1H HRMAS NMR spectroscopy and chemometrics for evaluation of metabolic changes in citrus sinensis Caused by Xanthomonas axonopodis pv. citri

Silva, Lorena; Filho, Elenilson G. Alves; Choze, Rafael; Lião, Luciano Morais; Alcantara, Glaucia Braz

doi:10.1590/s0103-50532012000600009

Cited by 26 publications

(8 citation statements)

References 19 publications

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“…Recent reports demonstrate that the laborious extraction procedures for metabolites has been improved, making metabolic analysis by NMR more practical (Fumagalli et al, 2009;Martineau et al, 2011;Rivas-Ubach et al, 2013). Additionally, high-resolution magic angle spinning (HR MAS) NMR provides the ability to use whole cell plant material to detect changes in abundant metabolites, which eliminates several preparation steps (Silva et al, 2012;Blondel et al, 2016).…”

Section: Nmr For Biomass Sugar and Fatty Acid Compositionmentioning

confidence: 99%

High Throughput Screening Technologies in Biomass Characterization

et al. 2018

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Biomass analysis is a slow and tedious process and not solely due to the long generation time for most plant species. Screening large numbers of plant variants for various geno-, pheno-, and chemo-types, whether naturally occurring or engineered in the lab, has multiple challenges. Plant cell walls are complex, heterogeneous networks that are difficult to deconstruct and analyze. Macroheterogeneity from tissue types, age, and environmental factors makes representative sampling a challenge and natural variability generates a significant range in data. Using high throughput (HTP) methodologies allows for large sample sets and replicates to be examined, narrowing in on more precise data for various analyses. This review provides a comprehensive survey of high throughput screening as applied to biomass characterization, from compositional analysis of cell walls by NIR, NMR, mass spectrometry, and wet chemistry to functional screening of changes in recalcitrance via HTP thermochemical pretreatment coupled to enzyme hydrolysis and microscale fermentation. The advancements and development of most high-throughput methods have been achieved through utilization of state-of-the art equipment and robotics, rapid detection methods, as well as reduction in sample size and preparation procedures. The computational analysis of the large amount of data generated using high throughput analytical techniques has recently become more sophisticated, faster and economically viable, enabling a more comprehensive understanding of biomass genomics, structure, composition, and properties. Therefore, methodology for analyzing large datasets generated by the various analytical techniques is also covered.

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Section: Nmr For Biomass Sugar and Fatty Acid Compositionmentioning

confidence: 99%

High Throughput Screening Technologies in Biomass Characterization

et al. 2018

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“…Citri, which is responsible for the citrus cancer. The authors revealed that the defensive metabolism activated by Citrus sinensis induced relevant changes in amino acids, carbohydrates, organic acids, and terpenoids content in the plant [74].…”

Section: Plant Response To Biotic and Abiotic Stressmentioning

confidence: 99%

HRMAS NMR spectroscopy applications in agriculture

Mazzei¹,

Piccolo²

2017

Chem. Biol. Technol. Agric.

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The relatively recent and advanced high-resolution magic angle spinning (HRMAS) NMR technique enables the direct application of NMR spectroscopy to semi-solid and gel-like samples. It combines the advantages of both solid-and liquid-state NMR by allowing to concomitantly measure intact and non-manipulated samples. Based on both 1D and 2D homo-and heteronuclear NMR spectra, HRMAS evaluates the composition of fresh semi-solid samples with a similar resolution as that of classical liquid-state NMR techniques. The enhanced spectral quality still obtained for semi-solid samples is mainly due to the MAS system, whose rapid spinning and sample orientation minimize the anisotropic processes that prevent the acquisition of meaningful NMR spectra for non-liquid materials. Moreover, HRMAS allows us to use edited pulse sequences which, especially in the case of biological tissues or agrofood products, may provide a simultaneous information on polar and non-polar components without the need of preliminary sample extraction. Additionally, this technique may differentiate molecular species according to their degree of mobility in hydrated matrices. The evident versatile potential of the HRMAS NMR makes this technique particularly useful for life science molecular studies. Despite the focus of HRMAS has been greatly devoted on clinical biomedicine, materials chemistry, and metabolomics, there are already enough studies that show useful applications on agricultural issues. This report reviews the latest representative studies that employ HRMAS NMR on systems related to agricultural chemistry, requiring the characterization and dynamics of soil components, plant tissues, agrofood products, and in vivo organisms.

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“…Neither are ideal for studying swollen rubber as drying and or extraction can both result in loss of information at interfaces and change important physical properties such as rigidity, and dynamics. In this regard, technological advances have emerged, such as high resolution magic angle spinning NMR (HR-MAS-NMR), that allow non-rigid solid materials (gels) and highly viscous and anisotropic liquids to be studied in a range of applications [17][18][19][20][21][22][23][24]. To probe interaction mechanisms and to study the phases separately, spectral editing methods are extremely useful.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive multiphase NMR spectroscopy: A new analytical method to study the effect of biodiesel blends on the structure of commercial rubbers

Silva

Filho

Simpson

et al. 2016

Fuel

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h i g h l i g h t sWe evaluate the effect of biodiesel on commercial rubber at molecular level. The new CMP-NMR probe was successful to study the phases in the fuel-rubber system. We observed that the biodiesel penetrates at EPDM despite the opposite report. It is observed the increasing on rubbers softening after biodiesel immersion. a b s t r a c t Biodiesel has been incorporated into the energy matrix in several countries, but its compatibility with some materials used in automotive engines is of growing concern. In the present study, comprehensive multiphase (CMP) NMR is applied to understand the different phases in fuel-rubber systems. CMP NMR is a novel technology that integrates all the hardware from solution-, gel-and solid-state into a single NMR probe, permitting all phases to be studied in samples in their natural unaltered state. Transverse relaxation experiments in combination with inverse diffusion editing permit the increasing mobility of the rubbers chains with biodiesel exposure to be monitored. Conversely diffusion editing and RADE experiments highlight the more rigid domains. In summary NR and NBR showed the absence of highly rigid domains after exposure to biodiesel whereas SBR and EPDM especially better retained their structural integrity. 13 C editing protocols and 13 C-1 H HSQC confirmed the increasing gel-like properties of NR, NBR and SBR with exposure to biodiesel. However, variable contact time experiments showed that biodiesel penetrates even the most resilient EPDM pores causing relaxation of the polymer chains and demonstrates that NMR is sensitive enough to highlight even the very slight swelling of the EPDM. Through these results it is possible to observe that the elastomer that exhibits the lowest susceptibility to biodiesel in molecular terms was EPDM, followed by SBR, NR, and NBR. It was also observed that biodiesel molecules were present in the EPDM structure despite the literature reporting its general resilience to biodiesel.

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1H HRMAS NMR spectroscopy and chemometrics for evaluation of metabolic changes in citrus sinensis Caused by Xanthomonas axonopodis pv. citri

Cited by 26 publications

References 19 publications

High Throughput Screening Technologies in Biomass Characterization

High Throughput Screening Technologies in Biomass Characterization

HRMAS NMR spectroscopy applications in agriculture

Comprehensive multiphase NMR spectroscopy: A new analytical method to study the effect of biodiesel blends on the structure of commercial rubbers

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