[14C]Formate labelling of bases of nucleic acids in respiring slices of rat tissues

Mannell, W. A.; Rossiter, R. J.

doi:10.1042/bj0610418

Cited by 18 publications

(1 citation statement)

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“…In brain slices [14C]formate is incorporated into nucleic acids (Mannell & Rossiter, 1955), [14C]glycine into phosphoribosylformylglycine amide (Howard et al, 1970) and [14C]adenine and ["4C]adenosine into adenine nucleotides (Fom & Krishna, 1971; Sharma & Singh, 1970; Shimizu et al, 1969). Santos et al (1968) measured the activities of a number of enzymes of purine metabolism in extracts of rat cerebral cortex, and also determined the ability of cortical slices to take up a variety of purine ribonucleotide precursors and convert these into nucleotides and nucleic acids.…”

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Purine ribonucleotide biosynthesis, interconversion and catabolism in mouse brain in vitro

Wong

Henderson

1972

Biochemical Journal

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The relative rates of the synthetic, interconversion and catabolic reactions of purine metabolism in chopped mouse cerebrum were studied. The rates of incorporation of [(14)C]adenine and [(14)C]hypoxanthine into purine ribonucleotides were much less than the potential activities of adenine phosphoribosyltransferase and hypoxanthine phosphoribosyltransferase, and the rates of incorporation were stimulated by the addition of guanosine to the incubation mixture. The availability of ribose phosphates may be a limiting factor for the formation of ribonucleotides from purine bases. The rate of incorporation of [(14)C]adenosine into purine ribonucleotides was at least seven- to eight-fold higher than that of adenine. The radioactivity in adenine ribonucleotides synthesized from adenine and hypoxanthine was about 100- and ten-fold respectively higher than that in the radioactive guanine ribonucleotides. The conversion of inosinate into guanine ribonucleotides was probably limited by the amount of inosinate available, and the conversion of adenine ribonucleotides into guanine ribonucleotides was probably limited by the activity of adenylate deaminase. The rate of catabolism of [(14)C]adenosine was low in comparison with its rate of utilization for ribonucleotide synthesis. A fraction of the [(14)C]hypoxanthine was catabolized to xanthine and urate. [(14)C]Guanine was completely converted into xanthine, mostly by the guanine deaminase that was released during incubation of chopped mouse cerebrum.

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confidence: 99%