An injection-seeded Ho:YAG laser at 2090 nm with changeable pulse repetition frequency (PRF) is demonstrated. Containing a Ho:YAG nonplanar ring oscillator (NPRO) seed, a slave laser, and a single-pass amplifier, the laser delivered single-frequency pulses with energy ranging from 31.4 to 12.7 mJ. The corresponding pulse duration and PRF varied between 102–215 ns and 150–750 Hz, respectively. To the best of our knowledge, this is the highest PRF ever obtained from a single-frequency Ho:YAG laser.