1731, A New Retrotransposon with Hormone Modulated Expression

Peronnet, Frédérique; Becker, Jacqueline; Lescault, A.; d'Auriol, L; Galibert, Francis; Best‐Belpomme, Martin

doi:10.1007/978-3-642-73626-1_11

Cited by 8 publications

(8 citation statements)

References 34 publications

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“…One important clue discovered toward the end of the 1980s was the observation that some TEs contain internal sequences similar to those found in murine mammary tumor virus LTRs, which are known to play a role in the regulation of the provirus by steroid hormones (Peronnet et al 1986;Ziarczyk and Best-Belpomme 1991), and element 412 was found to contain a 20-HD ecdysoneresponsive repetitive sequence (Micard et al 1988). In addition to such hormone-sensitive sequences, the presence of sequences homologous to heat-shock consensus sequences suggested the possibility that TEs could be sensitive to their environment.…”

Section: Transposable Elements As Components Of Genetic Diversitymentioning

confidence: 99%

A Brief History of the Status of Transposable Elements: From Junk DNA to Major Players in Evolution

2010

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The idea that some genetic factors are able to move around chromosomes emerged more than 60 years ago when Barbara McClintock first suggested that such elements existed and had a major role in controlling gene expression and that they also have had a major influence in reshaping genomes in evolution. It was many years, however, before the accumulation of data and theories showed that this latter revolutionary idea was correct although, understandably, it fell far short of our present view of the significant influence of what are now known as ''transposable elements'' in evolution. In this article, I summarize the main events that influenced my thinking about transposable elements as a young scientist and the influence and role of these specific genomic elements in evolution over subsequent years. Today, we recognize that the findings about genomic changes affected by transposable elements have considerably altered our view of the ways in which genomes evolve and work.

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Section: Transposable Elements As Components Of Genetic Diversitymentioning

confidence: 99%

A Brief History of the Status of Transposable Elements: From Junk DNA to Major Players in Evolution

2010

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“…flea: a 4.9 kb Pvul fragment (Kidd & Young, 1986). 1731: a 3 kb BamHI-Sall fragment from plasmid pFP5c (Peronnet et al, 1986). 297: a 3.5 kb Xbal-Xhol fragment from plasmid cDM4006 (Mossie, Young & Varmus, 1985).…”

Section: Probes For In Situ Hybridizationmentioning

confidence: 99%

Spatially regulated expression of retrovirus-like transposons duringDrosophila melanogasterembryogenesis

Ding¹,

Lipshitz²

1994

Genet. Res.

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Over twenty distinct families of long terminal direct repeat (LTR)-containing retrotransposons have been identified in Drosophila melanogaster. While there have been extensive analyses of retrotransposon transcription in cultured cells, there have been few studies of the spatial expression of retrotransposons during normal development. Here we report a detailed analysis of the spatial expression patterns of fifteen families of retrotransposons during Drosophila melanogaster embryogenesis (17.6, 297, 412, 1731, 3S18, blood, copia, gypsy, HMS Beagle, Kermit/flea, mdgl, mdg3, opus, roo/B104 and springer). In each case, analyses were carried out in from two to four wild-type strains. Since the chromosomal insertion sites of any particular family of retrotransposons vary widely among wild-type strains, a spatial expression pattern that is conserved among strains is likely to have been generated through interaction of host transcription factors with cw-regulatory elements resident in the retrotransposons themselves. All fifteen families of retrotransposons showed conserved patterns of spatially and temporally regulated expression during embryogenesis. These results suggest that all families of retrotransposons carry cw-acting elements that control their spatial and temporal expression patterns. Thus, transposition of a retrotransposon into or near a particular host gene -possibly followed by an excision event leaving behind the retrotransposon's cw-regulatory sequences -might impose novel developmental control on such a host gene. Such a mechanism would serve to confer evolutionarily significant alterations in the spatio-temporal control of gene expression.

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“…1), resulting in RNA that serves as a template for both translation and reverse transcription (Peronnet et al, 1986). In a typical life cycle of the retrotransposon, Gag and Gag-Pol proteins, together with retroelement mRNAs, are able to form virus-like particles (VLPs), which are essential transposition intermediates since reverse transcription of mRNA takes place in VLPs.…”

confidence: 99%

Virus-like particle formation in <i>Drosophila melanogaster</i> germ cells suggests a complex translational regulation of the retrotransposon cycle and new mechanisms inhibiting transposition

Rachidi

Lopes²,

Bénichou³

et al. 2005

Cytogenet Genome Res

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Transposition of 1731, a Drosophila melanogaster LTR retrotransposon, was investigated in reproductive organs by RNA, protein and VLP distribution during its life cycle. We detected 1731 transcription in oogonia but not in spermatogonia; in all cells during oogenesis but only in primary spermatocytes; and in ovarian cytoplasm but both in nuclei and cytoplasm of primary spermatocytes. By confocal scanning, we showed that whereas Gag protein appeared in all cytoplasms during oogenesis, in testes Gag detection began in late premeiotic primary spermatocytes and increased in elongating spermatids suggesting distinct mechanisms of 1731 transcription and translation regulation. By electron microscopy, we did not detect 1731 VLPs in ovaries, suggesting a complex post-translational control blocking VLP assembly and transposition. Interestingly, in testes we discovered VLP aggregates in cystic cytoplasm of maturing partially individualized spermatids. In testes, we observed two delays in 1731 product expressions, suggesting a complex temporal control mechanism. Transcriptional/translational delay may be determined by accumulation of 1731 RNAs in primary spermatocyte nuclei. Translational/VLP assembly delay may be determined by post-transductional mechanisms controlling +1 frameshift and Pol-protein degradation. Our results indicated two differential mechanisms inhibiting 1731 transposition in Drosophila melanogaster ovaries and testes. In addition, we proposed a new mechanism for transposition control at the cell cycle level.

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1731, A New Retrotransposon with Hormone Modulated Expression

Cited by 8 publications

References 34 publications

A Brief History of the Status of Transposable Elements: From Junk DNA to Major Players in Evolution

A Brief History of the Status of Transposable Elements: From Junk DNA to Major Players in Evolution

Spatially regulated expression of retrovirus-like transposons duringDrosophila melanogasterembryogenesis

Virus-like particle formation in <i>Drosophila melanogaster</i> germ cells suggests a complex translational regulation of the retrotransposon cycle and new mechanisms inhibiting transposition

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