16S rRNA is a better choice than COI for DNA barcoding hydrozoans in the coastal waters of China

Zheng, Lianming; He, Jinru; Lin, Yan; Cao, Wenqing; Zhang, Wenjing

doi:10.1007/s13131-014-0415-8

Cited by 77 publications

(57 citation statements)

References 65 publications

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“…Species grouped in distinct clusters with high support in the 16S tree. 16S has been shown to be a powerful phylogenetic marker in delineating species boundaries for marine hydroids, and has also been demonstrated to be more successful than the mitochondrially encoded cytochrome c oxidase I (COI) gene in extrapolating phylogenetic relationships of hydroids at the generic level, and in some cases at the family level (Miglietta et al 2007(Miglietta et al , 2009Moura et al 2008Moura et al , 2011Peña Cantero et al 2010;Zheng et al 2014). Our findings are also consistent with previous work supporting the nonmonophyly of the Anthoathecata and Filifera (Cartwright et al 2008).…”

Section: Phylogenetic Analysissupporting

confidence: 89%

“…In terms of seasonality, active colonies have been observed and photographed in waters of BC during the months of January (1974, 1976, 2014), March (2014), April (1978, 2014, May (2014), June (2014), July (2014), August (2014), September ( , 2014( ), and November (1971. During each of those months, gonophores were discernible on at least some of the hydranths.…”

Section: Ecologymentioning

confidence: 99%

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Similiclava nivea(Cnidaria: Hydrozoa: Similiclavidae): a new family, genus and species of athecate hydroid from the Pacific coast of North America

Calder

Choong

McDaniel³

2015

Journal of Natural History

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The hydroid Similiclava nivea, gen. nov., sp. nov., is described from colonies collected in nearshore waters of southern British Columbia, Canada. It has been observed by divers, and recorded as Clava sp., several hundred times at locations between southeast Alaska and southern Oregon, USA. While resembling the hydractiniid Clava multicornis, tentacles of S. nivea are arranged in two close whorls in extended hydranths rather than being scattered over the distal end of the hydranth. Moreover, the hypostome is dome-shaped to flattened instead of being proboscidiform. Similiclava nivea also resembles species of the hydractiniid genera Stylactaria, Hydractinia and Podocoryna, but differs in being monomorphic, in lacking spines on the hydrorhiza and in having numerous (as many as 50 or more) sporosacs borne in a broad whorl below the tentacles on normal hydranths. Molecular analysis using the mitochondrial 16S gene sequences reveals that S. nivea is closer to Clathrozoellidae than to Hydractiniidae, although it is clearly distinct from both. A new family, Similiclavidae, is established to accommodate the species. Hydroids of S. nivea were observed at relatively shallow depths (10-30 m) on rocks and epibiota attached to rocky substrates. Colonies are conspicuous because of the relatively large surface area they occupy (up to 20 cm across, and in aggregations often exceeding 100 or more hydranths) together with the height (about 15 mm high when extended) and striking white colour of living hydranths. The cnidome consists of desmonemes, microbasic euryteles and microbasic mastigophores. A substrate generalist observed on rocks, algae, sponges, barnacles, bryozoans and other hydroids in cold-temperate waters, S. nivea is most prevalent in shallow subtidal areas swept by tidal currents.http://zoobank.org/urn:lsid:zoobank.org:pub:2691E02E-7E14-4820-8D91-41D9E7E8BF62

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Section: Phylogenetic Analysissupporting

confidence: 89%

Section: Ecologymentioning

confidence: 99%

Similiclava nivea(Cnidaria: Hydrozoa: Similiclavidae): a new family, genus and species of athecate hydroid from the Pacific coast of North America

Calder

Choong

McDaniel³

2015

Journal of Natural History

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“…This manuscript version is made available under the Elsevier user license http://www.elsevier.com/open-access/userlicense/1.0/with the ability to break this biological dogma by reversing its ontogeny under stressful conditions (Piraino et al, 2004;Schmich et al, 2007), which is manifested by back transformation of either the newly liberated, immature medusa, or adult specimen with ripened gonads (Piraino et al, 1996) into the preceding stage, the polyp (Bavestrello et al 1992). This remarkable feature and its high reproducibility has generated broad interest in this species as a potential biological model for research on ageing and the molecular mechanisms of cell differentiation and transdifferentiation (Bosch et al, 2014;Petralia et al, 2014; Sanchez-Alvarado and Yamanaka, 2014).Although several genes have been sequenced in T. dohrnii for phylogenetic reconstruction (Miglietta et al, 2007;Miglietta and Lessios, 2009;Quiquand et al, 2009, Devarapalli et al, 2014Zheng et al, 2014), the complete mitochondrial genome has not yet been reported. Here we present the complete sequence of the mitochondrial genome of T.dohrnii, including analysis of its structure and organization.…”

mentioning

confidence: 99%

Complete mitochondrial genome and evolutionary analysis of Turritopsis dohrnii, the “immortal” jellyfish with a reversible life-cycle

Lisenkova

Grigorenko

Tyazhelova

et al. 2017

Molecular Phylogenetics and Evolution

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Turritopsis dohrnii (Cnidaria, Hydrozoa, Hydroidolina, Anthoathecata) is the only known metazoan that is capable of reversing its life cycle via morph rejuvenation from the adult medusa stage to the juvenile polyp stage. Here, we present a complete mitochondrial (mt) genome sequence of T. dohrnii, which harbors genes for 13 proteins, two transfer RNAs, and two ribosomal RNAs. The T. dohrnii mt genome is characterized by typical features of species in the Hydroidolina subclass, such as a high A+T content (71.5%), reversed transcriptional orientation for the large rRNA subunit gene, and paucity of CGN codons. An incomplete complementary duplicate of the cox1 gene was found at the 5' end of the T. dohrnii mt chromosome, as were variable repeat regions flanking the chromosome. We identified species-specific variations (nad5, nad6, cob, and cox1 genes) and putative selective constraints (atp8, nad1, nad2, and nad5 genes) in the mt genes of T. dohrnii, and predicted alterations in tertiary structures of respiratory chain proteins (NADH4, NADH5, and COX1 proteins) of T. dohrnii. Based on comparative analyses of available hydrozoan mt genomes, we also determined the taxonomic relationships of T. dohrnii, recovering Filifera IV as a paraphyletic taxon, and assessed intraspecific diversity of various Hydrozoa species.

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“…We employed two mitochondrial DNA (mtDNA) markers, the cytochrome c oxidase subunit 1 (COI) gene and the large subunit ribosomal RNA (rRNA) gene 16S. The slow evolutionary rates of COI and 16S rDNA in some hydro-zoans allow them to be useful markers (McFadden et al 2011;Zheng et al 2014). The COI is known as the main metazoan barcoding marker, although in hydrozoans the COI gen has scarcely been employed because of the difficulties involved in amplifying DNA fragments using standard primers (Cantero et al 2010;Schuchert 2010).…”

Section: Introductionmentioning

confidence: 99%

“…It is considered to be a gene that is much easier to amplify and is more available in the GenBank database (Cantero et al 2010;Moura et al 2011;Laakmann and Holst 2014;Zheng et al 2014).…”

Section: Introductionmentioning

confidence: 99%

First record of Aequorea macrodactyla (Cnidaria, Hydrozoa) from the Israeli coast of the eastern Mediterranean Sea, an alien species indicating invasive pathways

Mizrahi¹,

Shemesh²,

Ofwegen³

et al. 2015

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The species of Aequorea attract much scientific interest as they contain the unique Green Fluorescent Protein (GFP). In this work we describe for the first time the discovery of a hydrozoan jellyfish belonging to the genus Aequorea from the Israeli eastern Mediterranean that contains and exhibits fluorescent protein. Finding Aequorea macrodactyla (Brandt, 1835) in the eastern Mediterranean indicates that changes are occurring in the gelatinous fauna of this area. This hydromedusa is known in the seas adjoining the Mediterranean though most of its records are more than four decades old. We examined and identified the newly discovered Israeli Aequorea species by combining two phylogenetic systems, traditional morphological phylogeny and molecular phylogenetics. The molecular identification determined that the species is A. macrodactyla but with minor genetic differences in the mtDNA 16S gene marker. A 1% difference between the Israeli and the Japanese A. macrodactyla was demonstrated, which suggests that the genetic difference between the Israeli and the Japanese population is small but existent. Invasive pathways for this jellyfish were examined by phylogenetic and taxonomic relationships with similar Cnidaria. The results indicate introduction from the Indo-Pacific as invasive pathway, probably by human transportation, and the discovery of A. macrodactyla in the eastern Mediterranean Sea could be interpreted as part of the changes in marine biota as a result of cumulative effects of anthropogenic and global changes that affect the eastern Mediterranean basin. Gur A. Mizrahi et al. / NeoBiota 26: 55-70 (2015) 56 NeoBiota

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16S rRNA is a better choice than COI for DNA barcoding hydrozoans in the coastal waters of China

Cited by 77 publications

References 65 publications

Similiclava nivea(Cnidaria: Hydrozoa: Similiclavidae): a new family, genus and species of athecate hydroid from the Pacific coast of North America

Similiclava nivea(Cnidaria: Hydrozoa: Similiclavidae): a new family, genus and species of athecate hydroid from the Pacific coast of North America

Complete mitochondrial genome and evolutionary analysis of Turritopsis dohrnii, the “immortal” jellyfish with a reversible life-cycle

First record of Aequorea macrodactyla (Cnidaria, Hydrozoa) from the Israeli coast of the eastern Mediterranean Sea, an alien species indicating invasive pathways

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