18 Effect of cystic fibrosis (CF) serum on the kinetics of phagocytosis of pulmonary alveolar macrophages (PAM)

Abstract

ARGENTINA The m a j o r i t y o f t h e p a t i e n t s s u r g i c a l l y t r e a t e d f o r b i l a t e r a lcryptorchidism b e f o r e puberty, a r e s t e r i l e . It seems t h a t t h i s could be due more t o a primary t e s t i c u l a r damage t h a n t o t h e age when s u r g i c a l c o r r e c t i o n was performed. Ten boys with monorchid (hi), 1 0 u n i l a t e r a l (LCQ) and 31 b i l a t e r a l c r y p t o r c h i d (DCQ) t e s t e s were s t u d i e d t o e v a l u a t e t e s t i c u l a r f u n c t i o n b e f o r e adulthood i n an i n t e n t t o p r e d i c t f u t u r e f e r t i l i t y . LI; and FSH l e v e l s were measured by R I A before and a f t e r Gn-RII (25 ug) administrat i o n . No d i f f e r e n c e between hi, LCQ and BCQ was found a t prepub e r t a l age. P a t i e n t s with BCQ a t p u b e r t a l age presented t v o dif f e r e n t responses: Group I (n=4) Ill ( ; -3C. S) b a s a l (b) 2 R i maximal response (mr) 1 4 2 3 0 ; FSH ( b ) 86_+22, (mr) 1 6 2 4 9 . Group I1 (n=6) LH (b) 71519, (mr) 38949; FSH (b) 333f40, (mr) 9172184, being s i g n i f i c a n t l y d i f f e r e n t LH response (p 0.01) and b and mr.FSH ( p 0.0025 and p 0.01 r e s p e c t i v e l y ) . I n 4 p a t i e n t s from t h e s e two groups t e s t i c u l a r byopses were obtained d u r i n g o r c h i d g pexy and demonstrated complete spermatogenesis i n p a t i e n t s o f Group I and s e v e r e t e s t i c u l a r damage i n Group 11. I n conclusion, Gn-RII s t i m u l a t i o n i n puberty g i v e s i n t e r e s t i n g d a t a r e g a r d i n g f k p~~.~~f;e?rL-;t-~ga~r~"g~fs i n b i l a t e r a l cryptorchid p a t i e n t s t r e a -QUANTITATION OF GERhiINAL EPITHELILhi I N PREPUBERTAL H i 14 MAN CRYPTORCHIDISM. GOTTLIEB S i l v i a , CHEhBS H., VILAR O., BERGADA C. CEDIE, Hospital de Nifios. Buenos Aires. ARGENTINA. There i s no u n i v e r s a l agreement about t h e t i m e o f treatment of cryptorchidism i n o r d e r t o p r e w n t s t e r i l i t y a f t e r puberty. Teg t i c u l a r biopsies were embedded i n Epon-Araldita and s e c t i o n o f 1~ were examined. S e r t o l i and germ c e l l s were i d e n t i f i e d . 17 t e s t i c u l a r biopsies from c r y p t o r c h i d t e s t e s (CQ) and 5 c o n t r a l a t e r a 1 s c r o t a 1 t e s t e s (EX) i n p a t i e n t s from 1 t o 10 y e a r s were s t u d i e d . S e r t o l i (s) and germ c e l l (G) q u a n t i t a t i o n was c a r r i e d o u t . The values obtained were ( ; %FN) S: 1535i82 (CQ) and 1835 ( SC) and G: 104215 (CQ) and 213-' 79 (SC). Evaluation o f t h e r e l a t i o n s h i p between number of germ c e l l s p e r l ( W S e r t o 1 i c e l l sr e v e a l s 2 d i f f e r e n t groups: Group A (n=12): 49% and Group B (n-5): 128219. In conclusion: 1 ) t h e number o f S e r t o l i c e l l s is p r a c t i c a l l y c o n s t a n t , independently o f t h e t e s t i c u l a r p o s i t i o n and t r e a t m e n t , 2 ) t h e l a c k o f development o f t h e germinal e p i t k l i u m o f t h e c r ...