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Salvi, Neeta D.; George, L.; Eapen, Susan

doi:10.1023/a:1013889119887

Cited by 129 publications

(19 citation statements)

References 18 publications

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“…The slow differentiation of the meristemoids in Erlenmeyer flasks was very ideal to keep them as stock cultures. Rapid shoot initiation in solid medium and shoot multiplication in liquid medium in the present study was in concomitant with the reported on C. longa [33].…”

Section: Resultssupporting

confidence: 92%

Improved Clonal Propagation of Alpinia calcarata Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

Sudha¹,

George²,

Rameshkumar³

et al. 2012

AJPS

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An efficient and improved clonal propagation of Alpinia calcarata, a commercially important medicinal plant was established on Murashige and Skoog medium. The axillary shoot proliferation was achieved with maximum 5.2 ± 0.7 shoots in 92.8% of rhizome explants in medium with 2.0 mg/L 6-benzylamiopurine (BAP) and 0.2 mg/L indole-3-acetic acid (IAA). Axillary shoot buds (60%) upon subculture for 8 weeks in the same medium produced multiple shoot initials (12.1 ± 0.4) mediated with meristemoids (4.0 ± 0.5) and callus. A gradual reduction in the concentration of BAP or elimination of IAA was required for rapid induction of normal plants devoid of callus from propagules during subsequent subculture. Single clump of 3-4 multiple shoot initials during second subculture on medium with 1.0 mg/L BAP and 0.1 mg/L IAA yielded an average of 21 shoots which was best among different propagules tried. The shoot multiplication rate was further enhanced to 32 shoots when the similar propagules passed to third subculture on medium with 1.0 mg/L BAP alone. Clumps of multiple shoot initials upon subculture on medium with 1.0 mg/L BAP alone exhibited 10 fold multiplication rates. Use of liquid medium in culture bottles with polypropylene caps supported fast growth of the shoots and spontaneous root formation on 50% of the shoots. Shoots transferred to half-strength MS liquid medium with 0.2 mg/L of IAA and IBA was optimum for maximum roots (8.14 ± 1.34) in 100% shoots. The rooted plants were hardened in mist chamber showed 95% survival and well established in the field. The acclimatized plants showed rhizome formation after 4-6 weeks of growth under shade house. Volatile chemicals profile of the leaves, rhizome and root of the in vitro and conventionally propagated plants analyzed by gas chromatography-mass spectrometry were qualitatively and quantitatively similar. The analysis of growth characteristics of 36 month old in vitro and conventionally propagated plants showed a 50% increment of rhizome fresh biomass with prolific root and leaf growth in the former than the latter ones. The protocol described herein will have practical applications for the large scale production of uniform efficient plants for commercial cultivation of A. calcarata

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Section: Resultssupporting

confidence: 92%

Improved Clonal Propagation of Alpinia calcarata Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

Sudha¹,

George²,

Rameshkumar³

et al. 2012

AJPS

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“…Furthermore, larger microrhizomes are capable of survival in the field without any specific acclimatization procedure after this induction [9–11]. Microrhizomes were also found to produce secondary metabolites with antioxidant activity equaling or surpassing commercial dried powdered rhizome preparations of field-grown plants [12].…”

Section: Introductionmentioning

confidence: 99%

“…While red light has also been found to have a stimulatory effect on the phenylpropanoid pathway in Ocimum basilicum [25], it has been reported that light in the green spectrum enhances the production of phenolic compounds, flavonoids, and antioxidant activity in callus cultures of A. absinthium [20]. In addition, in the context of microrhizome formation, sucrose concentration has been reported to be a major factor in several Curcuma species [9–11]. …”

Section: Introductionmentioning

confidence: 99%

Factors Affecting the Accumulation of Curcumin in Microrhizomes ofCurcuma aromaticaSalisb

Zhang

Sun

et al. 2015

BioMed Research International

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Curcuminoids, and mainly curcumin, are potential therapeutic agents for the prevention of various diseases; however, little is known about the factors that influence their accumulation in Curcuma species. In this study, the effects of factors such as sucrose concentration, different ratios of 6-benzylaminopurine (6-BA) and α-naphthalene acetic acid (NAA), and light quality on the accumulation of curcumin and other curcuminoids in Curcuma aromatica were investigated. Microrhizomes grown on media containing 3% sucrose produced more curcumin and other curcuminoids than those grown on higher concentrations. Moreover, when compared to other ratios of 6-BA and NAA, microrhizomes induced on 3% sucrose media supplemented with 3.0 mg/L 6-BA and 0.5 mg/L NAA produced more curcumin and other curcuminoids; however, the amount was less than in microrhizomes grown on 3% sucrose alone. We determined that a 5% sucrose medium supplemented with 3.0 mg/L of 6-BA and 0.5 mg/L of NAA enhanced the levels of curcumin and curcuminoids and that exposure to red light further increased production.

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“…NARI-H-15 via micropropagation than organogenesis. In a similar fashion, low concentration of mT was resulted more roots in Musca [33] and turmeric [21] …”

Section: Rooting and Acclimatization Responsementioning

confidence: 90%

“…The mT group compound can be considered as an alternative to other commonly used cytokinins in regeneration of recalcitrant plant species. In earlier studies, mT group cytokinin and its derivatives have a comparable effect on in vitro regeneration of sugar beet [20], Curcuma longa [21], Aloe polyphylla [22], banana cv. Williams [23].…”

Section: Introductionmentioning

confidence: 99%

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (Carthamus tinctorius L.) cv. NARI-H-15

Vijayakumar¹,

Ponmanickam²,

Samuel³

et al. 2017

AJPS

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The effect of meta-Topolin (mT) was assessed to develop a reliable protocol for efficient plant regeneration of safflower (Carthamus tinctorius L.) cv.NARI-H-15. For micropropagation, 7 -9 days old shoot-tip explants cultured on MS basal medium supplemented with 3.0 mg/L meta-Topolin (mT) + 0.5 mg/L CPPU showed 97.7% adventitious shoot formation (42.4 shootlets) than node after 45 days of culture. For organogenesis, the seedling explants of immature leaf cultured on 1.5 mg/L CPPU or 1.5 mg/L NAA fortified medium produced high amount of callus than cotyledon and stem calli after 60 days of culture. However, MS basal medium fortified with 4.0 mg/L mT + 1.5 mg/L CPPU was found beneficial to stimulate 100% organogenic response (74.7 shootlets) from immature leaf calli than cotyledon and stem derived calli after 45 days of culture. The healthy plantlets obtained from micropropagation and organogenesis process cultured on 1/4 MS basal salts, 1.5% sucrose (w/v) and 0.8% agar (w/v) medium supplemented with NAA (1.5 mg/L) and mT (0.1 mg/L) produced maximum of 96% (12.8 rootlets) and 84% (7.3 rootlets) adventitious rooting, respectively than mT and CPPU tested medium. However, maximum of 67% and 42% survival rate was noticed when in vitro raised plants from micropropagation and organogenesis were hardened in pots containing soil mix and maintained under green house condition. This optimized regeneration protocol might be helpful in regeneration of new genotypes and cultivars of safflower to improve agronomic traits through in vitro selection process and Agrobacterium-mediated genetic transformation system. Keywords

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Cited by 129 publications

References 18 publications

Improved Clonal Propagation of <i>Alpinia calcarata</i> Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

Improved Clonal Propagation of <i>Alpinia calcarata</i> Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

Factors Affecting the Accumulation of Curcumin in Microrhizomes ofCurcuma aromaticaSalisb

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (<i>Carthamus tinctorius</i> L.) cv. NARI-H-15

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Untitled

Cited by 129 publications

References 18 publications

Improved Clonal Propagation of &lt;i&gt;Alpinia calcarata&lt;/i&gt; Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

Improved Clonal Propagation of &lt;i&gt;Alpinia calcarata&lt;/i&gt; Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

Factors Affecting the Accumulation of Curcumin in Microrhizomes ofCurcuma aromaticaSalisb

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (&lt;i&gt;Carthamus tinctorius&lt;/i&gt; L.) cv. NARI-H-15

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Improved Clonal Propagation of <i>Alpinia calcarata</i> Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

Improved Clonal Propagation of <i>Alpinia calcarata</i> Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (<i>Carthamus tinctorius</i> L.) cv. NARI-H-15