17β-Estradiol prevents oxidative stress and decreases blood pressure in ovariectomized rats

Hernández, Isabel; Delgado, Juan Luis; Díaz, José Carlos Losada; Quesada, T.; Teruel, María; Llanos, Ma Carmen; Carbonell, Luis F.

doi:10.1152/ajpregu.2000.279.5.r1599

Cited by 158 publications

(138 citation statements)

References 36 publications

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“…In our experimental model as well as in Hernández et al (17) , castration triggered a significant increase in LPO (Figure 3). We suggest that castration may have reduced the thiol groups, consistent with the significant decrease in GPx activity in C + PPVL, favoring the damage, which was evidenced by the increased LPO in this group as compared to the others.…”

Section: Histologysupporting

confidence: 85%

“…Borekci et al (18) demonstrated the protection of estrogen in the gastric mucosa, as other authors have demonstrated the greatest protection in the cardiovascular system in women of reproductive age compared with men the same age, for their hormonal and antioxidant activity (3,12,13,17,27) . Intact or castrated female rats were subjected to PPVL (pre-hepatic model) and then analyzed regarding the influence of castration (absence of estrogen) in PH-related changes, mainly to PHG.…”

Section: Discussionmentioning

confidence: 95%

“…Hernández et al (17) demonstrated that castration caused a decrease in thiol groups (-SH) and total antioxidant capacity in plasma as well as an increase in plasma lipoperoxides, reflecting an imbalance in favor of oxidative processes.…”

Section: Histologymentioning

confidence: 99%

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Protection of estrogen in portal hypertension gastropathy: an experimental model

Morgan-Martins

Jacques

Hartmann

et al. 2011

Arq. Gastroenterol.

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-Context -Portal hypertension is a complication secondary to cirrhosis that is characterized by increased blood flow and/or vascular resistance in the portal system, causing the appearance of a hyperdynamic collateral circulation. Partial portal vein ligation is an experimental model used in rats to study the pathophysiological mechanisms involved in pre-hepatic portal hypertension. Estrogen E2 is an antioxidant molecule with various physiological actions. Objectives -To evaluate the antioxidant activity of endogenous estrogen in an experimental model of partial portal vein ligation by comparing intact with castrated rats. Methods -Twenty Wistar rats, weighing on average 250 g were used and divided into four groups: sham-operated (SO); intact (I) with partial portal vein ligation (I + PPVL), castrated (C) and castrated with partial ligation of the vein (C + PPVL). Day 1: castration or sham-operation; day 7, PPVL surgery; on day 15 post-PPVL, portal pressure in the mesenteric vein of rats was measured on polygraph Letica. Lipid peroxidation in the stomach was assessed using the technique of thiobarbituric acid reactive substances and activity of antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase. Statistical analysis was done with ANOVA -Student-NewmanKeuls (mean ± SE), and P<0.05 was considered as significant. Results -Portal pressure was significantly increased in C + PPVL as compared to the other groups. There was no significant difference in the group of intact rats. TBARS showed significant damage in C and C + PPVL in relation to others. Antioxidant enzymes were significantly increased in the castrated rats with subsequent PPVL as compared to the other groups. Conclusion -We suggest that estrogen E2 plays a protective role in intact compared with castrated rats because it presents hydrophenolic radicals in its molecule, thus acting as an antioxidant in this experimental model.

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Section: Histologysupporting

confidence: 85%

Section: Discussionmentioning

confidence: 95%

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Protection of estrogen in portal hypertension gastropathy: an experimental model

Morgan-Martins

Jacques

Hartmann

et al. 2011

Arq. Gastroenterol.

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“…After surgery, the animals received an injection of antibiotics (40 000 U/kg penicillin G procaine IM). 12 …”

Section: Ovariectomymentioning

confidence: 99%

Exercise Training Improves Baroreflex Sensitivity Associated With Oxidative Stress Reduction in Ovariectomized Rats

Irigoyen

Paulini²,

Flores³

et al. 2005

Hypertension

104

130

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Abstract-The protection from coronary events that young women have is sharply reduced at menopause. Oxidative stress and baroreflex sensitivity impairment of the circulation have been demonstrated to increase cardiovascular risk. On the other hand, exercise training has been indicated as a nonpharmacological treatment for many diseases. The aim of the present study was to test the hypothesis that exercise training can improve baroreflex sensitivity associated with reduction in oxidative stress in ovariectomized rats, an experimental model of menopause. Exercise training was performed on a treadmill for 8 weeks. Arterial pressure and baroreflex sensitivity, which were evaluated by tachycardic and bradycardic responses to changes in arterial pressure, were monitored. Oxidative stress was evaluated by chemiluminescence and superoxide dismutase and catalase antioxidant enzyme activities. Exercise training reduced resting mean arterial pressure (112Ϯ2 vs 122Ϯ3 mm Hg in the sedentary group) and heart rate (325Ϯ4 vs 356Ϯ12 bpm in the sedentary group) and also improved baroreflex sensitivity (tachycardic response, 63% and bradycardic response, 58%). Myocardium (25%) and gastrocnemius muscle (48%) chemiluminescence were reduced, and myocardial superoxide dismutase (44%) and gastrocnemius catalase (97%) activities were enhanced in trained rats in comparison with sedentary rats. Myocardium chemiluminescence was positively correlated with systolic arterial pressure (rϭ0.6) and inversely correlated with baroreflex sensitivity (tachycardic response, rϭϪ0.8 and bradycardic response, rϭϪ0.7). These results indicate that exercise training in ovariectomized rats improves resting hemodynamic status and reflex control of the circulation, probably associated with oxidative stress reduction, suggesting a homeostatic role for exercise training in reducing cardiovascular risk in postmenopausal women. Key Words: exercise Ⅲ baroreflex Ⅲ oxidative stress Ⅲ rat Ⅲ estrogen Ⅲ menopause M enopause has been associated with impairment of aerobic fitness, muscle strength, and bone mineral density, as well as an increase in body weight, type 2 diabetes, osteoporotic fractures, and cardiovascular disease (CVD). 1 Many CVD states are associated with baroreflex impairment, the most important short-term regulator of arterial blood pressure. Moreover, the baroreflex has been recognized as a marker of autonomic control and as a predictor of CV mortality. 2 Estrogen deprivation induces endothelial dysfunction and autonomic impairment and increases oxidative stress in fertile young women 3 and postmenopausal women, 4,5 thus increasing the CV risk. Oxidative stress has been implicated in the pathophysiology of a large number of diseases, and it plays a possible mechanistic role in baroreflex dysfunction, because antioxidant substances seem to improve baroreflex sensitivity (BRS) in different species. 6 -9 However, the role of oxidative stress on CV autonomic dysfunction during estrogen deprivation is not well understood.Since the Women's Health Initiative...

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“…Deneysel çalışmalar da OVX ile oluşturulan östrojen yoksunluğunun redoks durumunu değiştir-diğini ve bu durumda uygulanan östrojen tedavisinin oksidan stresi azalttığını düşündürmüştür (41). Ayrıca, östrojen molekülünün kimyasal yapısının da serbest radikal süpürücü olarak etki ettiği ve fenol halkasındaki A pozisyonu sayesinde oksidan hasarı engellediği öne sürülmektedir (42).…”

Section: Discussionunclassified

Role of Ovarian Hormones in Psychological Stress-induced Oxidative Organ Damage in Rats

Memi¹,

Yeğen²

2016

Clin Exp Health Sci

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Amaç: Hipotalamus-hipofiz-gonad aksının etkisiyle, stres yanıtının cinsiyetler arasında farklılıklar gösterdiği bilinmektedir. Bu çalışmada postmenopozal dönemde over hormonlarının yokluğunun, psikolojik stres yanıtı ve oksidatif hasar üzerindeki rolünün araştırılması amaçlandı. Yöntemler: Dişi Sprague-Dawley sıçanlar (250-300 g, n=56) kontrol, taklit cerrahi ve overektomi (OVX) olarak 3 gruba ayrıldı. Anestezi sonrası taklit cerrahi ve OVX işlemi uygulandı. Cerrahi işlemden 60 gün sonra psikolojik stres oluşturmak üzere sıçanlar elektrik şokunun uygulandığı özel bir böl-meye yerleştirildiler. Ardından 3 gün daha aynı bölmede, aynı süre ile şok uygulanmadan tutuldular. Stres uygulamalarının 10'ar dakika öncesinde sıçanlara intraperitoneal yolla glukokortikoid reseptör antagonisti RU-486 (10 mg/kg) veya oksitosin reseptör antagonisti atosiban (1 mg/kg) veya serum fizyolojik verildi. Dördüncü günde delikli levha testi uygulanmasını takiben sıçanlar dekapite edilerek, doku ve kan örnekleri alındı. Bulgular: Psikolojik stres, RU-486 verilen gruplarda kortizol düzeylerini anlamlı olarak arttırırken atosiban verilen gruplarda azalttı. Stres uygulaması ile serum IL-1β düzeyleri artarken, serum TNF-α düzeylerinde değişiklik gözlenmedi. Stres, özellikle overektomi uygulanmış sıçanlarda mide, kolon ve beyin dokularında oksidatif hasarı arttırırken (p<0,05-0,001), RU-486'nın bu hasarı daha fazla arttırdığı, atosibanın ise bu etkiyi kısmen geri döndür-düğü gözlendi. Stres uygulaması ile azalan doku antioksidan miktarını RU-486 tedavisi değiştirmedi, ama atosiban kısmen arttırdı. Sonuç: Çalışmanın bulguları, psikolojik stres durumunda over hormonları-nın yokluğunun kortizol düzeyinde ya da anksiyete düzeyinde değişikliğe neden olmadığı halde, sitokin düzeylerinde ve dokuların oksidatif hasar durumlarında değişikliklere yol açabildiğini ortaya koymuştur. Anahtar kelimeler: Hipotalamus-hipofiz-adrenal bez aksı, hipotalamus-hipofiz-gonad aksı, glukokortikoid reseptörü, atosiban, oksitosin Özgün Araştırma / Original Article AbstractObjective: Stress response varies with respect to gender via the hypothalamic-pituitary-gonadal axis. We aimed to investigate the effect of ovarian hormone deficiency on psychological stress response and oxidative damage. Methods: Female Sprague Dawley rats (250-300 g, n=56) were divided as control, sham, and ovariectomy (OVX) groups. Sham operation or surgical OVX were conducted under anesthesia. After 60 days, the rats were placed in a special chamber to induce psychological stress by electric shock and were kept in the same chamber for 30 min on the following 3 days. Glucocorticoid receptor antagonist RU-486 (10 mg/kg), oxytocin receptor antagonist atosiban (1 mg/kg), or saline was intraperitoneally administered 10 min before stress exposure. After the hole-board anxiety test, the rats were decapitated on the 4th day; tissue and blood samples were obtained. Results: Psychological stress increased cortisol levels in the RU-486-administered group, while cortisol levels were decreased in the atosiban...

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17β-Estradiol prevents oxidative stress and decreases blood pressure in ovariectomized rats

Cited by 158 publications

References 36 publications

Protection of estrogen in portal hypertension gastropathy: an experimental model

Protection of estrogen in portal hypertension gastropathy: an experimental model

Exercise Training Improves Baroreflex Sensitivity Associated With Oxidative Stress Reduction in Ovariectomized Rats

Role of Ovarian Hormones in Psychological Stress-induced Oxidative Organ Damage in Rats

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