Untitled

Ledneva, R. K.; Alexeevskii, A. V.; Васильев, С. А.; Spirin, Sergei A.; Karyagina, A. S.

doi:10.1023/a:1012309917322

Cited by 16 publications

(9 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ledneva et al have employed lignin sulphonates in the sulphuric acid carbonising liquor at concentrations of 3-5 gA, depending on the level of contamination of the fibre [49,50]. They claim that fibre strength loss is reduced from 15 to 4%.…”

Section: Acidising Formulationsmentioning

confidence: 99%

“…More recently Gething has reported that, since the end of the Korean War, the number of companies involved in scouring and carbonising in Australia had dropped from [45][46][47][48][49][50] to about 18 at the present time [6]. The volume of wool processed by those remaining mills has increased considerably and, for the 1988/ 89 year, was some 18.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Developments in wool carbonising

Pailthorpe¹

1991

Review of Progress in Coloration and Related Topics

View full text Add to dashboard Cite

Section: Acidising Formulationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Developments in wool carbonising

Pailthorpe¹

1991

Review of Progress in Coloration and Related Topics

View full text Add to dashboard Cite

“…On the other hand, major drawbacks of noncovalent assemblies are their lower uptake efficiencies and higher complexity for structure determination because these entities can form aggregates with very complex secondary and tertiary structures. ,, This last feature in particular represents a key issue either for establishing structure–activity relationships or for optimizing the production of new vectors intended for gene delivery. , In addition, the third helix at the C-terminus of homeoproteins is known to dock with DNA major grooves, and although several homeodomains have been identified to date, this region is highly conserved among different homeoproteins. For instance, certain amino acids are systematically found at certain positions: V or I at 45, W at 48, F at 49, and R or K at 52, 55, and 57. , The Penetratin sequence obeys this prototypical composition, and therefore, information on supramolecular ordering of Penetratin /DNA condensates represents a potential contribution to shed light on the regulatory function of Antennapedia .…”

Section: Introductionmentioning

confidence: 97%

“…For instance, certain amino acids are systematically found at certain positions: V or I at 45, W at 48, F at 49, R or K at 52, 55 and 57. 25,26 The Penetratin sequence obeys this prototypical composition and, therefore, information on supramolecular ordering of Penetratin/DNA condensates represents a potential contribution to shed light on the regulatory function of Antennapedia.…”

Section: Introductionmentioning

confidence: 99%

Nanoscopic Structure of Complexes Formed between DNA and the Cell-Penetrating Peptide Penetratin

et al. 2019

View full text Add to dashboard Cite

One of the most remarkable examples of cell-penetrating peptides (CPPs) is Penetratin, a 16-mer fragment derived from the Drosophila Antennapedia homeobox. Understanding the structure of Penetratin/DNA complexes is a key factor for the successful design of new vectors for gene delivery and may assist in optimizing molecular carriers based on CPPs. Herein, we present a comprehensive study on the nanoscale structure of noncovalent complexes formed between Penetratin and DNA. The strong cationic nature of the peptide makes it a very efficient agent for condensing DNA strands via electrostatic attraction and we show for the first time that condensation is accompanied by random-to--sheet transitions of peptide secondary structure, demonstrating that nucleic acids behave as a structuring agent upon complexation. For the first time, nanoscale-resolved spectroscopy is used to provide single-particle infrared data from DNA carriers based on CPPs and they show that the structures are stabilized by Penetratin -sheet cores, whereas larger DNA fractions are preferentially located in the periphery of aggregates. Insolution infrared assays indicate that phosphate diester groups are strongly affected upon DNA condensation, presumably as consequence of charge delocalization induced by the proximity of cationic amide groups in Penetratin. The morphology is characterized by nano-assemblies with surface fractal features and short-range order is found in the inner structure of the scaffolds. Interestingly, the formation of beads-on-a-string arrays is found, producing nanoscale architectures that resemble structures observed in early steps of chromatin condensation. A complexation pathway where DNA condensation and peptide pairing into sheets are key steps for organization is proposed.

show abstract

“…However, among the sequences found to be preferentially cleaved were the thymine and adenine including engrailed, NK-2, and Pbx1 some of which are known to adopt a canonical extended recognition helix (α3) or additional C-terminal helices only upon binding their target DNA. 51,[143][144][145][146] EuP3W, unlike EuP5b, has super secondary structure independent of DNA binding, although this structure also appears to be enhanced and stabilized by specific nucleic acid interactions. A pre-organized structure could potentially approach the DNA groove in several ways.…”

Section: Resultsmentioning

confidence: 99%

DNA sequence selectivity and kinetic properties of de novo designed metalloprotein dimers

Wong-Deyrup¹

View full text Add to dashboard Cite

In our efforts to engineer a DNA binding and cleaving protein with greater sequence discrimination, we have designed dimeric proteins derived from engrailed homeodomain and calmodulin. Previous research by our group has shown that a hydrolytically active lanthanide binding site can be incorporated into a DNA binding motif. To understand protein-DNA interaction and improve the sequence selectivity of the chimeric complex, two lanthanide-binding homodimers were designed and expressed. One of the dimers, F2, is coupled together by a flexible polypeptide linker and the other, R7C, is a disulfide cross-linked cysteine mutant at the N-terminus. Studies of fluorescence of tryptophan residues document that the overall affinity for lanthanide and calcium is similar to traditional EF-hand peptides (1-10 µM). Metal titrations monitored by circular dichroism (CD) revealed that the secondary structures of the dimers contained a lower degree of α-helicity than the designed monomeric protein due to additional modifications, but because of their flexibility and their two active-site domain, hydrolytic activity was several folds faster than our previously designed proteins and peptides. Unlike earlier reports on our chimeras, F2 also demonstrated the capability to hydrolyze DNA in the presence of some biological relevant metal ions suggesting different cleavage mechanisms were carried out. Extensive DNA sequencing studies on cleavage patterns with oligonucleotide duplexes confirmed the unique sequence selectivity and kinetic properties of F2. Two engrailed homeodomain target sites, TAATTA, were favored for hydrolytic activity corresponding to one domain acting as a DNA anchor on the first target site while the other was an "opportunist" at recognizing the second site. Nonetheless, the hydrolytic behavior at the phosphodiester bond on a specific dsDNA sequence is in good agreement with the behavior of restriction endonucleases. Unlike restriction enzymes, metallated F2 has not only demonstrated the ability to cleave DNA plasmid, but it also excises the entire nucleotide on a selected sequence. This homodimer v Caroline and Alida showed me how to be "kick-ass" as a woman both literally and theoretically. They have become my second family whom I will always cherish. At home, I would like to thank you for the encouragement and perseverance of my aunt, Auntie Goat, who is no doubt the inspiration for my success nowadays. Nonetheless, no one else can compare to my devoting and loving parents, Kin Sang Lau and Cheuk Yut Wong. I would not be the person I am now without them as they have given up so much in order to provide me the best quality of life. They have showed me to be strong through tough times and celebrate the happiness in life. Last but not the least, I feel absolutely lucky that I have met my husband, Stephen Deyrup, who is my best friend and my companion in life. We have gone through many huddles together throughout all these years and his unconditional love, support, and trust for me remain untainted. His smile is my sunsh...

show abstract

Untitled

Cited by 16 publications

References 53 publications

Developments in wool carbonising

Developments in wool carbonising

Nanoscopic Structure of Complexes Formed between DNA and the Cell-Penetrating Peptide Penetratin

DNA sequence selectivity and kinetic properties of de novo designed metalloprotein dimers

Contact Info

Product

Resources

About