Many of the studies on in vitro fertilization in South American camelids has culminated in the evaluation of the development to blastocyst. Initially work has been done with gametes collected from post-mortem animal, but recent work is already reporting the recovery of gametes from live females and males. Pregnancies have been achieved with in vitro embryos in alpacas and llamas, with gametes recovered from post-morten animals and with gametes collected from living animals on llamas, the semen was collected by electroejaculation and the oocytes were aspirated from the ovaries exposed by laparotomy. Likewise, the birth of a male llama of gametes recovered from post-mortem animals has been achieved. However, further studies are needed to improve the efficiency of this biotechnology, standardize follicular aspiration protocols guided by ultrasonography, synchronism between the embryo and the recipient, train technicians trained in embryo transfer in camelids, overcoming these issues no doubt that the results of pregnancy and birth will improve by applying this biotechnology in South American camelids.