Previous bone collagen carbon and nitrogen isotopic studies of Late Pleistocene European cave bears (Ursus spelaeus) have shown that these bears frequently had low nitrogen isotope values, similar to those of herbivores and indicating either unusual physiology related to hibernation or a herbivorous diet. Isotopic analysis of animal bone from the Peştera cu Oase (Cave with Bones), Romania, shows that most of its cave bears had higher nitrogen isotope values than the associated herbivores and were, therefore, omnivorous. The Oase bears are securely identified as cave bears by both their morphology and DNA sequences. Although many cave bear populations may have behaved like herbivores, the Oase isotopic data demonstrate that cave bears were capable of altering their diets to become omnivores or even carnivores. These data therefore broaden the dietary profile of U. spelaeus and raise questions about the nature of the carnivore guild in Pleistocene Europe.carbon isotopes ͉ nitrogen isotopes ͉ diets ͉ ancient DNA L ate Pleistocene cave bear (Ursus spelaeus) diet and physiology is not well known, although based on skull, mandible, and tooth morphology, they have been inferred to have been largely herbivorous (1-4). Moreover, isotopic studies of central and western European cave bears have shown that, in most cases where their values are compared with herbivores and carnivores from the same site, they have ␦ 15 N values that plot with, or lower than, herbivores (Table 1). These dietary inferences are in the context of bears that had a period of dormancy, similar to modern black bears (Ursus americanus) and brown bears (Ursus arctos), given the abundance of hibernation nests in European caves. This pattern has been interpreted in several ways.Based Table 1), is that some cave bears were largely herbivorous, as are some modern bears, but that other cave bears were more carnivorous and hence exhibit ␦ 15 N values similar to those of many modern omnivorous U. arctos and U. americanus. In this interpretation, the variation in trophic level indicated by ␦ 15 N values would reflect ecological plasticity among U. spelaeus, in which they were more or less carnivorous depending on available food resources, body-size requirements, interspecific competition, seasonality, and temperature.Regardless of the cause, 86.1% of the available individual European adult cave bear ␦ 15 N values (n ϭ 101) are Ͻ6.0‰, and hence within the basically vegetarian range [the number rises to 95.6%, n ϭ 91 without Hilderbrand et al.'s (14) 10 specimens] (Table 1). In the context of this distribution of ␦ 15 N values, routine isotopic assessment of a cave bear metapodial associated with an early modern human cranium in the Late Pleistocene Peştera cu Oase, Caraş-Severin, southwestern [supporting information (SI) Text and SI Figs. 4 and 5], provided an anomalously high ␦ 15 N value of 7.8‰ (SI Table 2, Oase O34-232). To further evaluate this result and address the cause of the generally low cave bear ␦ 15 N values, we measured the isotope values of c...