146 Promising tools to facilitate the implementation of TDM of biologics in clinical practice

Soenen, Rani; Stove, C.; Capobianco, A.; Schutter, H. De; Dobbelaere, M.; Mahjor, T.; Follens, M.; Lambert, J.; Grine, L.

doi:10.1016/j.jid.2022.09.156

Cited by 1 publication

(1 citation statement)

References 32 publications

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“…In recent years, VAMS has become an effective tool and is used in several clinical applications, ranging from therapeutic drug monitoring [ 7 , 8 ] to proteomics [ 9 ] and metabolomics, as we recently described [ 10 ]. This microsampling technique foresees that blood is collected via a finger prick and becomes absorbed into a hydrophilic polymer [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

VAMS-Based Blood Capillary Sampling for Mass Spectrometry-Based Human Metabolomics Studies

Volani

Malfertheiner²,

Caprioli³

et al. 2023

Metabolites

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Volumetric absorptive microsampling (VAMS) is a recently developed sample collection method that enables single-drop blood collection in a minimally invasive manner. Blood biomolecules can then be extracted and processed for analysis using several analytical platforms. The integration of VAMS with conventional mass spectrometry (MS)-based metabolomics approaches is an attractive solution for human studies representing a less-invasive procedure compared to phlebotomy with the additional potential for remote sample collection. However, as we recently demonstrated, VAMS samples require long-term storage at −80 °C. This study investigated the stability of VAMS samples during short-term storage and compared the metabolome obtained from capillary blood collected from the fingertip to those of plasma and venous blood from 22 healthy volunteers. Our results suggest that the blood metabolome collected by VAMS samples is stable at room temperature only for up to 6 h requiring subsequent storage at −80 °C to avoid significant changes in the metabolome. We also demonstrated that capillary blood provides better coverage of the metabolome compared to plasma enabling the analysis of several intracellular metabolites presented in red blood cells. Finally, this work demonstrates that with the appropriate pre-analytical protocol capillary blood can be successfully used for untargeted metabolomics studies.

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