1H NMR-based metabolomics and UHPLC-ESI-MS/MS for the investigation of bioactive compounds from Lupinus albus fractions

Hellal, Khaoula; Mediani, Ahmed; Ismail, Intan Safinar; Tan, Chin Ping; Abas, Faridah

doi:10.1016/j.foodres.2020.110046

Cited by 7 publications

(2 citation statements)

References 64 publications

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“…A study based on the characterization of the metabolites in the active fraction of L. albus L. seeds was developed using ultra-high-performance LC–tandem mass spectrometry (UHPLC-MS/MS) analysis, performed by a hybrid quadrupole-orbitrap mass spectrometer [ 24 ]. LAs were also investigated in tissue sections of seed-containing pods of Lupinus angustifolius L. ( L. angustifolius L.), using matrix-assisted laser desorption ionization imaging (MALDI-MSI) [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Development of an HPLC-MS/MS Method for the Determination of Alkaloids in Lupins

et al. 2023

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Lupin alkaloids (LAs) represent a class of toxic secondary metabolites in plants, in particular in Lupinus spp.; they are produced as a defense mechanism due to their strong bitter taste and are very dangerous for human and animals. In this work, a sensitive and reliable high performance liquid chromatography—tandem mass spectrometry (HPLC-MS/MS) analytical method for the identification and quantification of thirteen lupin alkaloids was developed and validated according to FDA guidelines. Efficient extraction and clean-up steps, carried out by solid-phase extraction, were finely tuned on the basis of the characteristics of the analytes and lupin samples, providing good selectivity with minimized matrix interference. The effectiveness of the method was proven by the satisfactory recovery values obtained for most of the analytes and a matrix effect ≤23% for all tested levels. In addition, a sensitive and reliable determination of the target compounds was obtained; LOQs were between 1 and 25 µg Kg−1, i.e., below the requested maximum levels (<200 mg Kg−1). The method was applied to evaluate the LAs profile in different batches of raw L. albus L. samples, varying in size and across farming treatments.

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Section: Introductionmentioning

confidence: 99%

Development of an HPLC-MS/MS Method for the Determination of Alkaloids in Lupins

et al. 2023

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“…Some secondary metabolites are reported to help the plant to communicate with other organisms whilst some protect plants from abiotic stress e.g., UV-B radiation [103]. 1 H NMR based metabolomics study conducted on Lupinus albus or white lupine by Hellal et al [104] identified caprate, adenosine, and asparagine metabolites in Lupinus albus and were reported to be key compounds that might be responsible for the strong α-glucosidase inhibitory and DPPH radical scavenging activities. Prusinski [105] indicated that previous studies have reported that amino acids have a beneficial effect on the physiological condition of the human organism, especially for those suffering from diabetes.…”

Section: Discussionmentioning

confidence: 99%

1H NMR-Based Metabolomics Profile of Green and Red Amaranthus Grown in Open Field versus Greenhouse Cultivation System

Nemadodzi,

Managa

2023

Metabolites

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Traditionally, indigenous African leafy vegetables such as Amaranthus, blackjack, jute mallow, cleome monophyla, and spider plants have been conventionally and organically grown as weeds in open fields. However, the lack of land space due to the increase in population has resulted in unconventional, modern, and advanced agricultural farming. The introduction of a greenhouse has recently become the second most popular growing system alongside shade net and glasshouse to increase productivity and meet consumers’ demand. Several studies on Amaranthus species have solely focused on physiological parameters and nutritional composition, leaving a huge gap on their metabolomic profile of the leaves which is crucial to comprehend when growing Amaranthus species in different cropping systems. Therefore, the study aimed to determine the influence of different cropping systems on the release of metabolites of two commonly consumed Amaranthus species in South Africa. H1 -Nuclear Magnetic Resonance (NMR) tool was used to profile the untargeted metabolites of green (Amaranthus graecizans L.) and red (Amaranthus cruentus L.) species. A total of 12 metabolites—trehalose, betaine, glutamine, choline, sucrose, caprate, adenosine, asparagine, carnitine, caffeine, aspartate, and alanine—were detected in green amaranth grown in open fields. Except for caffeine, aspartate, and caprate, which were found in the green amaranth grown in open fields, all the other metabolites were detected in the greenhouse grown once. Interestingly, allantoin, which serves as an allelochemical, was the sole distinct metabolite detected in greenhouse cultivated green amaranth. On the contrary, seven similar metabolites were quantified in red amaranth grown in both open fields and greenhouses, apart from caffeine, which was only detected in greenhouse-cultivated red amaranth.

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Applications of some advanced sequencing, analytical, and computational approaches in medicinal plant research: a review

Singh,

Mittal,

Verma

et al. 2023

Mol Biol Rep

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1H NMR-based metabolomics and UHPLC-ESI-MS/MS for the investigation of bioactive compounds from Lupinus albus fractions

Cited by 7 publications

References 64 publications

Development of an HPLC-MS/MS Method for the Determination of Alkaloids in Lupins

Development of an HPLC-MS/MS Method for the Determination of Alkaloids in Lupins

1H NMR-Based Metabolomics Profile of Green and Red Amaranthus Grown in Open Field versus Greenhouse Cultivation System

Applications of some advanced sequencing, analytical, and computational approaches in medicinal plant research: a review

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