174 Secretome Analysis of Mesenchymal Stem Cells From Human Umbilical Cord Strome During the Chondrogenesis

Arufe, M.C.; Fuente, A. De la; Mateos, J.; Fernandez, Paula Gonzalez; Rendal, E.; Diaz, Silvina Laura; Fuentes, I.M.; Toro, F.J. De; Blanco, F.J.

doi:10.1016/s1063-4584(10)60201-4

Cited by 2 publications

(1 citation statement)

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“…MSCs were isolated from human umbilical stromal tissue using a protocol developed by our group [3]. Briefly, the isolation was performed through explants after three short incubations with a cocktail containing 1.2 U/mL dispase and 112 U/mL type I collagenase (all from Sigma-Aldrich).…”

Section: Isolation and Characterization Of Mscmentioning

confidence: 99%

Effect of miR-21 in mesenchymal stem cells-derived extracellular vesicles behavior

Morente-López,

Mato-Basalo,

Lucio-Gallego

et al. 2023

Stem Cell Res Ther

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Background A challenging new branch of research related to aging-associated diseases is the identification of miRNAs capable of modulating the senescence-associated secretory phenotype (SASP) which characterizes senescent cells and contributes to driving inflammation. Methods Mesenchymal stem cells (MSC) from human umbilical cord stroma were stable modified using lentivirus transduction to inhibit miR-21-5p and shotgun proteomic analysis was performed in the MSC-derived extracellular vesicles (EV) to check the effect of miR-21 inhibition in their protein cargo. Besides, we studied the paracrine effect of those modified extracellular vesicles and also their effect on SASP. Results Syndecan-1 (SDC1) was the most decreased protein in MSC-miR21−-derived EV, and it was involved in inflammation and EV production. MSC-miR21−-derived EV were found to produce a statistically significant inhibitory effect on SASP and inflammaging markers expression in receptor cells, and in the opposite way, these receptor cells increased their SASP and inflammaging expression statistically significantly when treated with MSC-miR-21+-derived EV. Conclusion This work demonstrates the importance of miR-21 in inflammaging and its role in SASP through SDC1. Graphical abstract

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Section: Isolation and Characterization Of Mscmentioning

confidence: 99%

Effect of miR-21 in mesenchymal stem cells-derived extracellular vesicles behavior

Morente-López,

Mato-Basalo,

Lucio-Gallego

et al. 2023

Stem Cell Res Ther

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Excellent Prognosis in a Subset of Patients with Ewing Sarcoma Identified at Diagnosis by CD56 Using Flow Cytometry

Ash

Luria

Cohen

et al. 2011

Clinical Cancer Research

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Purpose: Ewing sarcoma (ES) is considered a systemic disease with the majority of patients harboring micrometastases at diagnosis. Multiparameter flow cytometry (MPFC) was used to detect ES cells in bone marrow (BM) of ES patients at diagnosis and to evaluate the prognostic significance of CD56 expression in BM samples.Experimental Design: BM samples from 46 ES patients, 6 tumor aspirates, 2 ES cell lines, and 10 control BM samples were analyzed by MPFC. ES cells were identified by the combination of CD45À/CD90þ/ CD99þ. CD56 was evaluated on these cells by a cutoff of 22%.Results: BM samples obtained from all patients at diagnosis were found to be positive for micrometastatic tumor cells assessed by CD99þ/CD90þ/CD45À expression. A total of 60% of the BM samples harbored high CD56 expression. There was a highly significant correlation between CD56 expression and progression-free survival (PFS; 69% in low/negative expression versus 30% in high expression groups, P ¼ 0.024). In patients with localized nonpelvic disease, those expressing low/negative CD56 had 100% PFS versus 40% in the high expressing group (P ¼ 0.02).

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174 Secretome Analysis of Mesenchymal Stem Cells From Human Umbilical Cord Strome During the Chondrogenesis

Cited by 2 publications

References 0 publications

Effect of miR-21 in mesenchymal stem cells-derived extracellular vesicles behavior

Effect of miR-21 in mesenchymal stem cells-derived extracellular vesicles behavior

Excellent Prognosis in a Subset of Patients with Ewing Sarcoma Identified at Diagnosis by CD56 Using Flow Cytometry

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