1H-31P home-built solid-state NMR probe with a scroll coil for 400-MHz NB magnet for biological lossy sample

Abstract

Phosphorus is one of the most important constituents of human biofilms, in particular lipid bilayer packing, phase transition (gel phase, physiological liquid crystal phase, ripple phase, non-biphasic), lipid head group orientation/ mechanics, and pure lipid bilayers. Phosphorus observations in biofilms play an important role in the study of the interaction of many types of proteins and biofilms in the human body. The design and construction of a 1 H-31 P double resonance probe with a scroll coil for solid-state NMR experiment are introduced. For good efficiency at the relatively high frequency, minimal RF sample heating during the experiment with a lossy sample, and a wide range of tuning frequency, this probe adapted the low inductance of a scroll coil. The 31 P NMR spectra obtained on the biological lossy sample were used to demonstrate the possibility for monitoring the protein dynamics on biomembrane mimetic system and phase change of phospholipid.

Structural and Mechanismic Studies of Lactophoricin Analog, Novel Antibacterial Peptide

“…The figure represents a 31 P 1D CP solid-state NMR spectrum of the bicelle with different concentrations of YK5. As shown in Figure 8 a, the bicelle lacking YK5 revealed 14-O-PC (−10.8 ppm) and DMPG (−7.2 ppm) in the 31 P spectrum [ 37 , 40 ], which represents the typical spectral pattern of a bicelle. Addition of 0.5 mM or 1 mM of YK5 to the bicelle ( Figure 8 b,c) maintained the peak pattern of 14-O-PC, but the peaks of DMPG and 6-O-PC were altered representing micelles or small vesicles [ 37 , 41 , 42 ].…”

“…Since the sample is highly viscous and sticky, it is difficult to use a commercial solid-state NMR probe. Therefore, a 1 H− 15 N solid-state NMR probe and 1 H− 31 P solid-state NMR probe were designed and constructed for experimental use [ 37 ].…”