1H NMR-based urinary metabolic analysis of high-dose cyclophosphamide-induced toxicity in mice

Windows Scanning Multiomics: Integrated Metabolomics and Proteomics

“…Additionally, the contents of alanine and relevant proteins, Aars2 and Aldh5a1, decreased, while the contents of isoleucine and tryptophan increased in the CTX-treated group, indicating a perturbation in amino acid metabolism as another factor in hepatic damage. Similar findings were reported by Luo et al, who noted significant changes in energy metabolism and amino acid metabolism in mice injected with CTX . However, some of the relationships suggested by the conventional analysis seemed to be unrelated to hepatic damage.…”

“…Similar findings were reported by Luo et al, who noted significant changes in energy metabolism and amino acid metabolism in mice injected with CTX. 36 However, some of the relationships suggested by the conventional analysis seemed to be unrelated to hepatic damage. These included associations like the one between ascorbate and aldarate metabolism, linking ascorbic acid and proteins Ugt2b1, Akr1a1, and the connection between butanoate metabolism and fumaric acid with the Aldh5a1 protein.…”

Activation of CYP3A by Accelerated Blood Clearance Phenomenon Potentiates the Hepatocellular Carcinoma-Targeting Therapeutic Effects of PEGylated Anticancer Prodrug Liposomes

“…Although CP is clinically known for its high organ toxicity, this method of administration did not cause significant toxicity to other organs based on H&E staining of the other major organs in mice (Luo et al, 2023), probably because the 5 mg/mL dose given once every 6 days did not reach the damaging dose (Supplemental Fig. S4).…”