140PD The role of leptin and its receptor in oligo-astrocytic neoplasms

Vokuda, Ramya S; Bh, Shapiro; Madhugiri, V.S.; Kumar, Sushil

doi:10.1093/annonc/mdw578.003

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“…The expression of leptin and its receptor was evaluated immunohistochemically, as previously described by us (Vokuda et al, 2017), and subsequently confirmed by western blotting. The following primary antibodies were used: Leptin (Ob-A-20) rabbit polyclonal antibody (1:50 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and Leptin Receptor (ObR-H-300) rabbit polyclonal antibody (1:50 dilution, Santa Cruz Biotechnology).…”

Section: Immnuohistochemistrymentioning

confidence: 99%

The Expression of Leptin and Its Receptor During Tumorigenesis of Diffuse Gliomas such as Astrocytoma and Oligodendroglioma - Grade II, III and IV (NOS)

Vokuda

Srinivas

Madhugiri

et al. 2019

Asian Pac J Cancer Prev

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Background:Leptin, an adipocytokine functions via the leptin receptor, OB-Rb that contains an intact intracellular domain and activates the JAK/STAT signalling cascade. It stimulates growth, migration and invasion of cancer cells in vitro potentiating angiogenesis. Recently, the involvement of leptin in tumor progression is being explored. Gliomas exhibit poor prognosis, low survival rates demanding for novel therapeutic regimens resulting in discovery of many potential biomarkers and pharmaceutical targets. We analysed the potential role of leptin and OB-Rb in carcinogenesis of malignant gliomas. Methods:Sixty fresh tissue samples of diffuse gliomas were collected after tumor excision. Real time PCR, immunohistochemical (IHC) analysis and western blot analysis were carried out to assess the expression of leptin and its receptor. Results:The present study demonstrates the expression of leptin and LepR and their involvement in tumor progression. Of the 60 cases, 57 cases (95%) and 53 cases (88.3%) showed amplification for leptin and OB-Rb respectively. The expression of these proteins were measured semi-quantitatively and correlated with degree of malignancy (p<0.05). The bands were visualised on western blot. Conclusion:Leptin may be valued as a pharmaceutical target and anti-leptin compounds could be developed as drugs in mono- or combined therapies for these tumors.

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Section: Immnuohistochemistrymentioning

confidence: 99%