18S rRNA Approach for Identification of Chara L. Species

Abstract

The study focused on the molecular phylogenetics for the identification of genus Chara species through 18S rRNA genes. Genus Chara sample is collected in the freshwater streams of Talakona region, Chittoor district, Andhra Pradesh and DNA was isolated. The 18S rRNA, gene sequence was used to carry out BLAST with the ‘nr’ database of NCBI GenBank database. First ten sequences were selected based on maximum identity score using multiple alignment software program Clustal W. Ribosomal Database Project was used for generating distance matrix and Molecular Evolutionary Genetics Analysis 6 Software was used for constructing the phylogenetic tree. Based on the maximum identifying score Chara species was identified as Chara foetida.