14-3-3σ Protein Expression in Canine Renal Cell Carcinomas

Abstract

14-3-3σ is a protein expressed in many epithelial tissues associated with essential cell functions, including cell-cycle control, apoptosis, and cytoskeletal integrity. There is a paucity of knowledge of the tumorigenesis of canine renal cell carcinomas (RCCs), and the histological origin of this tumor has not been established. This study analyzed the expression of 14-3-3σ, Ki-67, cytokeratins, and vimentin in 40 canine RCCs. Aberrant expression of 14-3-3σ was demonstrated in 15 (38%) cases and was associated with a significantly shorter survival time ( P < .002). In contrast to canine RCC, normal kidney did not express 14-3-3σ. The Ki-67 proliferation index did not show utility as a prognostic factor. The distal convoluted tubular epithelium in normal kidneys coexpressed cytokeratins and vimentin, and thus maintenance of this coexpression pattern in canine RCC suggests that most tumors arise from the distal segment of the nephron. These results suggest that 14-3-3σ is a potential negative prognostic factor and a possible therapeutic target.

Expression profile and prognostic value of SFN in human ovarian cancer

“…In response to DNA damage, this protein plays a role in preventing DNA errors during mitosis. Previous studies indicated that SFN participated in multiple kinds of tumor, including lung [17], renal [18], liver [19], and breast [20,21] cancer. Especially for lung cancer, several studies demonstrated that SFN played as a novel oncogene, stimulating the tumor initiation, and progression of lung adenocarcinoma [8,17].…”

Molecular carcinogenesis in equine penile cancer: A potential animal model for human penile cancer

“…In various human carcinomas, including those of the head and neck, cervical and vulvar carcinomas, altered 14-3-3σ expression is associated with greater invasiveness, proliferation and metastasis [15,17,35]. Furthermore, in canine inflammatory mammary carcinoma, a very aggressive type of carcinoma that resembles inflammatory breast carcinoma in women, and in canine renal cell carcinomas, there is a significant neo-expression of 14-3-3σ protein and it is an independent prognostic indicator of shorter survival time [36,37]. Expression of 14-3-3σ in tumoral cells is usually cytoplasmic, however in very aggressive neoplasms nuclear translocation can occur and is associated with cell proliferation.…”

Canine Gastric Carcinomas: A Histopathological and Immunohistochemical Study and Similarities with the Human Counterpart

“…However, acinar structures within the intravascular emboli lacked expression of 14-3-3σ. Aberrant nuclear expression of 14-3-3σ, in a subset of cases of renal cell carcinomas, was associated with a malignant phenotype and shorter survival rate [ 43 ]. Thus, we hypothesize, in view of these findings, that expression of 14-3-3σ is associated with features of malignancy, and that neoexpression of the molecule is essential in the stage of intravascular invasion.…”

“…14-3-3σ labeling was assessed using a previously published semiquantitative scoring system [ 43 ]. The scores for the percentage (1, ≤10% cells; 2, 11–50% cells; and 3, >50% cells) and staining intensity (0, negative; 1, weak; 2, moderate; and 3, intense) of positive cells were recorded and a TIS 14-3-3σ (ranging from 0 to 9) was calculated as the product of these two parameters for each of the studied cases.…”