196 Caffeine Consumption and the Colonic Mucosa-Associated Gut Microbiota

Abstract

INTRODUCTION: Studies have demonstrated health benefits of caffeine consumption, including decrease in cardiovascular disease, diabetes, and liver diseases. The exact mechanisms are not known. Caffeine consumption may possibly modulate the gut microbiome and therefore affect health and disease risk. We examined the association between caffeine consumption and the composition and structure of the colonic-gut microbiota. METHODS: In this study, 34 participants underwent a screening colonoscopy and had endoscopically normal colons. We obtained a total of 97 snap-frozen colonic mucosa biopsies from various segments of colon from these individuals. Microbial DNA was extracted, and subsequently amplified for the 16S rRNA gene V4 region and sequenced using the Illumina MiSeq platform. We analyzed the sequencing data using the UPARSE and SILVA database for operational taxonomic unit (OTU) classification. Self-administered BLOCK Food Frequency Questionnaire was used to ascertain daily caffeine consumption. We compared the diversity and relative abundance of bacterial taxonomies by high (≥82.9 mg) vs. low (<82.9 mg) consumption of caffeine. False discover rate (FDR) P-values were reported and <0.05 indicated statistical significance. RESULTS: The alpha diversity was the greatest in high caffeine consumers (Shannon index P < 0.0001). The beta diversity differed significantly between high vs. low caffeine drinkers (P = 0.0001). The composition of microbiomes did not differ at the phylum level based on caffeine consumption. At the genus level, high caffeine consumption was associated with increased relative abundance of Faecalibacterium (P < 0.0005) and Roseburia (P = 0.02), but decreased levels of Erysipelatoclostridium (P value <0.001) and an OTU belonging to the Lachnospiraceae family (Unc8895) (P < 0.0005). The observed association was seen regardless of age and dietary quality. Other bacteria commonly detected in gut microbiomes, including Odoribacter, Dialister, Fusicatenibactor, Alistipes, Blautia, and multiple members of Lachnospiraceae, were significantly more abundant (P < 0.05) in participants with higher caffeine consumption (Table 1). CONCLUSION: Higher caffeine consumption was associated with increased richness and evenness of the mucosa-associated gut microbiota, and higher relative abundance of anti-inflammatory bacteria, such as Faecalibacterium and Roseburia and lower levels of potentially harmful Erysipelatoclostridium.

Coffee Consumption Modulates Amoxicillin-Induced Dysbiosis in the Murine Gut Microbiome

“…Coffee is made up of several components that individually have the potential to change the gut microbiome, including caffeine, polyphenols, and fiber ( Gniechwitz et al, 2007 ; Liang and Kitts, 2015 ). Previous studies have investigated the effect of coffee on the microbiome, but to our knowledge there has not been extensive research investigating the combined effects of coffee and antibiotics on the gut microbiome ( Jaquet et al, 2009 ; Nakayama and Oishi, 2013 ; Gurwara et al, 2019 ). Food and drug interactions are important to study, as they have the potential to impact microbiome composition, function, and host health in ways that cannot necessarily be predicted ( Cabral D. et al, 2020 ; Cabral D. J. et al, 2019 ).…”

“…Thus, the differences observed between caffeinated and decaffeinated coffee may be the result of other components of coffee besides caffeine. In addition, physiological effects of coffee consumption such as decreased transit time of nutrients in the gut could be occurring in vivo leading to changes in microbial abundance ( Brown et al, 1990 ; Kashyap et al, 2013 ; Gurwara et al, 2019 ).…”

“…Related studies have shown that this effect of caffeine can lead to a decreased transit time of nutrients in the gut, which has been shown to change microbiome composition by affecting water and nutrient availability throughout the gut ( Brown et al, 1990 ; Kashyap et al, 2013 ). Caffeine has also been previously associated with a richer gut microbiome and may reduce the prevalence of inflammatory bacteria ( Gurwara et al, 2019 ).…”

Altered Gut Microbiota in Irritable Bowel Syndrome and Its Association with Food Components

“…In recent years, the interplay between diet and microbiota has emerged as an important pathological basis for IBS, which requires further investigation [ 4 , 13 , 14 ]. Moreover, the role of caffeine consumption on microbiome composition has been evaluated in different diseases, but limited studies have assessed the impact of caffeine in the IBS population [ 15 , 16 ]. Thus, in the present study, we aimed to assess the differences in nutrient intake and gut microbiota patterns between IBS and healthy control (HC) groups; meanwhile, we explored the associations between gut microbial community and food components in both IBS and HC groups.…”

Caffeine and Parkinson’s Disease: Multiple Benefits and Emerging Mechanisms

“…Caffeine is initially absorbed in the stomach and small intestine but is further fermented in the colon by gut microbiota (Scheperjans et al, 2015b). Recently, caffeine consumption is reportedly related to the colonic mucosaassociated gut microbiota (Gurwara et al, 2019); long-term coffee intake is associated with fecal microbial composition in humans, and regular consumption of coffee appears to be associated with changes in some intestinal microbiota groups in which caffeine, as the main dietary factor influencing PD development, may play a role (Gonzalez et al, 2020). Intestinal microorganisms also play a role in the metabolism of caffeine as caffeine was degraded in the gut of H. hampei, and that experimental inactivation of the gut microbiota eliminates this activity, suggesting that the detoxification of caffeine in H. hampei is mediated by the insect's gut microbiota (Ceja-Navarro et al, 2015).…”