16-Kilodalton Rice Protein Is One of the Major Allergens in Rice Grain Extract and Responsible for Cross-Allergenicity between Cereal Grains in the Poaceae Family

Urisu, Atsuo; Yamada, Kazue; Masuda, Shōgo; Komada, Hidekatsu; Wada, Eiko; Kondo, Yasuto; Horiba, Fumiya; Tsuruta, Mitsutoshi; Yasaki, Takehiko; Yamada, Masanori; Torii, Shinpei; Nakamura, Ryô

doi:10.1159/000235502

Cited by 82 publications

(49 citation statements)

References 16 publications

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“…However, it is still uncertain whether such 'hypo-allergenic rice' seeds obtained from the transgenic rice plants are tolerable to patients allergic to rice, because even a small amount of residual allergens could elicit an allergic reaction in patients. Furthermore, some patients have been reported to be allergic to some other components of rice seed proteins [6]. Further studies on the improvement of the antisense allergen genes and identification of the other allergenic proteins and their genes are in progress.…”

Section: Y Tada Et Al/febs Letters 391 (1996) 341-345mentioning

confidence: 99%

“…Several studies on allergic reaction to rice have indicated that patients' sera contain immunoglobulin E (IgE) reacting to rice proteins, and that soluble proteins, albumins and globulins have a high degree of allergenic activity [3,4]. The authors have isolated a rice seed protein of about 16 kDa with reactivity for IgE of several rice-allergic patients [5], and the 16 kDa protein was later identified as one of the major allergens in rice seed [6]. Monoclonal and polyclonal antibodies raised against the 16 kDa allergen were shown to cross-react with the 14--16 kDa allergens [7].…”

Section: Introductionmentioning

confidence: 99%

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Reduction of 14–16 kDa allergenic proteins in transgenic rice plants by antisense gene

et al. 1996

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An antisense gene strategy was applied to suppress the 14-16 kDa allergen gene expression in maturing rice seeds. Gene constructs producing antisense RNAs of the 16 kDa allergen under the control of some rice seed-specific promoters were introduced into rice by electroporation. Immunoblot and RNA blot analyses of the seeds from the transgenic rice plants using the allergen-specific monoclonal antibody and a sequencespecific antisense RNA probe demonstrated that the 14-16 kDa allergen proteins and their transcripts of the seeds from several transgenic lines were present in much lower in amounts than those of the seeds from parental wild-type rice. The high levels of reduction observed were stably inherited in at least three generations.Key words: Rice allergen; Transgenic rice; Antisense RNA has been used experimentally to inhibit gene expression in bacteria, yeast, plant and animal cells [12], and the antisense strategy has also been reported to be practically applicable to transgenic crop plants, to which genes that produce antisense RNA are introduced to suppress the gene expression [13,14]. In the present study, the antisense genes for the 16 kDa allergen were introduced into rice by electroporation, and specific suppression of the 14-16 kDa allergen gene expression, resulting in the reduction of corresponding allergenic proteins in the mature seeds, was examined for regenerated transgenic rice plants and their progeny. The results show that antisense RNA markedly reduced the mRNA and protein contents of the 14-16 kDa allergens in the transgenic rice seeds. Materials and methods

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Section: Y Tada Et Al/febs Letters 391 (1996) 341-345mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Reduction of 14–16 kDa allergenic proteins in transgenic rice plants by antisense gene

et al. 1996

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“…Several rice proteins with molecular masses of 9, 14-16, 26, 33 and 60 kDa have so far been reported to be recognized by the serum IgE of patients showing hypersensitive reactions to rice ingestion. These rice allergens have been identified as non-specific lipid transfer protein-1 (nsLTP1), 29) -amylase/trypsin inhibitors, 25) -globulin, 30) glyoxalase I (GlyI) 24) and granule-bound starch synthetase (GBSSI). 26) Most of these allergens have been used to assess the safety of GM rice.…”

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confidence: 99%

Evaluation of Extraction Solutions for Biochemical Analyses of the Proteins in Rice Grains

Lang

Kagiya

Ohnishi‐Kameyama

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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The influence of different extraction solutions on the proteins extracted from rice grains was investigated. The largest amounts of salt-soluble proteins were extracted with solutions supplemented with Tris-HCl at pH 8.0. Rice allergens were analyzed by multiplex immunodetection. Except for -globulin extracted with the solutions at pH 8.0, which showed a low-molecularweight band besides the main band, no significant solution-dependent difference among the allergens was found. Total proteins were extracted with four kinds of solution. The extraction of the basic subunit of glutelin was found to be SDS-dependent, and more protein was obtained with extraction solutions supplemented with SDS. The contents of -globulin and -amylase/trypsin inhibitors were higher in the extracts without SDS than with SDS. We conclude from the present data that, in order to obtain comparable data from rice grain saltsoluble and total protein analyses, differences in the protein extraction efficiency of solutions used should be taken into consideration.

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“…Among rice proteins, 16 kD protein has been known as one of the major allergens in rice grain, and it also inhibits serum IgE binding to other cereal grain extracts including wheat, corn and millet (Urisu et al, 1991). These rice allergenic proteins are rice seed albumin and exhibit microheterogeneity in molecular weight (14-16 kD) (Matsuda et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Isolation of rice allergenic cDNA clones from a rice cDNA library by immunoscreening with a polyclonal antibody specific to 16 kD rice allergenic protein

Kim

Kim²,

Lee³

et al. 1999

Exp Mol Med

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Clinical cases of type-1 hypersensitive reaction to rice (Oryza sativa) have been reported in western countries as well as in Japan. Among rice proteins, 14-16 kD globulin proteins encoded by multiple gene family have been identified as major rice allergens. In this study, a rice cDNA library was constructed using λ λ λ λ UniZap vector and screened with a rat anti-16 kD globulin protein polyclonal antibody in order to isolate Korean rice allergenic cDNA clones. Five independent cDNA clones, termed RAK1-5, were obtained after second rounds of plaque assay and immunoblot analysis. These clones encoded 13-19 kD recombinant proteins upon IPTG induction, which were identified by the polyclonal antibody in immunoblot analysis. DNA sequencing analysis showed that RAK1-4 have 99% sequence homology with RA5b, and RAK5 is closely related with RA14c. This result indicated that RA5b gene is widely distributed in our cDNA library among other possible rice allergenic genes, and more study is needed to isolate heterogeneous or novel rice allergen genes.

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16-Kilodalton Rice Protein Is One of the Major Allergens in Rice Grain Extract and Responsible for Cross-Allergenicity between Cereal Grains in the Poaceae Family

Cited by 82 publications

References 16 publications

Reduction of 14–16 kDa allergenic proteins in transgenic rice plants by antisense gene

Reduction of 14–16 kDa allergenic proteins in transgenic rice plants by antisense gene

Evaluation of Extraction Solutions for Biochemical Analyses of the Proteins in Rice Grains

Isolation of rice allergenic cDNA clones from a rice cDNA library by immunoscreening with a polyclonal antibody specific to 16 kD rice allergenic protein

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