1974
DOI: 10.1016/0076-6879(74)31019-1
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[16] Preparation of impermeable ghosts and inside-out vesicles from human erythrocyte membranes

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Cited by 1,005 publications
(448 citation statements)
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“…The animals were bled and blood was collected in citrated test tubes. The plasma was removed and the red blood cells were washed as in [9]. The dehydroergosterol was incorporated into plasma lipoproteins as in [ 10].…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The animals were bled and blood was collected in citrated test tubes. The plasma was removed and the red blood cells were washed as in [9]. The dehydroergosterol was incorporated into plasma lipoproteins as in [ 10].…”
Section: Methodsmentioning
confidence: 99%
“…The plasma containing dehydroergosterol was incubated at 37°C for 20 h with the red blood cells as in [10]. The red blood cells were then treated with trinitrobenzenesulfonic acid as above (except that 23°C and 37°C were used), lysed, washed 3 times, and resealed as right-side out vesicles in phosphate-buffered saline [9]. Protein was determined by the method in [ 11].…”
Section: Methodsmentioning
confidence: 99%
“…21 Right-side-out erythrocyte ghosts were prepared from hypotonically lysed red blood cells. 36 Erythrocyte ghost phospholipid contents were determined by phosphate analysis 34 after extraction 37 and digestion of the lipids. 35 Ghosts and virosomes were added to a quartz microcuvette containing HBS.…”
Section: Preparation Of Virosomesmentioning
confidence: 99%
“…The precipitate was finally suspended in a small amount of 0.25 M sucrose containing 10 mM Tris-HCl (pH 7.4), frozen in liquid N2, and stored at -70°C until use. Orientation of membrane vesicles was determined according to [11], about 50°70 of the population being estimated to be insideout. Details of the procedure and characterization of the sarcolemma vesicles will be reported elsewhere (in preparation).…”
Section: Preparation Of Sarcolemmal Vesicles From Rat Heartmentioning
confidence: 99%