1H-NMR metabolomics-based classification of Japanese sake and comparative metabolome analysis by gas chromatography–mass spectrometry

Multifunctional-separation-mode ion chromatography method for determining major metabolites during multiple parallel fermentation of rice wine

“…Validation tests for ethanol, glucose, organic acids, and oligosaccharides were performed using 11 brewing-processed rice wine samples (collected on days 7, 9-13, and [15][16][17][18][19]. For glucose, the concentrations obtained using the developed method and a spectrophotometric method with an enzyme-based 4-aminoantipyrine colorimetric reagent were compared.…”

“…Nevertheless, such advanced instrumentation is expensive and difficult to introduce into small-and medium-sized companies. 1,[13][14][15][16] Japanese rice wine breweries are a representative example of small-and medium-sized companies for which metabolite analysis could be useful. During rice wine brewing, the process of transforming rice into ethanol involves saccharication, glycolysis, the tricarboxylic acid (TCA) cycle, and ethanol fermentation.…”

“…Nevertheless, such advanced instrumentation is expensive and difficult to introduce into small- and medium-sized companies. 1,13–16…”

Advances and Trends in Omics Technology Development

“…Methods for metabolome interrogation include Fourier transform-infrared (FT-IR) spectroscopy ( 205 ), Raman spectroscopy ( 206 ), NMR spectroscopy ( 18 , 207 ), MS-based approaches such as MS ( 20 ), MS/MS ( 208 ), liquid chromatography (LC)-MS ( 209 ), gas chromatography (GC)-MS ( 210 , 211 ), as well as others.…”

“…MS is the most feasible tool to probe the metabolome that can detect a wide spectrum of metabolites ( 216 ). Isobaric metabolites can be distinguished by various MS/MS ( 208 ) and/or being coupled with LC ( 209 ) or GC ( 210 , 211 ).…”