15N-Glycine as a Tracer to Study Protein Metabolism in Vivo

Assimon, Sue Anne; Stein, T. P.

doi:10.1016/b978-0-12-519570-6.50015-2

Cited by 14 publications

(8 citation statements)

References 63 publications

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“…An early study compared the protein requirements in young and old animals. 59 Results showed a positive correlation between whole body protein turnover and dietary protein level in both youngadult and geriatric Beagles. Protein:DNA ratios were maximized when young adult dogs were fed a semipurified diet containing 12.4% protein.…”

Section: Protein and Amino Acidsmentioning

confidence: 94%

Geriatrics

Case

Daristotle

Hayek

et al. 2011

Canine and Feline Nutrition

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Section: Protein and Amino Acidsmentioning

confidence: 94%

Geriatrics

Case

Daristotle

Hayek

et al. 2011

Canine and Feline Nutrition

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“…To calculate protein synthesis and degradation, whole-body protein turnover was measured using the 15 N-glycine single-dose urea end-product method (Assimon and Stein, 1992;Wessels et al, 1997). A single dose of 0.4 g 15 N-glycine (98 atom percent excess; Sigma-Aldrich Chemical Company, St. Louis, MO) dissolved in 100 mL of water was infused abomasally as a pulse dose to each steer 48 h prior to the end of each period.…”

Section: Methodsmentioning

confidence: 99%

Methionine as a methyl group donor in growing cattle

Löest

Titgemeyer

Metre

et al. 2002

Journal of Animal Science

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Holstein steers were used in two 5 x 5 Latin square experiments to evaluate the sparing of methionine by alternative sources of methyl groups (betaine and choline). Steers were housed in metabolism crates and limit-fed a soybean hull-based diet high in rumen degradable protein. To increase energy supply, ruminal infusions of volatile fatty acids and abomasal infusions of glucose were provided. An amino acid mixture, limiting in methionine, was infused abomasally to ensure that nonsulfur amino acids did not limit protein synthesis. Treatments for Exp. 1 were abomasal infusion of 1) water, 2) 2 g/d L-methionine, 3) 1.7 g/d L-cysteine, 4) 1.6 g/d betaine, and 5) 1.7 g/d L-cysteine + 1.6 g/d betaine. Treatments for Exp. 2 were abomasal infusion of 1) water, 2) 2 g/d L-methionine, 3) 8 g/d betaine, 4) 16 g/d betaine, and 5) 8 g/d choline. In both experiments, nitrogen retention increased in response to methionine (P < 0.05), demonstrating a deficiency of sulfur amino acids. Responses to cysteine, betaine, and choline were all small and not significant. The lack of response to cysteine indicates that the response to methionine was not due to transsulfuration to cysteine or that cysteine supply did not alter the flux of methionine through transsulfuration. The lack of response to betaine suggests that the steers' needs for methyl groups were met by the dietary conditions or that betaine was relatively inefficient in increasing the remethylation of homocysteine to methionine and, thereby, reducing the synthesis of cysteine from homocysteine. Under our experimental conditions, responses to methionine were likely due to a correction of a deficiency of methionine per se rather than of methyl group donors.

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“…The mechanism by which gum arabic ameliorates CRF is uncertain. It was reported that gum arabic increased faecal nitrogen excretion and decreased both urea production and urea nitrogen recycling [4]. A study in animal models of experimental CRF showed that consumption of diets containing fermentable carbohydrates resulted in a greater faecal nitrogen excretion, coupled with a reduction in serum urea concentration [5].…”

Section: Introductionmentioning

confidence: 99%

The effects of gum arabic oral treatment on the metabolic profile of chronic renal failure patients under regular haemodialysis in Central Sudan

Ali

Fadlalla

et al. 2008

Natural Product Research

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This study aimed at assessing the effect of gum arabic (Acacia senegal) oral treatment on the metabolic profile of chronic renal failure (CRF) patients. A total of 36 CRF patients (under regular haemodialysis) and 10 normal subjects participated in this study. The patients were randomly allocated into three groups-group A: 12 CRF patients under low-protein diet (LPD) (<40 g day(-1)) and gum arabic (50 g day(-1)) treatment; group B: 14 CRF patients under LPD and gum arabic, iron (ferrous sulphate, 200 mg day(-1)) and folic acid (5 mg day(-1)) treatment; group C (control group): 10 CRF patients under LPD and iron and folic acid treatment and group D: 10 normal volunteers (on normal diet) under daily dose of 50 g gum arabic. Each of the above treatments was continued for three consecutive months. Blood samples were collected from each subject before treatment and twice per month "pre-dialysis" for 3 months. Biochemical parameters measured were: serum urea, serum creatinine, serum uric acid, serum calcium and serum phosphorus. By the end of the 3 months of treatment, serum urea levels significantly decreased by 31.2 and 44.18% for group A and B, respectively, compared with the baseline (0.01 < p < 0.001) and control group (p < 0.05). Serum creatinine levels significantly decreased in the groups of gum users (A, B and D) by 9.94, 12.65 and 11.7%, respectively, compared with the control group (p < 0.001). There was a significant decrease (p < 0.05) in serum uric acid levels by 14 and 19.9% for group A and B, respectively, compared with the baseline. Serum calcium levels increased by 12.64, 15.75 and 8.75% for group A, B and D, respectively, and these increases were significantly different (0.05 < p < 0.001) from baseline and control group for groups A and B. Serum phosphorus levels significantly decreased by 22.54% for group A, 17.69% for group B and 7.71% for group D, compared with the baseline (0.05 < p < 0.001). From this study, we conclude that oral administration of gum arabic could conceivably alleviate adverse effects of CRF.

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15N-Glycine as a Tracer to Study Protein Metabolism in Vivo

Cited by 14 publications

References 63 publications

Geriatrics

Geriatrics

Methionine as a methyl group donor in growing cattle

The effects of gum arabic oral treatment on the metabolic profile of chronic renal failure patients under regular haemodialysis in Central Sudan

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