19F NMR relaxation of buried tryptophan side chains suggest anisotropic rotational diffusion of the protein RfaH

Alam, Md Khushnood; Bhuvaneshwari, R. Aishwarya; Sengupta, Ishita

doi:10.1007/s10858-024-00450-x

J Biomol NMR

2024

DOI: 10.1007/s10858-024-00450-x

|View full text |Cite

19F NMR relaxation of buried tryptophan side chains suggest anisotropic rotational diffusion of the protein RfaH

Md Khushnood Alam,

R. Aishwarya Bhuvaneshwari,

Ishita Sengupta

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Insights into the Structure and Dynamics of Proteins from ¹⁹F Solution NMR Spectroscopy

Sengupta

2024

Biochemistry

View full text Add to dashboard Cite

19 F NMR spectroscopy has recently witnessed a resurgence as an attractive analytical tool for the study of the structure and dynamics of biomolecules in vitro and in cells, despite reports of its applications in biomolecular NMR since the 1970s. The high gyromagnetic ratio, large chemical shift dispersion, and complete absence of the spin 1/2 19 F nucleus from biomolecules results in background-free, highresolution 19 F NMR spectra. The introduction of 19 F probes in a few selected locations in biomolecules reduces spectral crowding despite its increased line width in comparison to typical 1 H NMR line widths and allows rapid site-specific measurements from simple 1D spectra alone. The design and synthesis of novel 19 F probes with reduced line widths and increased chemical shift sensitivity to the surrounding environment, together with advances in labeling techniques, NMR methodology, and hardware, have overcome several drawbacks of 19 F NMR spectroscopy. The increased interest and widespread use of 19 F NMR spectroscopy of biomolecules is gradually establishing it as a sensitive and high-resolution probe of biomolecular structure and dynamics, supplementing traditional 13 C/ 15 Nbased methods. This Review focuses on the advances in 19 F solution NMR spectroscopy of proteins in the past 5 years, with an emphasis on novel 19 F tags and labeling techniques, NMR experiments to probe protein structure and conformational dynamics in vitro, and in-cell NMR applications.

show abstract

Insights into the Structure and Dynamics of Proteins from ¹⁹F Solution NMR Spectroscopy

Sengupta

2024

Biochemistry

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

19F NMR relaxation of buried tryptophan side chains suggest anisotropic rotational diffusion of the protein RfaH

Cited by 1 publication

References 43 publications

Insights into the Structure and Dynamics of Proteins from ¹⁹F Solution NMR Spectroscopy

Insights into the Structure and Dynamics of Proteins from ¹⁹F Solution NMR Spectroscopy

Contact Info

Product

Resources

About

19F NMR relaxation of buried tryptophan side chains suggest anisotropic rotational diffusion of the protein RfaH

Cited by 1 publication

References 43 publications

Insights into the Structure and Dynamics of Proteins from 19F Solution NMR Spectroscopy

Insights into the Structure and Dynamics of Proteins from 19F Solution NMR Spectroscopy

Contact Info

Product

Resources

About

Insights into the Structure and Dynamics of Proteins from ¹⁹F Solution NMR Spectroscopy

Insights into the Structure and Dynamics of Proteins from ¹⁹F Solution NMR Spectroscopy