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Determination of gibberellins using HPLC coupled with fluorescence detection

“…Due to the ultra trace levels of GAs in plants (typically in the range of 10 À9 to 10 À15 mol g À1 ) and the complicated matrix of plant samples, the determination of GAs in plants needs efficient sample preparation and sensitive detection techniques. To date, a number of analytical techniques have been employed for the quantitative analysis of GAs, such as excitation-emission matrix uorescence, 7 capillary electrophoresis (CE), 8,9 high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection, 10,11 gas chromatography (GC) with mass spectrometry (MS), [12][13][14] LC-MS [15][16][17][18][19][20] and so on. Among these techniques, HPLC-MS is the most powerful one to analyze phytohormones including GAs because of its high sensitivity and excellent qualitative analysis ability.…”