1H-NMR study of the effect of acetonitrile on the interaction of ibuprofen with human serum albumin

A competitive low-affinity binding model for determining the mutual and specific sites of two ligands on protein

“…to that used for the low-affinity binding of a single ligand to protein [20][21][22][23][24][25]37,38].…”

“…And a similar mathematical expression has been tested using the displacement isothermal titration calorimetry method [19]. As for low-affinity binding, a fast and reversible chemical-reaction (FRC) model has been widely accepted and used to derive the number of binding sites and apparent dissociation constant (K d ) [20][21][22][23][24][25]. The competitive binding of two drugs on the low-affinity sites of human serum albumin (HSA) has also been investigated using NMR method [17].…”

Effect of metal ions on the interaction between bovine serum albumin and berberine chloride extracted from a traditional Chinese Herb coptis chinensis franch

“…It is well known that this equation can be applied not only to gas-solid interaction but also to liquid-solid interaction. Ji et al [30] have applied the equation to evaluate the dynamic parameters of the ligand-protein complex. In this paper, Langmuir adsorption isotherm is applied to obtain the apparent association constant K A , and binding sites n. The binding process can be described by the following equation:…”

Synthesis of a new gadolinium complex with a high affinity for human serum albumin and its manifold physicochemical characterization by proton relaxation rate analysis, NMR diffusometry and electrospray mass spectrometry

“…Its interaction with HSA was evaluated by three different techniques: proton relaxation rate analysis [7][8][9][10][11][12][13][14][15][16][17][18][19], NMR diffusometry [29][30][31][32][33][34][35][36][37][38][39], and electrospray mass spectrometry [19,40]. The first method takes advantage of the relaxivity difference between the free paramagnetic complex and the supramolecular assembly resulting of its binding to HSA.…”