16 years research on lactic acid production with yeast – ready for the market?

Sauer, Michael; Porro, Danilo; Mattanovich, Diethard; Branduardi, Paola

doi:10.1080/02648725.2010.10648152

Cited by 111 publications

(97 citation statements)

References 79 publications

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“…() demonstrated that the yield of lactic acid changed significantly depending on the strain and LDH gene used. The lactic acid titres achieved in the present study were much lower than those reported in other modified yeast strains (reviewed in Sauer et al ., ), but higher than that for S. cerevisiae W303‐1A transformed with a heterologous LDH (Branduardi et al ., ). It is reported that S. cerevisiae W303‐1A expressing a Bacillus taurus LDH has a lactic acid titre of 20 mg L −1 after 30 h of growth in YNB glucose 2% medium (Branduardi et al ., ).…”

Section: Resultsmentioning

confidence: 83%

Lactic acid production in Saccharomyces cerevisiae is modulated by expression of the monocarboxylate transporters Jen1 and Ady2

et al. 2012

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We aimed to manipulate the metabolism of Saccharomyces cerevisiae to produce lactic acid and search for the potential influence of acid transport across the plasma membrane in this process. Saccharomyces cerevisiae W303-1A is able to use l-lactic acid but its production in our laboratory has not previously been detected. When the l-LDH gene from Lactobacillus casei was expressed in S. cerevisiae W303-1A and in the isogenic mutants jen1∆, ady2∆ and jen1∆ ady2∆, all strains were able to produce lactic acid, but higher titres were achieved in the mutant strains. In strains constitutively expressing both LDH and JEN1 or ADY2, a higher external lactic acid concentration was found when glucose was present in the medium, but when glucose was exhausted, its consumption was more pronounced. These results demonstrate that expression of monocarboxylate permeases influences lactic acid production. Ady2 has been previously characterized as an acetate permease but our results demonstrated its additional role in lactate uptake. Overall, we demonstrate that monocarboxylate transporters Jen1 and Ady2 are modulators of lactic acid production and may well be used to manipulate lactic acid export in yeast cells.

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Section: Resultsmentioning

confidence: 83%

Lactic acid production in Saccharomyces cerevisiae is modulated by expression of the monocarboxylate transporters Jen1 and Ady2

et al. 2012

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“…Although this demonstrated the feasibility of producing d -xylonate with yeast, the titres and rates were low compared to those obtained with bacteria (see above) or for other acids produced in S. cerevisiae , e.g. lactate >100 g l −1 (Sauer et al 2010). …”

Section: Recombinant Yeast For D-xylonate Productionmentioning

confidence: 88%

Microbial d-xylonate production

Toivari

Nygård

Penttilä

et al. 2012

Appl Microbiol Biotechnol

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d-Xylonic acid is a versatile platform chemical with reported applications as complexing agent or chelator, in dispersal of concrete, and as a precursor for compounds such as co-polyamides, polyesters, hydrogels and 1,2,4-butanetriol. With increasing glucose prices, d-xylonic acid may provide a cheap, non-food derived alternative for gluconic acid, which is widely used (about 80 kton/year) in pharmaceuticals, food products, solvents, adhesives, dyes, paints and polishes. Large-scale production has not been developed, reflecting the current limited market for d-xylonate. d-Xylonic acid occurs naturally, being formed in the first step of oxidative metabolism of d-xylose by some archaea and bacteria via the action of d-xylose or d-glucose dehydrogenases. High extracellular concentrations of d-xylonate have been reported for various bacteria, in particular Gluconobacter oxydans and Pseudomonas putida. High yields of d-xylonate from d-xylose make G. oxydans an attractive choice for biotechnical production. G. oxydans is able to produce d-xylonate directly from plant biomass hydrolysates, but rates and yields are reduced because of sensitivity to hydrolysate inhibitors. Recently, d-xylonate has been produced by the genetically modified bacterium Escherichia coli and yeast Saccharomyces cerevisiae and Kluyveromyces lactis. Expression of NAD+-dependent d-xylose dehydrogenase of Caulobacter crescentus in either E. coli or in a robust, hydrolysate-tolerant, industrial Saccharomyces cerevisiae strain has resulted in d-xylonate titres, which are comparable to those seen with G. oxydans, at a volumetric rate approximately 30 % of that observed with G. oxydans. With further development, genetically modified microbes may soon provide an alternative for production of d-xylonate at industrial scale.

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“…The first paper on metabolic engineering of a yeast strain to produce LA was published in 1994 (Sauer et al, 2010). Candida sonorensis is a methylotrophic yeast that can convert glucose and pentose sugars including xylose and arabinose to ethanol.…”

Section: Lactic Acid Production By Yeastsmentioning

confidence: 99%

Microbial production of lactic acid: the latest development

Juturu

2015

Critical Reviews in Biotechnology

191

113

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Lactic acid is an important platform chemical for producing polylactic acid (PLA) and other value-added products. It is naturally produced by a wide spectrum of microbes including bacteria, yeast and filamentous fungi. In general, bacteria ferment C5 and C6 sugars to lactic acid by either homo- or hetero-fermentative mode. Xylose isomerase, phosphoketolase, transaldolase, l- and d-lactate dehydrogenases are the key enzymes that affect the ways of lactic acid production. Metabolic engineering of microbial strains are usually needed to produce lactic acid from unconventional carbon sources. Production of d-LA has attracted much attention due to the demand for producing thermostable PLA, but large scale production of d-LA has not yet been commercialized. Thermophilic Bacillus coagulans strains are able to produce l-lactic acid from lignocellulose sugars homo-fermentatively under non-sterilized conditions, but the lack of genetic tools for metabolically engineering them severely affects their development for industrial applications. Pre-treatment of agriculture biomass to obtain fermentable sugars is a pre-requisite for utilization of the huge amounts of agricultural biomass to produce lactic acid. The major challenge is to obtain quality sugars of high concentrations in a cost effective-way. To avoid or minimize the use of neutralizing agents during fermentation, genetically engineering the strains to make them resist acidic environment and produce lactic acid at low pH would be very helpful for reducing the production cost of lactic acid.

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16 years research on lactic acid production with yeast – ready for the market?

Cited by 111 publications

References 79 publications

Lactic acid production in Saccharomyces cerevisiae is modulated by expression of the monocarboxylate transporters Jen1 and Ady2

Lactic acid production in Saccharomyces cerevisiae is modulated by expression of the monocarboxylate transporters Jen1 and Ady2

Microbial d-xylonate production

Microbial production of lactic acid: the latest development

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