16S rRNA gene profiling of planktonic and biofilm microbial populations in the Gulf of Guinea using Illumina NGS

Celikkol-Aydin, Sukriye; Gaylarde, Christine C.; Lee, Tim; Melchers, Robert E.; Witt, Devin L.; Beech, Iwona B.

doi:10.1016/j.marenvres.2016.10.001

Cited by 36 publications

(24 citation statements)

References 46 publications

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“…Some differences, however, were also observed with Bacteroidetes distributions, for example, being high in seawater through to lowest in the tubercle inner layer in our study (Fig. 4), but the opposite being reported in (22).…”

Section: Discussioncontrasting

confidence: 74%

“…There were some similarities between our bacterial findings and a third MIC study (Zhang 2019), particularly with similar abundances of Proteobacteria and Bacteroidetes. In general, other studies have reported more Firmicutes (X. Li et al (21), S. Celikkol-Aydin et al (22) – mostly Defulfotomaculum ) and sometimes more Actinobacteria (22, 23), compared to our results, but the Proteobacteria and Bacteroidetes are abundant phyla in all these studies.…”

Section: Discussionsupporting

confidence: 53%

“…It has been suggested (26) that algae then cyanobacteria are primary terrestrial biofilm colonisers and data from S. Celikkol-Aydin et al (22) add credence to the notion they could be primary colonisers in marine metallic corrosion settings. We did not specifically explore algae, and our cyanobacteria were largely only in the seawater sample.…”

Section: Discussionmentioning

confidence: 93%

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Presence of orange tubercles does not always indicate accelerated low water corrosion

Phan

Wade

Blackall

2019

Preprint

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The rapid degradation of marine infrastructure at the low tide level due to 18 accelerated low water corrosion (ALWC) is a problem encountered worldwide. Despite 19 this, there is limited understanding of the microbial communities involved in this 20 process. We obtained samples of the orange-coloured tubercles commonly associated 21 with ALWC from two different types of steel sheet piling, located adjacent to each other 22 but with different levels of localised corrosion, at a seaside harbour. The microbial 23 communities from the outer and inner layers of the orange tubercles, and from adjacent 24 seawater, were studied by pure culture isolation and metabarcoding of the 16S rRNA 25 genes. A collection of 119 bacterial isolates was obtained from one orange tubercle 26 sample, using a range of media with anaerobic and aerobic conditions. The 27 metabarcoding results showed that sulfur and iron oxidisers were more abundant on the 28 outer section of the orange tubercles compared to the inner layers, where 29 Deltaproteobacteria (which includes many sulfate reducers) were more abundant. The 30 microbial communities varied significantly between the inner and outer layers of the 31 orange tubercles and also with the seawater, but overall did not differ significantly 32 between the two steel sheet types. Metallurgical analysis found differences in 33 composition, grain size, ferrite-pearlite ratio and the extent of inclusions present 34 between the two steel types investigated. 35 IMPORTANCEThe presence of orange tubercles on marine steel pilings is often used 36 as an indication that accelerated low water corrosion is taking place. We studied the 37 microbial communities in attached orange tubercles on two closely located sheet pilings 38 that were of different steel types. The attached orange tubercles were visually similar, 39 but the extent of underlying corrosion on the different steel surfaces were substantially 40 Phan et al., Applied and Environmental Microbiology 3 different. No clear difference was found between the microbial communities present on 41 the two different types of sheet piling. However, there were clear differences in the 42 microbial communities in the corrosion layers of tubercles, which were also different to 43 the microbes present in adjacent seawater. The overall results suggest that the 44 presence of orange tubercles, a single measurement of water quality, or the detection of 45 certain general types of microbes (e.g. sulfate reducing bacteria) should not be taken 46 alone as definitive indications of accelerated corrosion. 47 48 Microbiologically/microbially influenced corrosion (MIC) refers to the deterioration of 49 materials due to the presence of microbes and/or biofilms attached to the surface (1). A 50 special case of MIC is accelerated low water corrosion (ALWC), which is associated 51 with the rapid corrosion of metallic structures at the low tide water level (Fig. 1) (2). The 52 corrosion rates due to ALWC can be up to several mm/yr, compared to around 53 0.05 mm/yr which i...

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Section: Discussioncontrasting

confidence: 74%

Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 93%

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Presence of orange tubercles does not always indicate accelerated low water corrosion

Phan

Wade

Blackall

2019

Preprint

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“…This difference between the medium and the biofilm in terms of microbial flora might reflect a real dissociation between the composition of the medium and the composition of the biofilm at the surface of the material (Figure 3, Figure 4). Celikkol-Aydin et al [51] characterized a marine biofilm dominated by the phyla Cyanobacteria, Bacteroidetes and Proteobacteria while the seawater in which it was formed was dominated by the phyla firmicutes and proteobacteria [51]. In this study, the steel surface was a favourable environment for the enrichment of Cyanobacteria and Bacteroidetes.…”

Section: Heterogeneity Between the Planktonic Bacterial Populations (mentioning

confidence: 72%

Evaluation of microbial proliferation on cementitious materials exposed to biogas systems

Voegel

Durban

Bertron

et al. 2019

Environmental Technology

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Understanding the interactions between biofilm and cementitious materials in biogas production systems is an essential step toward the development of durable concrete for this expanding sector. Although the action of the liquid phase medium on the material has been the subject of several research studies, the possible impact of the material's properties on biofilm formation and composition has been little investigated, if at all. The aim of this paper is to evaluate the characteristics of the biofilm according to the surface properties of the materials. Four cementitious materials with different chemical and mineralogical compositions, and various topological surface characteristics (pastes of CEM I, CEM III/C and CAC, and CEM I paste treated with oxalic acid) were exposed to the liquid phase of a fermenting biowaste for 10 weeks. The steps of biofilm formation were observed using SEM. Even though all the cementitious material surfaces were intensely colonized at the end of the experiments, the establishment of the biofilm seems to have been delayed on the oxalate-treated CEM I and on CAC coupons. Roughness and surface pH effects were not of prime importance for the biofilm development. The analysis of bacterial population diversity using 16S rDNA sequencing showed a less diversified microbial flora in the biofilm than in the reaction medium.

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“…At the same time, the development of biological characterization tools in the past few years has provided important insights into specific marine bacterial groups and their significance in biocorrosion under controlled laboratory conditions [32][33][34][35]. More recent studies based on complete community approaches have finally revealed the complexity of real microbial systems and paved the way for a more systematic understanding of the biocorrosion phenomenon [36][37][38][39][40]. The current understanding is that biocorrosion associated with biofilm formation and growth in natural environments is a complex process; the nonlinearity of this process is determined by numerous factors, of both the abiotic and biotic types.…”

Section: Introductionmentioning

confidence: 99%

Effect of Tidal Cycles on Bacterial Biofilm Formation and Biocorrosion of Stainless Steel AISI 316L

Daille

Aguirre

Fischer

et al. 2020

JMSE

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The effects of tidal cycles associated with the water level on the biocorrosion of stainless steel AISI 316L were studied. Steel coupons were exposed to different conditions of immersion in mesocosms fed by fresh seawater either continuously or in accordance with the periodicity of natural tides. After 5 and 15 weeks, all coupons were found to have undergone ennoblement associated with the formation of a biofilm. Analysis of the composition of the bacterial community using denaturing gradient gel electrophoresis (DGGE) revealed differences in the biological succession. After 15 weeks, exposure to the simulated tidal conditions resulted in biofilms with lesser bacterial richness; the corresponding rate of corrosion, as determined by weight loss, was about 40 times lower compared to the case for the continuous exposure to seawater. Phylogenetic analysis of selected DGGE bands and the inspection of biofilm morphologies revealed that the faster rate of corrosion was associated with the presence of iron-oxidizing Zetaproteobacteria and eukaryotic photosynthetic microorganisms. On the other hand, intermittent exposure to seawater resulted in the succession of microorganisms resistant to the stress associated with sudden environmental changes, which was associated with a low rate of corrosion.

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16S rRNA gene profiling of planktonic and biofilm microbial populations in the Gulf of Guinea using Illumina NGS

Cited by 36 publications

References 46 publications

Presence of orange tubercles does not always indicate accelerated low water corrosion

Presence of orange tubercles does not always indicate accelerated low water corrosion

Evaluation of microbial proliferation on cementitious materials exposed to biogas systems

Effect of Tidal Cycles on Bacterial Biofilm Formation and Biocorrosion of Stainless Steel AISI 316L

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