16s rRNA-based phylogenetic analyses of Elizabethkingia anophelis: Detection of Elizabethkingia anophelis, a rare infectious agent from blood and determination of antibiotic susceptibility in Turkey

Kadı, Hamza; Çaycı, Yeliz Tanrıverdi; Yener, Nazik; Vural, Demet Gür; Bılgın, Kemal; Bırınci, Asuman

doi:10.1016/j.ijmmb.2022.07.004

Cited by 4 publications

(8 citation statements)

References 12 publications

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“…This was evidenced by our own study, as well as others, where MALDI-ToF frequently misidentified E. anophelis as E. meningosepticum [41,46,48]. The use of 16S rRNA sequences has been shown to be limited in its taxonomic utility as well [48]. The fact that misidentification via conventional methodologies is so prevalent in the literature may indicate E. anophelis is an underrepresented pathogen responsible for more disease in humans than previously attributed [46].…”

Section: Discussionmentioning

confidence: 64%

“…Furthermore, standard databases are limited to only a few Elizabethkingia isolates, often falsely defaulting to E. meningoseptica or E. miricola [41]. This was evidenced by our own study, as well as others, where MALDI-ToF frequently misidentified E. anophelis as E. meningosepticum [41,46,48]. The use of 16S rRNA sequences has been shown to be limited in its taxonomic utility as well [48].…”

Section: Discussionmentioning

confidence: 86%

“…The fact that misidentification via conventional methodologies is so prevalent in the literature may indicate E. anophelis is an underrepresented pathogen responsible for more disease in humans than previously attributed [ 46 ]. These limitations highlight the need for updating standard MALDI-ToF databases, as well as for thorough, enhanced identification methodologies that utilize a combination of widely adopted bacterial identification methods like 16s rDNA sequencing in conjunction with biochemical testing [ 41 , 46 , 48 ]. Moreover, whole genomic sequence analysis and average nucleotide identity as a complementary method may be used to correctly identify E. anophelis [ 46 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

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Elizabethkingia anophelis MSU001 Isolated from Anopheles stephensi: Molecular Characterization and Comparative Genome Analysis

Chen,

Pham,

Terrapon

et al. 2024

Microorganisms

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Elizabethkingia anophelis MSU001, isolated from Anopheles stephensi in the laboratory, was characterized by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-ToF/MS), biochemical testing, and genome sequencing. Average nucleotide identity analysis revealed 99% identity with the type species E. anophelis R26. Phylogenetic placement showed that it formed a clade with other mosquito-associated strains and departed from a clade of clinical isolates. Comparative genome analyses further showed that it shared at least 98.6% of genes with mosquito-associated isolates (except E. anophelis As1), while it shared at most 88.8% of common genes with clinical isolates. Metabolites from MSU001 significantly inhibited growth of E. coli but not the mosquito gut symbionts Serratia marcescens and Asaia sp. W12. Insect-associated E. anophelis carried unique glycoside hydrolase (GH) and auxiliary activities (AAs) encoding genes distinct from those of clinical isolates, indicating their potential role in reshaping chitin structure and other components involved in larval development or formation of the peritrophic matrix. Like other Elizabethkingia, MSU001 also carried abundant genes encoding two-component system proteins (51), transcription factor proteins (188), and DNA-binding proteins (13). E. anophelis MSU001 contains a repertoire of antibiotic resistance genes and several virulence factors. Its potential for opportunistic infections in humans should be further evaluated prior to implementation as a paratransgenesis agent (by transgenesis of a symbiont of the vector).

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

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Elizabethkingia anophelis MSU001 Isolated from Anopheles stephensi: Molecular Characterization and Comparative Genome Analysis

Chen,

Pham,

Terrapon

et al. 2024

Microorganisms

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“…In a Medline/Pubmed searching the keywords ''Elizabethkingia anophelis pediatric infections''we found only 21 previously reported cases [5,7,[10][11][12][13][14][15][16][17][18][19]. Our case is the first pediatric E. anophelis infection described in Europe.…”

Section: Discussionmentioning

confidence: 90%

Central Line-Associated Bloodstream Infection Due to Elizabethkingia anophelis: Case Report and Literature Review on Pediatric Infections

Maraki,

Katzilakis,

Neonakis

et al. 2024

Preprint

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Elizabethkingia anophelis is an opportunistic pathogen causing lifethreatening infections in humans, particularly in immunocompromised patients, neonates and elderly. Infections caused by this species are exceptionally challenging to treat due to its multiresistance to antimicrobials. We report a case of central line-associated bloodstream infection by E. anophelis in a 2.5-year-old girl with acute lymphoblastic leukemia successfully treated with a combination of piperacillin/tazobactam and amikacin. The literature was also reviewed on paediatric infections caused by E. anophelis. Accurate identification with MALDI-TOF, or using molecular techniques, is crucial because of varying susceptibilities among species. Early, accurate diagnosis and prompt effective treatment optimize outcomes.

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“…Compared to S. maltophilia, reports of coinfection with E. anophelis among patients with COVID-19 are relatively scarce. However, the prevalence of E. anophelis might be underestimated because of the risk of inaccurate bacterial identification [56,57]. For example, Elizabethkingia spp.…”

Section: Key Pointsmentioning

confidence: 99%

Emerging infections in vulnerable hosts: Stenotrophomonas maltophilia and Elizabethkingia anophelis

Lee,

Hsueh

2023

Current Opinion in Infectious Diseases

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Purpose of review This systematic review aimed to explore the recent trends in the epidemiology, risk factors, and antimicrobial susceptibility of two emerging opportunistic pathogens, Stenotrophomonas maltophilia and Elizabethkingia anophelis. Recent findings Since 2020, numerous outbreaks of S. maltophilia and E. anophelis have been reported worldwide. Most of these outbreaks have been associated with healthcare facilities, although one outbreak caused by E. anophelis in France was considered a community-associated infection. In terms of antimicrobial susceptibility, trimethoprim/sulfamethoxazole (TMP-SMZ), levofloxacin, and minocycline have exhibited good efficacy against S. maltophilia. Additionally, cefiderocol and a combination of aztreonam and avibactam have shown promising results in in vitro susceptibility testing. For E. anophelis, there is currently no consensus on the optimal treatment. Although some studies have reported good efficacy with rifampin, TMP-SMZ, piperacillin/tazobactam, and cefoperazone/sulbactam, minocycline had the most favourable in vitro susceptibility rates. Cefiderocol may serve as an alternative due to its low minimum inhibitory concentration (MIC) against E. anophelis. The role of vancomycin in treatment is still uncertain, although several successful cases with vancomycin treatment, even with high MIC values, have been reported. Summary Immunocompromised patients are particularly vulnerable to infections caused by S. maltophilia and E. anophelis, but the optimal treatment strategy remains inconclusive. Further research is necessary to determine the most effective use of conventional and novel antimicrobial agents in combatting these multidrug-resistant pathogens.

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16s rRNA-based phylogenetic analyses of Elizabethkingia anophelis: Detection of Elizabethkingia anophelis, a rare infectious agent from blood and determination of antibiotic susceptibility in Turkey

Cited by 4 publications

References 12 publications

Elizabethkingia anophelis MSU001 Isolated from Anopheles stephensi: Molecular Characterization and Comparative Genome Analysis

Elizabethkingia anophelis MSU001 Isolated from Anopheles stephensi: Molecular Characterization and Comparative Genome Analysis

Central Line-Associated Bloodstream Infection Due to Elizabethkingia anophelis: Case Report and Literature Review on Pediatric Infections

Emerging infections in vulnerable hosts: Stenotrophomonas maltophilia and Elizabethkingia anophelis

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