1H NMR-based Investigation of Metabolic Response to Electro-Acupuncture Stimulation

Lin, Caigui; Wei, Zhiliang; Cheng, Kian Kai; Xu, Jingjing; Shen, Guiping; She, Chang; Zhong, Huan; Chang, Xiao-Rong; Dong, Jiyang

doi:10.1038/s41598-017-07306-5

Cited by 20 publications

(11 citation statements)

References 50 publications

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“…Taken together, the EAGM and MBGM group showed metabolic changes in purine metabolism, glycine, serine and threonine metabolism, glycerophospholipid metabolism, valine, leucine and isoleucine degradation, pyruvate metabolism, TCA cycle, urea cycle, glycolysis, and glutamate metabolism. Previously, we had shown that meridian specificity is detectable at metabolome level [42]. Here, our current data also highlighted three pathways that were differentially perturbed due to stimulations at different meridian acupoints (Figures 5 and 9).…”

Section: Resultssupporting

confidence: 75%

“…Typical 1 H NMR spectra of extracted cortex, stomach, and liver tissues are shown in Figure S2. Previously, we had shown that electroacupuncture simulations on different meridians exerted distinctive metabolic impact (i.e., meridian specificity) [42]. Here, the NMR data also showed significant effects on metabolic profiles perturbed by electroacupuncture or moxibustion stimulations on SM and GM acupoints, which are discussed in Sections 3.4 and 3.5, respectively.…”

Section: Resultssupporting

confidence: 59%

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Metabolic Response in Rats following Electroacupuncture or Moxibustion Stimulation

Lin

Cheng

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Electroacupuncture and moxibustion are traditional Chinese medicine practices that exert therapeutic effects through stimulation of specific meridian acupoints. However, the biological basis of the therapies has been difficult to establish; thus the current practices still rely on ancient TCM references. Here, we used a rat model to study perturbations in cortex, liver, and stomach metabolome and plasma hormones following electroacupuncture or moxibustion treatment on either stomach meridian or gallbladder meridian acupoints. All treatment groups, regardless of meridian and mode of treatment, showed perturbation in cortex metabolome and increased phenylalanine, tyrosine, and branched-chain amino acids in liver. In addition, electroacupuncture was found to increase ATP in cortex, creatine, and dimethylglycine in stomach and GABA in liver. On the other hand, moxibustion increased plasma enkephalin concentration, as well as betaine and fumarate concentrations in stomach. Furthermore, we had observed meridian-specific changes including increased N-acetyl-aspartate in liver and 3-hydroxybutyrate in stomach for gallbladder meridian stimulation and increased noradrenaline concentration in blood plasma following stimulation on stomach meridian. In summary, the current findings may provide insight into the metabolic basis of electroacupuncture and moxibustion, which may contribute towards new application of acupoint stimulation.

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Section: Resultssupporting

confidence: 75%

Section: Resultssupporting

confidence: 59%

Metabolic Response in Rats following Electroacupuncture or Moxibustion Stimulation

Lin

Cheng

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…For analyzing the differential metabolites, the four-dimensional enhanced volcano plots were used for data visualization ( Lin et al, 2017 ). Based on OPLS-DA models, the VIP value and correlation coefficients (r) were calculated.…”

Section: Resultsmentioning

confidence: 99%

NMR-Based Metabolomics Analysis Predicts Response to Neoadjuvant Chemotherapy for Triple-Negative Breast Cancer

Zou

et al. 2021

Front. Mol. Biosci.

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Triple-negative breast cancer (TNBC) is the most fatal type of breast cancer (BC). Due to the lack of relevant targeted drug therapy, in addition to surgery, chemotherapy is still the most common treatment option for TNBC. TNBC is heterogeneous, and different patients have an unusual sensitivity to chemotherapy. Only part of the patients will benefit from chemotherapy, so neoadjuvant chemotherapy (NAC) is controversial in the treatment of TNBC. Here, we performed an NMR spectroscopy–based metabolomics study to analyze the relationship between the patients’ metabolic phenotypes and chemotherapy sensitivity in the serum samples. Metabolic phenotypes from patients with pathological partial response, pathological complete response, and pathological stable disease (pPR, pCR, and pSD) could be distinguished. Furthermore, we conducted metabolic pathway analysis based on identified significant metabolites and revealed significantly disturbed metabolic pathways closely associated with three groups of TNBC patients. We evaluated the discriminative ability of metabolites related to significantly disturbed metabolic pathways by using the multi-receiver–operating characteristic (ROC) curve analysis. Three significantly disturbed metabolic pathways of glycine, serine, and threonine metabolism, valine, leucine, and isoleucine biosynthesis, and alanine, aspartate, and glutamate metabolism could be used as potential predictive models to distinguish three types of TNBC patients. These results indicate that a metabolic phenotype could be used to predict whether a patient is suitable for NAC. Metabolomics research could provide data in support of metabolic phenotypes for personalized treatment of TNBC.

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“…Nuclear magnetic resonance (NMR)-based metabolomics, which has extensively applied to exploring therapeutic mechanisms of TCM including electro-acupuncture, can analyze quantities of samples during an extremely short time with the assistance of an auto sample instrument, moreover, can identify metabolic reactions and potential biomarkers [ 10 ]. Researchers found that the mechanism of electro-acupuncture in the treatment of GML may be related to brain-stomach interaction [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

NMR-based metabonomics reveals the dynamic effect of electro-acupuncture on central nervous system in gastric mucosal lesions (GML) rats

Huang

Peng

et al. 2022

Chin Med

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Background Gastric mucosal lesions (GML) are common in gastric diseases and seriously affect the quality of life. There are inevitable side effects in drug therapy. Acupuncture is an important part of traditional Chinese medicine. Electro-acupuncture (EA) has unique curative effect in treatment of GML. However, there are still few reports on the central mechanism of electro-acupuncture in treatment of GML. In this study, NMR metabonomics was used to explore the central metabolic change mechanism of electro-acupuncture in treatment of GML. Methods SD rats were randomly divided into Control, GML and EA groups. According to different intervention time, each group was further divided into 3 subgroups. This study mainly established GML model rats by 75% ethanol. Dynamic expressions of metabolites in cerebral cortex and medulla were observed by 1D 1H Nuclear Magnetic Resonance (NMR) metabolomics, combined with gastric mucosal histopathological examination to evaluate the time-effect relationship of electro-acupuncture at Zusanli (ST36) and Liangmen (ST21) points for 1 day, 4 days and 7 days treatment of GML. Results The results showed that the repair effect of electro-acupuncture on gastric mucosal injury was the most obvious in 4 days and stable in 7 days, and 4 days electro-acupuncture can effectively inhibit GML gastric mucosal inflammation and the expression of inflammatory cells. Meanwhile, the NMR spectrum results of medulla and cerebral cortex showed that, 21 potential metabolites were identified to participate in the mechanism of pathogenesis of GML and the regulation of electro-acupuncture, including 15 in medulla and 10 in cerebral cortex. Metabolic pathway analysis showed that the differential metabolites involved 19 metabolic pathways, which could be divided into energy, neurotransmitters, cells and cell membrane and antioxidation according to their functions. The correlation analysis of stomach, medulla and cerebral cortex shows that the stimulation signal of GML may reach the cerebral cortex from the stomach through medulla, and electro-acupuncture can treat GML by regulating the central nervous system (CNS). Conclusions 4 days electro-acupuncture treatment can significantly improve gastric mucosal injury, and the curative effect tends to be stable in 7 days treatment. Meanwhile, the pathogenesis of GML and the efficacy of electro-acupuncture involve metabolic pathways such as energy, neurotransmitters, cells and antioxidation, and electro-acupuncture can treat GML by regulating CNS.

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1H NMR-based Investigation of Metabolic Response to Electro-Acupuncture Stimulation

Cited by 20 publications

References 50 publications

Metabolic Response in Rats following Electroacupuncture or Moxibustion Stimulation

Metabolic Response in Rats following Electroacupuncture or Moxibustion Stimulation

NMR-Based Metabolomics Analysis Predicts Response to Neoadjuvant Chemotherapy for Triple-Negative Breast Cancer

NMR-based metabonomics reveals the dynamic effect of electro-acupuncture on central nervous system in gastric mucosal lesions (GML) rats

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