16S Ribosomal RNA Analysis of Filibacter limicola Indicates a Close Relationship to the Genus Bacillus

Abstract

The phylogenetic relationship of the Gram-negative, filamentous gliding bacterium Filibacter limicola was analysed by 16S rRNA oligonucleotide cataloguing. In contrast to the proposed membership of this asporogenous species in the Flexibacteriaceae, Filibacter limicola clusters phylogenetically with the Gram-positive eubacteria Bacillus pasteurii, Sporosarcina ureae and the asporogenous species Planococcus citreus. The genetic relationship is supported by several common phenotypic properties.

Sporosarcina aquimarina sp. nov., a bacterium isolated from seawater in Korea, and transfer of Bacillus globisporus (Larkin and Stokes 1967), Bacillus psychrophilus (Nakamura 1984) and Bacillus pasteurii (Chester 1898) to the genus Sporosarcina as Sporosarcina globispora comb. nov., Sporosarcina psychrophila comb. nov. and Sporosarcina pasteurii comb. nov., and emended description of th.

“…The rRNA group 2 of the genus Bacillus comprises round-spore-forming bacilli, which form a radiation of the cluster distinct from members of other rRNA groups (Ash et al, 1991 ;Stackebrandt et al, 1987). However, phylogenetic analysis based on 16S rRNA sequences has shown that this group also comprises some non-Bacillus-type organisms, such as the genera Caryophanon, Planococcus, Filibacter and Sporosarcina (Clausen et al, 1985 ;Farrow et al, 1992Farrow et al, , 1994Stackebrandt et al, 1987). Members of the Bacillus rRNA group 2 are chemotaxonomically characterized by containing -lysine or -ornithine at position 3 of the peptide subunit of the peptidoglycan (Rheims et al, 1999 ;Stackebrandt et al, 1987).…”

Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with L-lysine in the cell wall, and reclassification of Bacillus marinus Rüger 1983 . as mMrinibacillus marinus gen nov., comb. nov.

“…Bacillus species belonging to this group form the radiation of a cluster distinct from members of other rRNA groups of the genus Bacillus (Ash et al, 1991 ;Stackebrandt et al, 1987). However, it should be noted that this group also consists of a phylogenetic jumble with some non-Bacillus-type organisms, such as the genera Caryophanon, Planococcus, Filibacter and Sporosarcina (Clausen et al, 1985 ;Farrow et al, 1992Farrow et al, , 1994Stackebrandt et al, 1987). On the basis of these properties, it has been recognized that the - J.-H. Yoon and others lysine-and -ornithine-containing bacilli belonging to rRNA group 2 may have to be taxonomically reevaluated (Farrow et al, 1994 ;Rheims et al, 1999).…”

Bacillus silvestris sp. nov., a new member of the genus Bacillus that contains lysine in its cell wall

“…Analysis of 16s rRNA of Gram-positive bacteria from other genera indicated that this Bacillus group also contains non-Bacillus-type organisms, such as members of Caryophanon and Planococcus (Stackebrandt et al, 1987;Farrow et al, 1994), Filibacter (Clausen et al, 1985) and Sporosarcina (Pechman et al, 1976;Farrow et al, 1992).…”