17β‐Estradiol mediates TGFBR3/Smad2/3 signaling to attenuate the fibrosis of TGF‐β1‐induced bovine endometrial epithelial cells via GPER

Fang, Zihao; Wang, Qian; Duan, Hongwei; Sheng, Xihui; Qi, Xiaolong; Xing, Kai; Liu, Bingying; Chang, Di; Guo, Yong; Wang, Xiangguo; Xiao, Longfei

doi:10.1002/jcp.31153

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…The BEECs were kindly gifted from Prof Guo Yong's team at the Beijng university of Agriculture [19]. Cells were cultured in DMEM/F12 (11330032, Gibco, California, USA) supplemented with 10% fetal bovine serum (FBS, 10091148, Gibco, California, USA).…”

Section: 、 Cell Culture and Treatmentmentioning

confidence: 99%

Melatonin alleviates palmitic acid–induced mitochondrial dysfunction by decreasing oxidative stress and enhancing autophagy combined in bovine endometrial epithelial cells

Wang,

Zhu,

Zhao

et al. 2024

Preprint

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Background: Negative energy balance (NEB) typically occurs in dairy cows after delivery, with those in high yielding being more likely to experience significant NEB. Such metabolic imbalance could cause ketosis, which is often accompanied by a decline in reproductive performance, but the molecular mechanism has not been fully elucidated. During excessive NEB, the body fat is extensively broken down, resulting in abnormal accumulation of non-esterified fatty acids (NEFA), represented by palmitic acid (PA), within the uterus. Such an abnormal accumulation has the potential to damage bovine endometrial epithelial cells (BEECs), while the molecular mechanisms underlying its involvement in the PA-induced injury of BEECs is poorly understood. Melatonin (MT) is recognized for its regulatory role in maintaining the homeostasis of mitochondrial reactive oxygen species (mitoROS). However, less is known about whether MT could ameliorate the damage of PA on BEECs and its molecular mechanism. Results: Our results demonstrated that 0.2 mM PA stress increased the level of cellular and mitochondrial oxidative stress. Further, we observed mitochondrial dysfunction, including an abnormal incidence of mitochondrial structure and respiratory function, reduction in mitochondrial membrane potential and mitochondrial copy number, which in turn induced apoptosis. Notably, we also found an upregulation of autophagy protein (PINK, Parkin, LC3B et al), however, the P62 protein was also increased. As we expected, 100uM MT pre-treatment attenuated PA-induced mitochondrial ROS and restored mitochondrial respiratory function. Meanwhile, pre-treated MT reversed the upregulation of P62 induced by PA and activate the AMPK-mTOR-Beclin-1 pathway, contributing to increase of autophagy and decline apoptosis. Conclusions: These findings indicate that PA could induce mitochondrial dysfunction and also enhance autophagy in BEECs. In addition, MT is hypothesized to not only reduce mitochondrial oxidative stress but also facilitate the clearance of damaged mitochondria through the regulation of autophagy pathways, thereby safeguarding the mitochondrial pool and promoting cellular viability. Our study helps better understand the molecular mechanism involved in excessive NEB's impact on the fertility outcomes of high yielding dairy cows.

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Section: 、 Cell Culture and Treatmentmentioning

confidence: 99%

Melatonin alleviates palmitic acid–induced mitochondrial dysfunction by decreasing oxidative stress and enhancing autophagy combined in bovine endometrial epithelial cells

Wang,

Zhu,

Zhao

et al. 2024

Preprint

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Screen-Printed Nanohybrid Palladium-Based Electrodes for Fast and Simple Determination of Estradiol in Livestock

Lopes,

Silva,

Fernandes

et al. 2024

ACS Omega

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One of the main challenges in animal breeding systems is determining estradiol (E2) in livestock samples as simple and minimally invasive as possible, Thus, a nonenzymatic biosensor screen-printed electrode (SPE) was developed by modifying nanohybrid palladium nanoparticles (PdNPs), and carbon dots anchored on a nanosilica particle (PdNPs/C.dots/SiO 2 ), denominated SPE/PdNPs/C.dots/SiO 2 , and successfully tested for the direct detection of estradiol in livestock samples. PdNPs were directly obtained by a one-step synthesis through carbon dot reduction. Hybrid nanomaterials were characterized by atomic force microscopy, high-resolution transmission electron microscopy, and electrochemical impedance. The combination of PdNPs with C.dots resulted in a nonenzymatic biosensor supported on a screen-printed platform with superior electrocatalytic properties regarding the oxidation of E2 when compared to unmodified sensors. Modifications in the working electrode resulted in high sensitivity toward E2 determination within a linear range from 0.005 to 14.0 μmol L −1 with a limit of detection of 1.0 nmol L −1 . The recovery rate of E2 in bovine serum samples and urine samples ranged from 92 to 106%. Interference studies showed that peak current variation (Δi p ) among all interferents evaluated and E2 did not exceed ±2%. The newly developed sensor stands out not only for its high sensitivity but also for its quick and simple way of production while also being disposable after analysis, providing a simple, sensitive, and practical approach for the determination of reproductive hormones in livestock.

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Melatonin alleviates palmitic acid-induced mitochondrial dysfunction by reducing oxidative stress and enhancing autophagy in bovine endometrial epithelial cells

Wang,

Gong,

Heng

et al. 2024

J Animal Sci Biotechnol

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Background Negative energy balance (NEB) typically occurs in dairy cows after delivery. Cows with a high yield are more likely to experience significant NEB. This type of metabolic imbalance could cause ketosis, which is often accompanied by a decline in reproductive performance. However, the molecular mechanisms underlying NEB have yet to be fully elucidated. During excessive NEB, the body fat is extensively broken down, resulting in the abnormal accumulation of non-esterified fatty acids (NEFAs), represented by palmitic acid (PA), within the uterus. Such an abnormal accumulation has the potential to damage bovine endometrial epithelial cells (BEECs), while the molecular mechanisms underlying its involvement in the PA-induced injury of BEECs remains poorly understood. Melatonin (MT) is recognized for its regulatory role in maintaining the homeostasis of mitochondrial reactive oxygen species (mitoROS). However, little is known as to whether MT could ameliorate the damage incurred by BEECs in response to PA and the molecular mechanism involved. Results Analysis showed that 0.2 mmol/L PA stress increased the level of cellular and mitochondrial oxidative stress, as indicated by increased reactive oxygen species (ROS) level. In addition, we observed mitochondrial dysfunction, including abnormal mitochondrial structure and respiratory function, along with a reduction in mitochondrial membrane potential and mitochondrial copy number, and the induction of apoptosis. Notably, we also observed the upregulation of autophagy proteins (PINK, Parkin, LC3B and Ubiquitin), however, the P62 protein was also increased. As we expected, 100 μmol/L of MT pre-treatment attenuated PA-induced mitochondrial ROS and restored mitochondrial respiratory function. Meanwhile, MT pretreatment reversed the upregulation of P62 induced by PA and activated the AMPK-mTOR-Beclin-1 pathway, contributing to an increase of autophagy and decline apoptosis. Conclusions Our findings indicate that PA can induce mitochondrial dysfunction and enhance autophagy in BEECs. In addition, MT is proved to not only reduce mitochondrial oxidative stress but also facilitate the clearance of damaged mitochondria by upregulating autophagy pathways, thereby safeguarding the mitochondrial pool and promoting cellular viability. Our study provides a better understanding of the molecular mechanisms underlying the effect of an excess of NEB on the fertility outcomes of high yielding dairy cows.

show abstract

17β‐Estradiol mediates TGFBR3/Smad2/3 signaling to attenuate the fibrosis of TGF‐β1‐induced bovine endometrial epithelial cells via GPER

Cited by 3 publications

References 41 publications

Melatonin alleviates palmitic acid–induced mitochondrial dysfunction by decreasing oxidative stress and enhancing autophagy combined in bovine endometrial epithelial cells

Melatonin alleviates palmitic acid–induced mitochondrial dysfunction by decreasing oxidative stress and enhancing autophagy combined in bovine endometrial epithelial cells

Screen-Printed Nanohybrid Palladium-Based Electrodes for Fast and Simple Determination of Estradiol in Livestock

Melatonin alleviates palmitic acid-induced mitochondrial dysfunction by reducing oxidative stress and enhancing autophagy in bovine endometrial epithelial cells

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