1H-NMR Relaxation Investigation of Water Bound to Bovine Rod Outer Segment Disk Membranes

Gaggelli, Elena; Niccolai, Neri; Valensin, Gianni

doi:10.1016/s0006-3495(82)84701-2

Cited by 5 publications

(6 citation statements)

References 14 publications

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“…Assuming the 1/ T 1,b values for copolymer gels to be similar, then the increases in bound water seem to be in agreement with the above written order, indicating that hydrophobic copolymer gels are less hydrated than hydrophilic copolymer gels . The obtained results were similar to the reported plots for frog muscle 27, biological membranes 28, 29, bovine mercaptalbumin (BMA) solutions, and BSA*gels 5.…”

Section: Discussionsupporting

confidence: 87%

“…To study whether there is a significant difference in 1/ T 1 values between the paired experiments or not, we carried out 1/ T 1 vs X/(1 – X) analyses 5, 13, 27–29. As shown in Figure 1c, the obtained regression lines for intact and cataractous lenses were 1/ T 1 = 0.39X/(1 – X) + 0.33 and 1/ T 1 = 0.48X/(1 – X) + 0.34 (linearity test; n = 14; p < 0.005, Student's t ‐test), respectively, exhibiting some indication of increases in the fraction of bound water during cataract formation, as increased hydration (water content) in the localized cataracts of excised intact rabbit lenses 33 and in hereditary cataract mouse lenses detected by laser Raman spectroscopy 34, 35.…”

Section: Discussionmentioning

confidence: 96%

“…The 1/ T 1 vs W (%) plots were re‐analyzed using 1/ T 1 vs X/(1 – X) plots, as shown in Figure 1c. It has been shown that cross‐relaxation between water protons and macromolecular protons provides the most effective relaxation pathway for the water molecules in the immediate vicinity of the macromolecular surface 26–29. If the two‐phase model of water molecules (free and bound) exchanging rapidly is assumed, the observed 1/ T 1 parameters can be successfully expressed as follows: 1/ T 1 vs X/(1 – X) plots, i.e.…”

Section: Discussionmentioning

confidence: 99%

“…If the two‐phase model of water molecules (free and bound) exchanging rapidly is assumed, the observed 1/ T 1 parameters can be successfully expressed as follows: 1/ T 1 vs X/(1 – X) plots, i.e. 1/ T 1 = C[1/ T 1,b ‐ 1/ T 1,f ]X/(1 – X) + 1/ T 1,f , where X is the weight fraction of macromolecules, T 1,f and T 1,b are the spin‐lattice relaxation times for free and bound water, respectively, and C is the amount of bound water (g/g macromolecule) 5, 13, 27–29. It can be seen from Figure 1c that plots for copolymer gels exhibited increases of slope in the following order: gels‐4 < gels‐3 < gels‐2 ≈ gels‐1.…”

Section: Discussionmentioning

confidence: 99%

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Phase Diagram of Electrostricted H₂O at Surfaces of Electrodes at 273–373 K: Electric Critical Point of Water

Danielewicz-Ferchmin

Ferchmin

2005

ChemPhysChem

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The equation of state of an open system containing water in an electric field of strength E greater than 108 V m-1 leads to a phase diagram with two-phase and single-phase regions in the Pi,E,T coordinates (=electrostriction pressure). The phases differ by their degree of orientation of H2O molecules in the field. The phase transition is discontinuous with respect to E. The calculation shows that the discontinuity vanishes at the critical point: Pi(c) approximately 0.2 GPa, Ec approximately 109 V m-1 and below 313 K. The thermal effect of the transition is calculated and compared with an experiment at the Hg/aqueous electrolyte interface found in the literature.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Phase Diagram of Electrostricted H₂O at Surfaces of Electrodes at 273–373 K: Electric Critical Point of Water

Danielewicz-Ferchmin

Ferchmin

2005

ChemPhysChem

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“…This condition has been shown to exist in protein solutions (Berendsen, 1975). Kuntz, 1974), 3) the conformation of the protein (Dodson,19S4), and 4) the concentration of lipids (Gaggelli, 1982). The presence of paramagnetic substances also affects relaxation parameters.…”

Section: Molecular Environmentmentioning

confidence: 99%

NMR imaging and spectroscopy of the mammalian central nervous system after heavy ion radiation

Richards¹

1984

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CHAPTER 1.-INTRODUCTION 1.1 Purpose and scope 1 1.2 Acknowledgements 1.3 Background 1.3.1 Radiation biophysics of the CNS 1.3.2 Relationship between NMR relaxation parameters and CNS pathology CHAPTER 2.-SPIN-ECHO IMAGING WITH PROJECTION RECONSTRUCTION 2.1 Methods 2.2 Results CHAPTER 3.-2D FOURIER TRANSFORM SPIN-ECHO IMAGING 3.1 Methods 3.2 Results CHAPTER 4.-SATURATION RECOVERY IMAGING WITH PROJECTION RECONSTRUCTION 4.1 Modification of the UCB-180 spectrometer 42 for imaging 4.2 Phantom studies 50 4.3 Methods 53 4.3.1 Helium beam irradiation of the CNS 53 4.3.2 The NMR experiment 4.3.3 Tl relaxation tine calculation 4.4 Results CHAPTER 5.-SPIN-ECHO SPECTROSCOPY WITH RF 64 ENERGY OPTIMIZATION 5.1 Localization in surface coil spectroscopy 65 5.2 Proton spectroscopy of irradiated rat brain 75 5.3 Results 76 CHAPTER 6.-2D FOURIER TRANSFORM PROTON SPECTROSCOPY 82 6.1 Introduction 82 6.2 Instrumentation and computer software 82 6.3 Method 85 6.3.1 Magnetic field gradient orientation 85 6.3.2 The NMR experiment 86 b.4 Results 87 CHAPTER 7.-PROTON CHEMICAL SHIFTS OF AQUEOUS AND ORGANIC 94 FRACTIONS OF BRAIN EXTRACTS 7.1 Methods 94 7.1.1 Brain preparation 94 7.1.2 The NMR experiment 7.1.3 Calibration of NMR peak areas with chemical concentration 7.1.4 Chemical shifts of isolated chemicals 7.2 Results 103 CHAPTER 8.-HISTOLOGY OF IRRADIATED BRAINS 112 8.1 Methods 112 8.2 Results 113 CHAPTER 9.-SUMMARY AND DISCUSSION APPENDIX Computer program listings BIBLIOGRAPHY This report was done with support from the Depai tment of Energy. Any conclusions or opinions expressed in this report represent solely those of the authors) and not necessarily those of The Regents of the University of California, the Lawrence Berkeley Laboratory or the Department of Energy. Reference to a company or product name does not imply approval or recommendation of the pioduct by the University of California or the U.S. Department of Energy to the exclusion of others that may be suitable. diffuse through the tissue and react with the biologically important macromolecules. This secondary phenomenon is known as the indirect effect of radiation and occurs between 10E-10 and 10E-3 seconds. The products formed from these interactions would be organic peroxides, organic free radicals, organic hydroperoxides, and hydrogen peroxide (Singh and Singh 1982). The consequences of interaction with these products include: 1) DNA and RNA damage (Okada, 1979); 2) enzyme inactivation and activation (Altman, 1970), .1) oxidation of sulfides; 4) lipid peroxidation (Pritchard and Singh, 1966); and conformational changes in protein structure (Todo, 1982). 1.3.2.4 Early biological damage The iianediate biological effect of radiation at the cellular level is an alteration in the biochemical processes directing and controlling RNA synthesis, and the synthesis of proteins and lipids necessary for structure and metabolism of the cell. These effects occur between 10 seconds and 10 hours (Singh and Singh 1982). Damage done to DNA is particularly important because the same base-pair ...

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Comparative ¹H NMR studies of saturation transfer in copolymer gels and mouse lenses

et al. 2010

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Saturation transfer in cross-linked copolymer gels and excised intact and perforating trauma-induced cataract mouse lenses (4- or 8-week-old) were studied using intermolecular cross-relaxation rates (1/T(IS)(H(2)O); 1/T(IS)), monitored with f(2)-irradiation at -8.79, -4.00, and 7.13 ppm (gammaH(2)/2pi approximately 69 Hz). [1] The 1/T(IS)(7.13 ppm) vs dry weight [W (%)] profiles for hydrophilic copolymer gels were far steeper than those for hydrophobic copolymer gels, indicating the participation of an amount of bound water and a number of copolymer hydroxyl groups in the saturation transfer process. In contrast, the 1/T(IS)(-8.79 ppm) vs W (%) profiles exhibited little difference between the hydrophilic and hydrophobic copolymer gels, indicating the major participation of molecular rigidity, i.e. W (%) in the saturation transfer process. [2] The 1/T(IS)(7.13 ppm) values for cataractous mouse lenses were larger than those for intact lenses, indicating the formation of large, immobile lens protein associates or aggregates containing a sufficient amount of bound water for the saturation transfer. [3] The 1/T(IS)(7.13 ppm) vs W (%) profiles for the hydrophilic copolymer gels exhibited similar characteristics to the intact and cataractous mouse lenses with regard to the saturation transfer process.

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1H-NMR Relaxation Investigation of Water Bound to Bovine Rod Outer Segment Disk Membranes

Cited by 5 publications

References 14 publications

Phase Diagram of Electrostricted H₂O at Surfaces of Electrodes at 273–373 K: Electric Critical Point of Water

Phase Diagram of Electrostricted H₂O at Surfaces of Electrodes at 273–373 K: Electric Critical Point of Water

NMR imaging and spectroscopy of the mammalian central nervous system after heavy ion radiation

Comparative ¹H NMR studies of saturation transfer in copolymer gels and mouse lenses

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1H-NMR Relaxation Investigation of Water Bound to Bovine Rod Outer Segment Disk Membranes

Cited by 5 publications

References 14 publications

Phase Diagram of Electrostricted H2O at Surfaces of Electrodes at 273–373 K: Electric Critical Point of Water

Phase Diagram of Electrostricted H2O at Surfaces of Electrodes at 273–373 K: Electric Critical Point of Water

NMR imaging and spectroscopy of the mammalian central nervous system after heavy ion radiation

Comparative 1H NMR studies of saturation transfer in copolymer gels and mouse lenses

Contact Info

Product

Resources

About

Phase Diagram of Electrostricted H₂O at Surfaces of Electrodes at 273–373 K: Electric Critical Point of Water

Phase Diagram of Electrostricted H₂O at Surfaces of Electrodes at 273–373 K: Electric Critical Point of Water

Comparative ¹H NMR studies of saturation transfer in copolymer gels and mouse lenses