[17] Polyethylene glycol and electric field-mediated cell fusion for formation of hybridomas

Comparison of PEG-Induced and Electric Field-Mediated Cell Fusion in the Generation of Monoclonal Antibodies against a Variety of Soluble and Cellular Antigens

“…Furthermore, there exists a significant correlation between both methods in individual experiments [10]. In a considerable number of cases a conventional PEG fusion was performed in parallel.…”

“…Our first successful experiment was performed in April 1984 [5]. We were surprised by the obvious advantage of electrofusion over polyethylene glycol (PEG) mediated fusions, which was confirmed in later experiments by ourselves [6,10] as well as by others [7][8][9]· This chapter is intended to outline our version of electrofusion as with this instrument in a number of model systems, and our long-term experience with cell cultivation and the hybridoma technology in general.…”

Development and characterization of monoclonal antibodies for the immunohistochemical detection of glycodelin A in decidual, endometrial and gynaecological tumour tissues

“…Eight months later, a boost with 100 µg of purified glycodelin A in PBS was given intraperitoneally. After 3 days, spleen cells were prepared and fused with X63‐Ag8.653 cells according to standard techniques 16,17 using polyethylene glycol with a few exceptions: azaserine was employed instead of aminopterin as selection medium and early hybridoma growth was supported by mouse peritoneal feeder cells. Supernatants were examined in ELISA assays with GdA coated to microtitre plates.…”

A novel series of anti-human glycophorin A (CD235a) antibodies defining five extra- and intracellular epitopes

“…Hybridomas were generated according to techniques described in detail elsewhere [18]. Mice used for immunization were Balb/c (in most cases), C3H, or F1 hybrids of C57BL × XVII (Bln) mice.…”