acids, membranelipids or cell wall polysaccharides wereobserved. Recently NMRspectroscopy has been applied to the fields of biochemistry, physiology and medicine. One well-known application is 31P NMRof intact cells and organs.1* Energy metabolism in living cells can be observed by following changes in the 31P NMRspectra due to ATP, ADPand sugar phosphates. It is possible to estimate the pHof the matrix of the cytoplasm or organelles by examining the chemical shift of inorganic phosphate. Various samples, microorganisms, cultured cells and

Metabolomics Protocols for Filamentous Fungi

“…reference for the chemical shifts of resonance peaks. These include acetone ( 26 ) , acetate ( 27 ) , EDTA (28)(29)(30) , dioxane ( 31,32 ) , hexamethyldisiloxane ( 33 ) , TMS ( 29,(34)(35)(36)(37)(38) and trimethylsilyl propionate (TMSP) ( 39 ) . Defi ciencies have been noted with each of these and the recommendation has been made that 2,2-dimethyl-2-silapentane-5-sulfonic acid (DSS) be used as the universal standard, due to its being insensitive to variations in temperature and pH, and the fact that it is chemically inert and has a single, sharp, unambiguous highfi eld resonance peak ( 40 ) .…”