193 Feasibility of an Emit® Assay for Mycophenolic Acid in Plasma

Sensitive high-performance liquid chromatography–tandem mass spectrometry method for quantitative analysis of mycophenolic acid and its glucuronide metabolites in human plasma and urine

“…Amongst several quantification techniques that have been used to measure MPA in plasma, the EMIT immunoassay [20] is simple and requires minimal sample pre-treatment. However, its main weaknesses are that it does not measure MPA glucuronide metabolites and it is less specific than other methods.…”

Mycophenolate Mofetil Dose Reduction for Gastrointestinal Intolerance is Associated With Increased Rates of Rejection in Heart Transplant Patients

“…13 Furthermore, toxicity, as well as efficacy, is correlated to MPA levels, where patient tolerability declines as levels escalate. 17,18 These realities suggest a role for routine MPA testing in heart transplant patients to minimize both rejection and drug toxicity.…”

Identification of glucoside and carboxyl‐linked glucuronide conjugates of mycophenolic acid in plasma of transplant recipients treated with mycophenolate mofetil

“…After applying the concentrated fraction, the column was flushed with 2×4 ml H 2 O and the metabolite was then eluted with 2×0.5 ml acetonitrile/H 2 O (80/20 v/v). After evaporation of acetonitrile the eluate was subjected to analysis with the EMIT immunoassay for MPA ( Haley et al ., 1995 ) to test for cross‐reactivity and the rest was evaporated to dryness for mass spectrometry (MS) analysis. For MS analysis the sample was reconstituted using methanol/water (1/1 v/v).…”