2002
DOI: 10.1074/jbc.m110398200
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1α,25(OH)2D3 Regulates Chondrocyte Matrix Vesicle Protein Kinase C (PKC) Directly via G-protein-dependent Mechanisms and Indirectly via Incorporation of PKC during Matrix Vesicle Biogenesis

Abstract: Matrix vesicles are extracellular organelles involved in mineral formation that are regulated by 1␣,25 (OH) 2 D 3 . Prior studies have shown that protein kinase C (PKC) activity is involved in mediating the effects of 1␣,25(OH) 2 D 3 in both matrix vesicles and plasma membranes. Here, we examined the regulation of matrix vesicle PKC by 1␣,25(OH) 2 D 3 during biogenesis and after deposition in the matrix. When growth zone costochondral chondrocytes were treated for 9 min with 1␣,25 (OH) 2 D 3 , PKC in matrix ve… Show more

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Cited by 43 publications
(30 citation statements)
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“…Similar observations have been made in vitamin D responsive tissues, where membrane initiated signaling have been linked to PKA, PKC and MAPK signaling pathways (Schwartz et al, 2002;Dirks-Naylor and Lennon-Edwards, 2011). This study was aimed to further elucidate membrane initiated signaling by 1α,25(OH) 2 D 3 in LNCaP prostate cancer cells by evaluating the effects of 1α,25(OH) 2 D 3 on AP1 and CREB-dependent gene expression as well as testing the hypothesis that 1α,25(OH) 2 D 3 might evoke membrane initiated effects through TNF-α and TNF-α initiated signaling pathways.…”
Section: Resultssupporting
confidence: 56%
See 1 more Smart Citation
“…Similar observations have been made in vitamin D responsive tissues, where membrane initiated signaling have been linked to PKA, PKC and MAPK signaling pathways (Schwartz et al, 2002;Dirks-Naylor and Lennon-Edwards, 2011). This study was aimed to further elucidate membrane initiated signaling by 1α,25(OH) 2 D 3 in LNCaP prostate cancer cells by evaluating the effects of 1α,25(OH) 2 D 3 on AP1 and CREB-dependent gene expression as well as testing the hypothesis that 1α,25(OH) 2 D 3 might evoke membrane initiated effects through TNF-α and TNF-α initiated signaling pathways.…”
Section: Resultssupporting
confidence: 56%
“…There are no direct evidences that vitamin D receptors are coupled to a protein complex which includes adenylate cyclase. However, several studies have demonstrated that 1 ,25(OH) 2 D 3 evoke rapid increases in PKA activity, intracellular cAMP concentrations which have been found to be associated with G-protein-coupled signaling as well as regulation of Ca 2+ transport through Ca 2+ -channels (Massheimer et al, 1999;Schwartz et al, 2002;Dirks-Naylor & Lennon-Edwards, 2011).…”
Section: Introductionmentioning
confidence: 99%
“…Transfection of ROS 24/1 rat osteoblast-like cells, which lack a functional 1,25-nVDR, with annexin II restores transcaltachia in response to 1a,25(OH) 2 D 3 [Baran et al, 2000b]. Moreover, we have shown that when extracellular matrix vesicles are incubated directly with 1a,25(OH) 2 D 3 , PKC activity is regulated although no 1,25-nVDR is present and there is no possibility of gene transcription or protein synthesis [Schwartz et al, 2001a]. This is the first demonstration, however, that PKC is regulated by 1a,25(OH) 2 D 3 in intact chondrocytes that lack a functional 1,25-nVDR.…”
Section: Discussionmentioning
confidence: 78%
“…24R,25(OH) 2 D 3 stimulates PKCα in resting zone chondrocytes via a phospholipase D (PLD) dependent pathway which does not involve ERp60 [23]. This also results in production of DAG, but via a two-step process, and peak activation occurs later than is seen in 1α,25 (OH) 2 PKC activity in the extracellular organelles is increased due to the specific incorporation of PKC-zeta during production [24]. PKCζ is an atypical form of PKC, requiring neither lipid not Ca ++ as co-factors.…”
Section: Mechanism Of 1α25(oh) 2 D 3 Action In the Extracellular Matrixmentioning
confidence: 98%