17α-Ethynylestradiol biodegradation in different river-based groundwater recharge modes with reclaimed water and degradation-associated community structure of bacteria and archaea

Ma, Wing-Kin; Sun, Jiaji; Li, Yangyao; Lun, Xiaoxiu; Shan, Dan; Nie, Chao; Liu, Miaomiao

doi:10.1016/j.jes.2016.11.022

Cited by 32 publications

(6 citation statements)

References 34 publications

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“…Studies have shown that E2 is not a familiar metabolite of E3 biodegradation since E2 can be irreversibly converted to E3 (Lappano et al, 2010). Moreover, E3 has been proved to be degraded through 16α-OH-E1 (Ke et al, 2007), or be cleaved at ring D directly (Ma et al, 2018) rather than ring A/B as normally described; thus, it is rational that E2-degrading genes have no response to E3 induction.…”

Section: Discussionmentioning

confidence: 98%

Identification of novel catabolic genes involved in 17β‐estradiol degradation by Novosphingobium sp. ES2‐1

Sun

Yan

et al. 2021

Environmental Microbiology

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Summary Novosphingobium sp. ES2‐1 is an efficient 17β‐estradiol (E2)‐degrading bacterium, which can convert E2 to estrone (E1), then to 4‐hydroxyestrone (4‐OH‐E1) for subsequent oxidative cracking. In this study, the molecular bases for this process were elucidated. Two novel monooxygenase systems EstP and EstO were shown to catalyse the oxygenation of E1 and 4‐OH‐E1, respectively. EstP was a three‐component cytochrome P450 monooxygenase system consisting of EstP1 (P450 monooxygenase), EstP2 (ferredoxin) and EstP3 (ferredoxin reductase). Ultraperformance liquid chromatography‐high resolution mass spectrometry (UPLC‐HRMS) analysis revealed that EstP catalysed the 4‐hydroxylation of E1 to produce 4‐OH‐E1. The resultant 4‐OH‐E1 was further oxidized by a two‐component monooxygenase system EstO consisting of EstO1 (flavin‐dependent monooxygenases) and EstO2 (flavin reductase). UPLC‐HRMS combined with 1H‐nuclear magnetic resonance analysis demonstrated that EstO catalysed the breakage of C9‐C10 to yield a ring B‐cleavage product. In addition, the oxygenase component genes estP1 and estO1 exhibited contrary inductive behaviours when exposed to different steroids, suggesting that EstP1‐mediated 4‐hydroxylation was E2‐specific, whereas EstO1‐mediated monooxygenation might be involved in the degradation of testosterone, androstenedione, progesterone and pregnenolone. This also implied that the mechanisms of the catabolism of different steroids by the same microorganism might be partially interlinked.

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Section: Discussionmentioning

confidence: 98%

Identification of novel catabolic genes involved in 17β‐estradiol degradation by Novosphingobium sp. ES2‐1

Sun

Yan

et al. 2021

Environmental Microbiology

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“…Water 2019, 11,2639 8 of 11 C-H bond or addition to the C=C bond and C=O bond, which are conjugated with aromatic rings [38]. After HO· exposure, precursors that carry electron-donating groups generated products that were much more reactive toward electrophilic halogenation [39], leading to more DBP formation.…”

Section: Dbp Formationmentioning

confidence: 99%

“…In past decades, the widespread occurrence of micro-organic pollutants (MPs) has been documented in the aquatic environment, such as endocrine disrupting compounds (EDCs) and pharmaceutical and personal care products (PPCPs) [7][8][9][10][11]. The presence of EDCs and PPCPs in the aquatic environment has received great attention because of their potential adverse effects on aquatic ecosystems and human health [12,13].…”

Section: Introductionmentioning

confidence: 99%

Degradation of Micropollutants by UV–Chlorine Treatment in Reclaimed Water: pH Effects, Formation of Disinfectant Byproducts, and Toxicity Assay

Wang

Ying

Минг

et al. 2019

Water

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The utilization of reclaimed water is a reliable and sustainable approach to enhance water supply in water-deficient cities. However, the presence of micro-organic pollutants (MPs) in reclaimed water has potential adverse effects on aquatic ecosystems and human health. In this study, we investigated the occurrence of 12 target MPs in the influent and reclaimed water collected from a local wastewater treatment plant, and the ultraviolet (UV)-chlorine process was applied to analyze its ability to remove MPs. The results showed that all 12 MPs were detected in both the influent and the reclaimed water, with the concentrations ranging from 25.5 to 238 ng/L and 8.6 to 42.5 ng/L, respectively. Over 52% of all the target MPs were readily degraded by the UV-chlorine process, and the removal efficiency was 7.7% to 64.2% higher than the corresponding removal efficiency by chlorination or UV irradiation only. The degradation efficiency increased with the increasing initial chlorine concentration. The pH value had a slight influence on the MP degradation and exhibited different trends for different MPs. The formation of known disinfectant byproducts (DBPs) during the UV-chlorine process was 33.8% to 68.4% of that in the chlorination process, but the DBPs' formation potentials were 1.3 to 2.2 times higher. The toxicity assay indicated that UV-chlorine can effectively reduce the toxicity of reclaimed water.

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“…These figures are increasing as a consequence of government policies [ 2 , 3 ]. The safety of WWTP effluent has attracted scrutiny, even after it has met the criteria for discharge or reuse [ 4 , 5 , 6 ]. However, there are still persistent residual chemicals, such as endocrine-disrupting compounds (EDCs), pesticides, and pharmaceutical and personal care products [ 5 , 7 , 8 ], which are characterized by low concentrations (ng/L), high variety, and complicated physicochemical properties [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Adsorption of Trace Estrogens in Ultrapure and Wastewater Treatment Plant Effluent by Magnetic Graphene Oxide

Wang

Liu

Ying

et al. 2018

IJERPH

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In the current study, graphene oxide, Fe3+, and Fe2+ were used for the synthesis of magnetic graphene oxide (MGO) by an in situ chemical coprecipitation method. Scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction were used to characterize the well-prepared MGO. The prepared MGO was used as an adsorbent to remove five typical estrogens (estrone (E1), 17β-estradiol (E2), 17α-ethinylestradiol (17α-E2), estriol (E3), and synthetic estrogen (EE2)) at the ppb level from spiked ultrapure water and wastewater treatment plant effluent. The results indicated that the MGO can efficiently remove estrogens from both spiked ultrapure water and wastewater treatment plant effluent in 30 min at wide pH ranges from 3 to 11. The temperature could significantly affect removal performance. A removal efficiency of more than 90% was obtained at 35 °C in just 5 min, but at least 60 min was needed to get the same removal efficiency at 5 °C. In addition, an average of almost 80% of the estrogens can still be removed after 5 cycles of MGO regeneration but less than 40% can be reached after 10 cycles. These results indicate that MGO has potential for practical applications to remove lower levels of estrogens from real water matrixes and merits further evaluation.

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17α-Ethynylestradiol biodegradation in different river-based groundwater recharge modes with reclaimed water and degradation-associated community structure of bacteria and archaea

Cited by 32 publications

References 34 publications

Identification of novel catabolic genes involved in 17β‐estradiol degradation by Novosphingobium sp. ES2‐1

Identification of novel catabolic genes involved in 17β‐estradiol degradation by Novosphingobium sp. ES2‐1

Degradation of Micropollutants by UV–Chlorine Treatment in Reclaimed Water: pH Effects, Formation of Disinfectant Byproducts, and Toxicity Assay

Adsorption of Trace Estrogens in Ultrapure and Wastewater Treatment Plant Effluent by Magnetic Graphene Oxide

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