2004
DOI: 10.1071/rdv16n1ab151
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151a Simple and Fast Method for Concurrent Differential Staining and Tunel Labelling of Bovine Blastocysts

Abstract: Currently techniques of TUNEL labelling for detection of apoptosis and differential staining for counting the ratio of inner cell mass (ICM) to trophoectoderm (TE) cells are used separately for assessment of embryo quality in different species. The majority of these techniques are antibody-based, and time-consuming, frequently giving inconsistent results. Here we report on the development of a simple and fast method for simultaneous differential staining and TUNEL labelling of bovine embryos. Cleaved embryos p… Show more

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