1HNMR-based metabolomic profile of rats with experimental acute pancreatitis

Li, Juan; Zhao, Xiaojing; Liu, Yi-xia; Peng, Xiao-Hang; Zhu, Shifeng; Guo, Hui; Liu, Yi-Ling; Wan, Mei-Hua; Tang, Wen-Fu

doi:10.1186/1471-230x-14-115

Cited by 15 publications

(12 citation statements)

References 31 publications

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“…In Fig. 2C, according to some other studies on serum metabonomics,28, 32, 33 the unknown metabolites may be high-density lipoprotein which indicated that patients may have up-regulated lipid metabolism at the seizure stage. The energy consumption of patients at the seizure stage is huge, and the carbohydrate supply is insufficient.…”

Section: Discussionmentioning

confidence: 75%

1H NMR-based metabolomic study of metabolic profiling for pollinosis

Zhou

et al. 2019

World Allergy Organization Journal

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Background Allergic rhinitis is the main symptom of pollinosis, relieved by non-specific treatment universally. This study aimed to find the changes of serum metabolites between the seizure and remission periods of pollinosis and provide assistance in the diagnosis and/or therapy. Methods Metabonomics based on 1 H nuclear magnetic resonance (NMR) was used to study the 37 serum samples of pollinosis patients. Results We believed that the decreased levels of isoleutine, leutine, valine, 3-hydroxybutyric acid, allo-threonine, alanine, methionine, glutamine, lysine, glycine, l -tyrosine, histidine, phenylalanine, lactate, acetate, O-acetylcholine, creatine and creatinine and the increased level of N -acetylglutamine at the seizure stage were statistically significant. Conclusions Pollinosis could change the metabolic profiles of energy, amino acid and lipid in patients, which might be the diagnosis and/or prognosis markers for hay fever patients.

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Section: Discussionmentioning

confidence: 75%

1H NMR-based metabolomic study of metabolic profiling for pollinosis

Zhou

et al. 2019

World Allergy Organization Journal

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“…The reduced glucose metabolism leads to reduce pyruvate production, which eventually results in diminished mitochondrial energy metabolism and ATP levels [ 61 ]. Meanwhile, an increased lactate level indicated that anaerobic glycolysis has occurred and is not related to the main energy source pathway under normal conditions [ 12 , 14 ]. Moreover, the lactate level increased with an increased level of ketogenic amino acid such as leucine that acts as a gluconeogenic precursor [ 13 , 71 ].…”

Section: Discussionmentioning

confidence: 99%

“…It showed that anaerobic glycolysis might occur to balance the metabolism dysfunction due to glucose hypometabolism. An increased level of succinate seen in Abeta group, together with lactate level might also due to anaerobic glycolysis [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…Metabolomics is the latest approach to study biological samples and can produce detailed data on the metabolic changes that occur in an organism in specific pathophysiologic states [ 12 ]. It has been highly utilized as a powerful tool for the identification of molecular biomarkers in various medical areas, as well as for disease diagnosis or prognosis determination, analysing and identifying the potential mechanisms of various diseases, and to determine the therapeutic responses of drugs [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Serum Metabolomics Profiling of Commercially Mixed Functional Foods—Effects in Beta-Amyloid Induced Rats Measured Using 1H NMR Spectroscopy

et al. 2020

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Functional foods such as pomegranate, dates and honey were shown by various previous studies to individually have a neuroprotective effect, especially in neurodegenerative disease such as Alzheimer’s disease (AD). In this novel and original study, an 1H NMR spectroscopy tool was used to identify the metabolic neuroprotective mechanism of commercially mixed functional foods (MFF) consisting of pomegranate, dates and honey, in rats injected with amyloid-beta 1-42 (Aβ-42). Forty-five male albino Wistar rats were randomly divided into five groups: NC (0.9% normal saline treatment + phosphate buffer solution (PBS) solution injection), Abeta (0.9% normal saline treatment + 0.2 µg/µL Aβ-42 injection), MFF (4 mL/kg MFF treatment + PBS solution injection), Abeta–MFF (4 mL/kg MFF treatment + 0.2 µg/µL Aβ-42 injection) and Abeta–NAC (150 mg/kg N-acetylcysteine + 0.2 µg/µL Aβ-42 injection). Based on the results, the MFF and NAC treatment improved the spatial memory and learning using Y-maze. In the metabolic analysis, a total of 12 metabolites were identified, for which levels changed significantly among the treatment groups. Systematic metabolic pathway analysis found that the MFF and NAC treatments provided a neuroprotective effect in Aβ-42 injected rats by improving the acid amino and energy metabolisms. Overall, this finding showed that MFF might serve as a potential neuroprotective functional food for the prevention of AD.

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“…For deciding between discriminatory and non-discriminatory bins, a VIP score cut-off of 1 was used. Discriminatory signals were attribute to metabolites using HMDB platform and also based on the literature [ 23 – 26 ].…”

Section: Methodsmentioning

confidence: 99%

Diagnosis of coinfection by schistosomiasis and viral hepatitis B or C using 1H NMR-based metabonomics

Gouveia¹,

Santos²,

Silva³

et al. 2017

PLoS ONE

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BackgroundDiagnosis of liver involvement due to schistosomiasis in asymptomatic patients from endemic areas previously diagnosed with chronic hepatitis B (HBV) or C (HCV) and periportal fibrosis is challenging. H-1 Nuclear Magnetic Resonance (NMR)-based metabonomics strategy is a powerful tool for providing a profile of endogenous metabolites of low molecular weight in biofluids in a non-invasive way. The aim of this study was to diagnose periportal fibrosis due to schistosomiasis mansoni in patients with chronic HBV or HCV infection through NMR-based metabonomics models.Methodology/Principal findingsThe study included 40 patients divided into two groups: (i) 18 coinfected patients with schistosomiasis mansoni and HBV or HCV; and (ii) 22 HBV or HCV monoinfected patients. The serum samples were analyzed through H-1 NMR spectroscopy and the models were based on Principal Component Analysis (PCA) and Partial Least Squares—Discriminant Analysis (PLS-DA). Ultrasonography examination was used to ascertain the diagnosis of periportal fibrosis. Exploratory analysis showed a clear separation between coinfected and monoinfected samples. The supervised model built from PLS-DA showed accuracy, R2 and Q2 values equal to 100%, 98.1% and 97.5%, respectively. According to the variable importance in the projection plot, lactate serum levels were higher in the coinfected group, while the signals attributed to HDL serum cholesterol were more intense in the monoinfected group.Conclusions/SignificanceThe metabonomics models constructed in this study are promising as an alternative tool for diagnosis of periportal fibrosis by schistosomiasis in patients with chronic HBV or HCV infection from endemic areas for Schistosoma mansoni.

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1HNMR-based metabolomic profile of rats with experimental acute pancreatitis

Cited by 15 publications

References 31 publications

1H NMR-based metabolomic study of metabolic profiling for pollinosis

1H NMR-based metabolomic study of metabolic profiling for pollinosis

Serum Metabolomics Profiling of Commercially Mixed Functional Foods—Effects in Beta-Amyloid Induced Rats Measured Using 1H NMR Spectroscopy

Diagnosis of coinfection by schistosomiasis and viral hepatitis B or C using 1H NMR-based metabonomics

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