[17] Methods for studying biofilms produced by staphylococcus epidermidis

Deighton, Margaret; Capstick, Jill; Domalewski, E; Nguyen, Thuong Van

doi:10.1016/s0076-6879(01)36589-8

Cited by 54 publications

(52 citation statements)

References 73 publications

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“…This is consistent with previous studies by Deighton et al (2001), and also confirms that S. epidermidis RP62A (ATCC 35984) is a strong biofilm producer. The crystal violet stained cell optical density confirmed the biofilm thickness that is expressed as an index of adherence to the surface of the plate and not the presence of EPS.…”

Section: Toluidine Blue Stainingsupporting

confidence: 93%

“…Biofilm growth in a microtitre plate S. epidermidis biofilms were grown according to the procedure described by Deighton et al (2001) with slight modifications. Approximately 50µl of fresh TSB culture containing 3.0x10 6 cfu/ml was inoculated into 8 wells of a flat bottomed microtitre plate (Greiner bio-one, Germany) and incubated for 24hrs at 37 O C. After incubation, excess medium containing planktonic cells were removed and the plate washed with 1xTris-buffered saline (TBS, pH 7.6) for three times using ELISA washer (bioTek, USA) and dried for at least 2hrs at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…epidermidis biofilms were grown according to the procedure described by Deighton et al (2001) with slight modifications and incubated for 30 minutes, 2hrs, 4hrs and 24hrs at 37 O C respectively. The microtitre plates were stained using 0.4% (w/v) ammonium crystal violet stain and incubated for 10 minutes at room temperature to evaluate the presence of bacterial cells in the biofilms.…”

Section: Crystal Violet Staining and Time-course Analysismentioning

confidence: 99%

See 2 more Smart Citations

Modification of Glycan Expression in Staphylococcus epidermidis to Prevent Biofilm Formation

Solomon¹,

Etim²,

Ekong³

2017

Int.J.Curr.Microbiol.App.Sci

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Section: Toluidine Blue Stainingsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Crystal Violet Staining and Time-course Analysismentioning

confidence: 99%

See 1 more Smart Citation

Modification of Glycan Expression in Staphylococcus epidermidis to Prevent Biofilm Formation

Solomon¹,

Etim²,

Ekong³

2017

Int.J.Curr.Microbiol.App.Sci

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“…Bacterial biofilm culture was set up using a modification of a previously established method. 31 Briefly, an overnight bacterial culture grown in nutrient broth was diluted 1:100 into biofilmspecific growth media, including tryptic soya broth for S. epidermidis (Oxoid) and Luria-Bertani broth for P. aeruginosa. One hundred microliters of the diluted bacterial suspensions was pipetted into a well in 96-well flat-bottom polystyrene microplates that had received different coating treatments and were incubated for 24 h at 37°C with gentle agitation (75 r.p.m.).…”

Section: Biofilm Cultivation and Quantitative Determinationmentioning

confidence: 99%

Prebiotic-chemistry inspired polymer coatings for biomedical and material science applications

et al. 2015

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In the field of prebiotic chemistry, hydrogen cyanide (HCN)-derived polymers have been studied for many years as a possible source of the precursors that provide the building blocks for proteins as well as nucleic acids, and they have also been associated with the origin of life. The HCN trimer, aminomalononitrile (AMN), polymerizes to give a brown complex nitrogenous polymer. We report the one-step polymerization-deposition of AMN as a simple generic surface-coating method and as an application of prebiotic chemical research to material science. We found that this polymerization, carried out in buffered aqueous solutions, can be used to coat a wide range of organic and inorganic substrate materials. The robust, non-cytotoxic coatings also provide for excellent cell attachment, suggesting potential biomedical applications. Furthermore, the coating chemistry allows for the immobilization of other compounds, including metals, both during coating formation or by performing secondary immobilization reactions.

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“…(Deighton et al, 2001;Mathur et al, 2006;Agarwal et al, 2011). Nevertheless, the microtiter plate and Congo red agar method remains among the most frequently used assays for investigation of biofilm (Vasudevan et al, 2003;Knobloch et al, 2002).…”

Section: Staphylococcus Aureus For Biofilm Formation Abilitymentioning

confidence: 99%

Genotypic and Phenotypic Characterization of Clinical Isolates of Staphylococcus Aureus for Biofilm Formation Ability

Goyal¹

2014

Adv. Anim. Vet. Sci.

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The objective of the study was to characterize Staphylococcus aureus (S. aureus) isolated from human and animal clinical cases for their biofilm formation ability by genotypic and phenotypic methods. A total of 130 S. aureus strains isolated from human wound (n = 20), animal wound (n = 70) and animal mastitis (n = 40) cases were subjected to screening for 3 different biofilm associated genes (bap, icaA and icaD) and for phenotypic assessment for biofilm formation using Congo red agar, modified Congo red agar and microtitre plate assay. PCR assays were standardized for the detection of bap, icaA and icaD genes. The results indicated that icaA gene was present in 51.15% of the isolates and bap gene was present in 8.46% isolates. None of the isolates were positive for icaD gene. Human isolates (65%) had higher occurrence of icaA gene in comparison to animal isolates (49.09%). Dog wound isolates had higher occurrence of bap gene. Of the 3 methods used for phenotypic expression of biofilm by S. aureus isolates modified Congo red agar method showed 86.92% isolates to be positive, whereas by Congo red agar method only 63.07% S. aureus were found to be biofilm producer. Microtitre plate assay showed 75.38% S. aureus isolates to be biofilm producers. A good correlation was observed between genotypic and phenotypic biofilm formation ability of the isolates. Bap gene contained isolates showed higher biofilm producing ability compare to icaA gene harbored isolates.All copyrights reserved to Nexus® academic publishers

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[17] Methods for studying biofilms produced by staphylococcus epidermidis

Cited by 54 publications

References 73 publications

Modification of Glycan Expression in Staphylococcus epidermidis to Prevent Biofilm Formation

Modification of Glycan Expression in Staphylococcus epidermidis to Prevent Biofilm Formation

Prebiotic-chemistry inspired polymer coatings for biomedical and material science applications

Genotypic and Phenotypic Characterization of Clinical Isolates of Staphylococcus Aureus for Biofilm Formation Ability

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