16S rRNA Gene Diversity in the Salt Crust of Salar de Uyuni, Bolivia, the World’s Largest Salt Flat

Pecher, Wolf T.; Martínez, Fabiana Lilian; DasSarma, Priya; Guzmán, Daniel; DasSarma, Shiladitya

doi:10.1128/mra.00374-20

Cited by 8 publications

(9 citation statements)

References 17 publications

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“…The mines are located at an elevation of 1,230 m above sea level, where temperatures range from 10°C to 37°C. The samples, BOL5 (gray salt) (21°24′29.27″S, 64°07′55.55″W) and BOL6 (pink salt) (21°24′19.73″S, 64°07′51.52″W), were processed as previously described ( 12 , 14 , 15 ). Briefly, samples were collected using flame-sterilized tools and placed into sterile plastic bags.…”

Section: Announcementmentioning

confidence: 99%

16S rRNA Gene Diversity in Ancient Gray and Pink Salt from San Simón Salt Mines in Tarija, Bolivia

Pecher

Martínez

DasSarma

et al. 2020

Microbiol Resour Announc

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Section: Announcementmentioning

confidence: 99%

16S rRNA Gene Diversity in Ancient Gray and Pink Salt from San Simón Salt Mines in Tarija, Bolivia

Pecher

Martínez

DasSarma

et al. 2020

Microbiol Resour Announc

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“…These pigments are known to protect haloarchaeal cells from extremophilic conditions such as osmotic stress, damage from high ultraviolet light exposure and anoxic or anaerobic conditions [ 62 , 63 ]. They contribute also in the salt crystallization process [ 63 , 64 ]. Bacterioruberin pigment content in some saline ecosystems has been used to survey the haloarchaeal community in such ecosystems [ 65 ].…”

Section: Resultsmentioning

confidence: 99%

Assessment of 16S rRNA Gene-Based Phylogenetic Diversity of Archaeal Communities in Halite-Crystal Salts Processed from Natural Saharan Saline Systems of Southern Tunisia

Najjari

Stathopoulou

Elmnasri³

et al. 2021

Biology

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A thorough assessment of the phylogenetic diversity and community structure of halophilic archaea from three halite-crystal salts, processed from two separated saline systems of Southern Tunisia has been performed using culture dependent and independent methods targeting different regions of 16S rRNA gene sequences including DGGE, 16S rRNA clone libraries and Illumina Miseq sequencing. Two samples, CDR (red halite-crystal salts) and CDW (white halite-crystal salts), were collected from Chott-Eljerid and one sample CDZ (white halite-crystal salts) from Chott Douz. Fourteen isolates were identified as Halorubrum, Haloferax, Haloarcula, and Halogeometricum genera members. Culture-independent approach revealed a high diversity of archaeal members present in all samples, represented by the Euryarchaeal phylum and the dominance of the Halobacteria class. Nanohaloarchaea were also identified only in white halite samples based on metagenomic analysis. In fact, a total of 61 genera were identified with members of the Halorhabdus, Halonotius, Halorubrum, Haloarcula, and unclassified. Halobacteriaceae were shared among all samples. Unexpected diversity profiles between samples was observed where the red halite crust sample was considered as the most diverse one. The highest diversity was observed with Miseq approach, nevertheless, some genera were detected only with 16S rRNA clone libraries and cultured approaches.

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“…This statement is highly relevant to the hypersaline ecosystems broadly distributed around the world, from the Antarctic to the Himalayas, from Australia to the USA, from Africa to South America, and thus are much dissimilar in terms of salt concentration, chemical composition, and presence of additional stress conditions designated by geological attributes [5,[35][36][37]. Therefore, they are not only characterised by high-salt content but other environmental physicochemical extrema like high pressure and UV radiation, low oxygen concentration, hydrophobic conditions, extreme temperatures and pH, high concentrations of toxic compounds and heavy metals [27,36,[38][39][40][41][42].…”

Section: Biodiversity Of Hypersaline Environmentsmentioning

confidence: 99%

The Methods of Digging for “Gold” within the Salt: Characterization of Halophilic Prokaryotes and Identification of Their Valuable Biological Products Using Sequencing and Genome Mining Tools

Lach

Jecz

Strapagiel

et al. 2021

Genes

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Halophiles, the salt-loving organisms, have been investigated for at least a hundred years. They are found in all three domains of life, namely Archaea, Bacteria, and Eukarya, and occur in saline and hypersaline environments worldwide. They are already a valuable source of various biomolecules for biotechnological, pharmaceutical, cosmetological and industrial applications. In the present era of multidrug-resistant bacteria, cancer expansion, and extreme environmental pollution, the demand for new, effective compounds is higher and more urgent than ever before. Thus, the unique metabolism of halophilic microorganisms, their low nutritional requirements and their ability to adapt to harsh conditions (high salinity, high pressure and UV radiation, low oxygen concentration, hydrophobic conditions, extreme temperatures and pH, toxic compounds and heavy metals) make them promising candidates as a fruitful source of bioactive compounds. The main aim of this review is to highlight the nucleic acid sequencing experimental strategies used in halophile studies in concert with the presentation of recent examples of bioproducts and functions discovered in silico in the halophile’s genomes. We point out methodological gaps and solutions based on in silico methods that are helpful in the identification of valuable bioproducts synthesized by halophiles. We also show the potential of an increasing number of publicly available genomic and metagenomic data for halophilic organisms that can be analysed to identify such new bioproducts and their producers.

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16S rRNA Gene Diversity in the Salt Crust of Salar de Uyuni, Bolivia, the World’s Largest Salt Flat

Cited by 8 publications

References 17 publications

16S rRNA Gene Diversity in Ancient Gray and Pink Salt from San Simón Salt Mines in Tarija, Bolivia

16S rRNA Gene Diversity in Ancient Gray and Pink Salt from San Simón Salt Mines in Tarija, Bolivia

Assessment of 16S rRNA Gene-Based Phylogenetic Diversity of Archaeal Communities in Halite-Crystal Salts Processed from Natural Saharan Saline Systems of Southern Tunisia

The Methods of Digging for “Gold” within the Salt: Characterization of Halophilic Prokaryotes and Identification of Their Valuable Biological Products Using Sequencing and Genome Mining Tools

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