17β-Estradiol enhances contact hypersensitivity and IFN-γ expression in inflamed skin of BALB/c mice

Sakazaki, Fumitoshi; Ueno, Hitoshi; Nakamuro, Katsuhiko

doi:10.1016/j.toxlet.2006.05.013

Cited by 10 publications

(17 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…26,27) In that study, E2 administration led to an increase in ear swelling and in the expression of IFN-γ mRNA, 6 hr after the elicitation of the allergy.…”

Section: Introductionmentioning

confidence: 87%

“…BALB/c mice (Japan SLC Co., Shizuoka, Japan) were treated as previously described. 10,[26][27][28] Briefly, all treatments and maintenance of animals were conducted in a specific pathogen-free room at 23 ± 1 • C and 47-67% humidity, under a 12 hr light-dark cycle (lights on at 7:00 a.m.). Three-week-old mice were gonadectomized under pentobarbital anesthesia, and 3.2 µg of E2 (Wako Pure Chemical Industries Ltd., Osaka, Japan) dissolved in 100 µl of olive oil was subcutaneously injected (0.32 mg/kg body weight).…”

Section: Methodsmentioning

confidence: 99%

“…[26][27][28] Expression of IFN-γ mRNA was observed in the inflamed auricles of vehicle-treated mice from 6 to 9 hr after the elicitation of allergy. There was significantly higher IFN-γ expression in E2-treated mice than in vehicle-treated mice, at 6 hr (Fig.…”

Section: E2 Enhances Expression Of Ifn-γ Mrnamentioning

confidence: 98%

See 2 more Smart Citations

17.BETA.-Estradiol Enhances Interleukin-18 mRNA Expression after Sensitization of Mice with Contact Hypersensitivity

Sakazaki

Fujiyama

Ueno

et al. 2009

JOURNAL OF HEALTH SCIENCE

Self Cite

View full text Add to dashboard Cite

To clarify the mechanism underlying the enhancing effect of 17β-estradiol (E2) on contact hypersensitivity (CHS) and the expression of interferon (IFN)-γ in mice, the mRNA expression levels of interleukin (IL)-18 were evaluated. Female BALB/c mice aged 3 weeks were ovariectomized, administered 3.2 µg of E2, and sensitized by 50 µl of 3% 4-ethoxymethylene-2-phenyl-2-oxazolin-one (OXA). Seven days later, CHS was elicited by the application of 7.5 µl of 1% OXA on the ear auricles. The auricles, cervical lymph nodes and spleens were excised, and gene expression was evaluated by reverse transcription-polymerase chain reaction. E2 enhanced the expression of IL-18 mRNA in the spleen on the following day and in the ear auricles on days 4 and 7 after sensitization with OXA. The preadministration of an antibody against IL-18 receptor suppressed the CHS and reduced IFN-γ mRNA expression in E2-administered mice. IL-18 was present in the dermis of the ear skin and absent in the epidermis. E2 also enhanced the expression of IFN-γ and IL-18 mRNAs in splenocytes cultured with lipopolysaccharide (LPS). IL-18 protein was detected by flow cytometry in CD4 + , CD8 + and NKG2 + lymphocytes among splenocytes cultured with LPS. These results suggest that E2 enhances lymphocyte activation in the sensitization phase of CHS, and that IFN-γ mRNA expression is enhanced in the elicitation phase of CHS.

show abstract

“…26,27) In that study, E2 administration led to an increase in ear swelling and in the expression of IFN-γ mRNA, 6 hr after the elicitation of the allergy.…”

Section: Introductionmentioning

confidence: 87%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

17.BETA.-Estradiol Enhances Interleukin-18 mRNA Expression after Sensitization of Mice with Contact Hypersensitivity

Sakazaki

Fujiyama

Ueno

et al. 2009

JOURNAL OF HEALTH SCIENCE

Self Cite

View full text Add to dashboard Cite

show abstract

“…BALB/c mice (Japan SLC Co., Shizuoka, Japan) were treated as previously described. [15][16][17] Briefly, all treatments and maintenance of animals were conducted in a specific pathogen-free room at 23 ± 1 • C and 47-67% humidity, under a 12-hr lightdark cycle (lights on at 7:00 AM). Three-weekold mice were gonadectomized under pentobarbital anesthesia, and parabens dissolved in 100 µl of olive oil was subcutaneously injected.…”

Section: Methodsmentioning

confidence: 99%

“…12) We previously reported that 17β-estradiol augments contact hypersensitivity induced by 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (OXA) in the mouse auricle, which was used as a model of contact dermatitis. [15][16][17] In that study, 17β-estradiol administration led to an increase in the expression of interleukin (IL)-18 mRNA on the seventh day after sensitization of mice, in the expression of interferon (IFN)-γ mRNA at 6 hr after allergy elicitation, and, finally, in ear swelling. However, unlike 17β-estradiol, parabens have not been examined for their enhancing effects on contact hypersensitivity.…”

Section: Introductionmentioning

confidence: 95%

<i>p</i>-Hydroxybenzoate Esters Enhance Mouse Contact Hypersensitivity

Sakazaki

Ueno

Nagase

et al. 2009

JOURNAL OF HEALTH SCIENCE

Self Cite

View full text Add to dashboard Cite

p-Hydroxybenzoate esters (parabens), which are often used in cosmetics as preservatives, have been reported to have estrogenic and anti-androgenic effects and to cause contact dermatitis. In this paper, we evaluate the effects of parabens on contact hypersensitivity, an allergic response to low molecular weight chemicals that causes contact dermatitis. Female BALB/c mice were administered 1200 mg/kg of butylparaben and sensitized by painting 3% 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (OXA) on their backs. Seven days later, the mice were challenged by painting 1% OXA on the ear and the ear thickness was measured. Ear auricles were excised and the RNA expressions of interleukin (IL)-18 and interferon (IFN)-γ were evaluated by reverse transcription-polymerase chain reaction (RT-PCR). Butylparaben enhanced ear swelling at 6 hr after the elicitation of allergy. Butylparaben at a dosage of 600 mg/kg was sufficient to aggravate contact hypersensitivity. Among the six parabens examined, butylparaben exerted the strongest allergy-enhancing activity. Butylparaben also enhanced the RNA expression of IL-18 before the challenge with OXA and the RNA expression of IFN-γ at 6 hr after the challenge. These results suggest that parabens could enhance IL-18 and IFN-γ expression and exacerbate mouse contact hypersensitivity to OXA.

show abstract

Estrogen receptor α activation aggravates imiquimod‐induced psoriasis‐like dermatitis in mice by enhancing dendritic cell interleukin‐23 secretion

Iwano

Iwashita

Takagi

et al. 2020

J of Applied Toxicology

View full text Add to dashboard Cite

Our recent study has reported that estrogen receptors (ERs) are involved in several types of allergy development. This study aims to investigate the possible relationship between ER activation and development of imiquimod‐induced psoriasis‐like dermatitis. A mouse model of imiquimod‐induced psoriasis‐like dermatitis was generated by 5 days of topical application of 5% of imiquimod cream on the back of the ear and the shaved back skin of male BALB/c mice. From the second day of applying 5% imiquimod cream, either ERα selective agonist (propylpyrazoletriol [PPT] 2.5 mg/kg) or ERβ selective agonist (diarylpropionitrile, DPN; 2.5 mg/kg) was administered orally for four consecutive days. Immediately after the final imiquimod cream application, scratching behavior was video monitored for 2 hours. The ear‐swelling response was determined by comparing ear thickness before and after the final application of imiquimod cream. Twenty‐four hours after the final imiquimod application, back skin tissue and auricular lymph nodes were isolated under isoflurane anesthesia. Oral administration of PPT significantly induced itch behavior and proinflammatory responses, including the levels of interleukin (IL)‐17 and IL‐22, whereas DPN treatment did not influence either pruritic or proinflammatory responses. In addition, IL‐23 contribution by dendritic cells was identified using ER agonists on pretreated lipopolysaccharide (LPS)‐stimulated murine bone marrow derived dendritic cells (BMDCs). PPT also significantly enhanced IL‐23 secretion by LPS‐stimulated BMDCs. Our findings indicate that the activation of ERα, but not ERβ, is directly associated with inflammatory and pruritic responses in a mouse model of the imiquimod‐induced psoriasis by enhancing the secretion of IL‐23 by dendritic cells.

show abstract

17β-Estradiol enhances contact hypersensitivity and IFN-γ expression in inflamed skin of BALB/c mice

Cited by 10 publications

References 27 publications

17.BETA.-Estradiol Enhances Interleukin-18 mRNA Expression after Sensitization of Mice with Contact Hypersensitivity

17.BETA.-Estradiol Enhances Interleukin-18 mRNA Expression after Sensitization of Mice with Contact Hypersensitivity

<i>p</i>-Hydroxybenzoate Esters Enhance Mouse Contact Hypersensitivity

Estrogen receptor α activation aggravates imiquimod‐induced psoriasis‐like dermatitis in mice by enhancing dendritic cell interleukin‐23 secretion

Contact Info

Product

Resources

About