14-3-3 proteins: potential roles in vesicular transport and Ras signaling in Saccharomyces cerevisiae.

Gelperin, Daniel; Weigle, Jeffrey; Nelson, Karen K.; Roseboom, Patrick H.; Irie, Kenji; Matsumoto, Kunihiro; Lemmon, Sandra K.

doi:10.1073/pnas.92.25.11539

Cited by 155 publications

(162 citation statements)

References 34 publications

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“…A null mutation of CHC1 is lethal in certain strains and leads to slow growth, morphological and membrane trafficking defects in other strains. 17 Only a genetic interaction between CHC1 and BMH1 and BMH2 was demonstrated and there is no evidence for physical interactions. Deletion of the C-terminal region abolishes the ability of 14-3-3 to suppress a lethal chc1 mutation.…”

Section: Exocytosis and Vesicle Transportmentioning

confidence: 99%

“…17 Clathrin is a vesicular coat protein involved in receptor-mediated endocytosis and protein sorting at the late Golgi compartment. A null mutation of CHC1 is lethal in certain strains and leads to slow growth, morphological and membrane trafficking defects in other strains.…”

Section: Exocytosis and Vesicle Transportmentioning

confidence: 99%

“…Deletion of the C-terminal region abolishes the ability of 14-3-3 to suppress a lethal chc1 mutation. 17 It is likely that the C-termini of the yeast isoforms, which are longer compared to mammalian isoforms and contain a glutamine stretch, are involved in regulation of vesicle transport. The 14-3-3 proteins are involved in exocytosis in mammalian cells through rearrangement of the actin cytoskeleton.…”

Section: Exocytosis and Vesicle Transportmentioning

confidence: 99%

“…Overexpression of the BMH genes suppresses mutations in CDC25 and TPK1. 17 This suggests that the yeast 14-3-3 proteins participate in the Ras/PKA signalling pathway. In addition, BMH1 is required for Ras-dependent activation of human Raf-1 in a reconstituted yeast system, through direct physical interaction with Raf-1.…”

Section: Exocytosis and Vesicle Transportmentioning

confidence: 99%

“…The two S. cerevisiae 14-3-3 proteins, encoded by BMH1 and BMH2, 17,43,45 are more than 60% identical to the mammalian e isoform. Bmh1p and Bmh2p are 91% identical and 97% identical, respectively, over the first 250 residues, therefore any isoform-specific function probably resides in the divergent C-termini.…”

Section: Introductionmentioning

confidence: 99%

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Yeast 14‐3‐3 proteins

Hemert

Heusden

Steensma

2001

Yeast

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Section: Exocytosis and Vesicle Transportmentioning

confidence: 99%

Section: Exocytosis and Vesicle Transportmentioning

confidence: 99%

Section: Exocytosis and Vesicle Transportmentioning

confidence: 99%

Section: Exocytosis and Vesicle Transportmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Yeast 14‐3‐3 proteins

Hemert

Heusden

Steensma

2001

Yeast

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Systematic screening of the 14-3-3 eta (?) chain gene for polymorphic variants and case-control analysis in schizophrenia

Bell¹,

Munro²,

Russ³

et al. 2000

Am. J. Med. Genet.

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The neuronal protein 14-3-3 eta is a candidate gene for schizophrenia because it maps chromosome 22q12, a region implicated in the disease by linkage analysis, and is involved in brain development. We systematically screened this gene for polymorphic variants by comparison of public EST sequence data (five cDNAs and 72 ESTs, 21,155 bp of sequence) in parallel with single-stranded conformational polymorphism analysis, and we compared these methods by using a simple power calculation. Twelve potential polymorphisms were identified from EST sequence comparison, and two of these (a 5'-VNTR and 753G/A) were confirmed by SSCP analysis and sequencing. Three additional infrequent polymorphisms (-408T/G; 177 C/G; and 989 A/G) were found by SSCP only. We next examined these variants for association with schizophrenia. One variant in untranslated region of exon 1 (-408 T/G) was found to occur more frequently in the schizophrenic subjects (8%) than the controls (3%; P = 0.01). After fivefold correction of the P value for multiple testing, marginal association was found. Haplotype analysis of pairs of polymorphisms provided no evidence for association of this gene with schizophrenia in the population studied. Am. J. Med. Genet. (Neuropsychiatr. Genet. ) 96:736-743, 2000.

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A proteomic approach for the study ofSaccharomyces cerevisiae cell wall biogenesis

Pardo

Ward²,

Bains³

et al. 2000

Electrophoresis

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In fungi, cell shape is determined by the presence of a rigid cell wall which separates the cell from the extracellular medium. This highly dynamic structure is essential for the maintenance of cell integrity and is involved in several phenomena such as flocculation, adherence and pathogenicity. The composition of the fungal cell wall is well known, but issues such as the assembly and remodeling of its components remain poorly understood. In an attempt to study the de novo construction of the yeast cell wall, we have undertaken a large-scale proteomic approach to analyze the proteins secreted by regenerating protoplasts. Upon incubation of protoplasts in regenerating conditions, numerous proteins are secreted into the culture medium. These presumably include proteins destined for the cell wall, comprising both structural proteins as well as enzymes involved in cell wall biogenesis. This work reports the establishment of a reference map of proteins secreted by regenerating protoplasts by means of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and their identification by mass spectrometry. Thirty-two different proteins have been identified, including known cell wall proteins, glycolytic enzymes, heat shock proteins, and proteins involved in several other processes. Using this approach, novel proteins possibly involved in cell wall construction have also been identified. This reference map will allow comparative analyses to be carried out on a selected collection of mutants affected in the cell wall.

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14-3-3 proteins: potential roles in vesicular transport and Ras signaling in Saccharomyces cerevisiae.

Abstract: Deletion of the clathrin heavy-chain gene,

Cited by 155 publications

References 34 publications

Yeast 14‐3‐3 proteins

Yeast 14‐3‐3 proteins

Systematic screening of the 14-3-3 eta (?) chain gene for polymorphic variants and case-control analysis in schizophrenia

A proteomic approach for the study ofSaccharomyces cerevisiae cell wall biogenesis

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