2,3,5,4'‑Tetrahydroxystilbene‑2‑O‑β‑D‑glucoside inhibits septic serum‑induced inflammatory injury via interfering with the ROS‑MAPK‑NF‑κB signaling pathway in pulmonary aortic endothelial cells

Abstract

Sepsis is characterized by injury to the microvasculature and the microvascular endothelial cells, leading to barrier dysfunction. However, the specific role of injury in septic endothelial barrier dysfunction remains to be elucidated. In the present study, it was hypothesized that endothelial cell inflammatory injury is likely required for barrier dysfunction under septic conditions in vitro. 2,3,5,4'‑Tetrahydroxystilbene‑2‑O‑β‑D‑glucoside (TSG), a compound extracted from Chinese herbs, is able to inhibit the inflammatory injury of septic‑serum in endothelial cells. In the present study, cell viability was assayed by CCK‑8 method; mRNA and protein expression was identified by RT‑qPCR, western blot or Elisa, respectively and the production of reactive oxygen species was observed by a fluorescence microscope. The present study indicated that septic serum significantly decreased the cell viability of pulmonary aortic endothelial cells (PAECs) following co‑cultivation for 6 h, which occurred in a time‑dependent manner. TSG notably increased the viability of PAECs in a time‑ and concentration‑dependent manner. Further investigations revealed that septic serum increased the secretion of interleukin (IL)‑1β, IL‑6 and C‑reactive protein in PAECs, whereas pretreatment with TSG significantly decreased the secretion of these inflammatory factors. These data indicated that septic serum increased inflammatory injury to the PAECs, and TSG decreased this injury via the reactive oxygen species‑mitogen‑activated protein kinase‑nuclear factor‑κB signaling pathway.

The Protective Effects of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-d-Glucoside in the OVA-Induced Asthma Mice Model

“…From this viewpoint, asthma treatment using PM extract has the potential to cause various side effects. Nevertheless, PM has various biological effects [13,14,15,16] and 2,3,5,4′-tetrahydroxystilbene-2-O-β- d -glucoside (TSG) as a main single compound has anti-inflammatory effects [14] and anti-osteoporosis effect [13]. In the present study, we investigated the effects of TSG on asthma as one of the inflammatory diseases.…”

“…Pharmacological studies have emphasized the benefits of PM in the treatment of a variety of diseases, such as cancer, diabetes, alopecia, atherosclerosis, and neurodegenerative diseases [11]. 2,3,5,4′-tetrahydroxystilbene-2-O-β- d -glucoside (TSG) is known as a major active single compound of PM [12], and presents a variety of biological effects, such as anti-osteoporosis [13], anti-inflammation [14], kidney protection [15], anti-cancer [16], and liver protection effects [17]. However, Lei et al reported that PM extracts have hepatotoxicity [18], and this is probably due to the cis isomer of TSG [19].…”

2,3,5,4’-Tetrahydroxystilbene-2-O-β-D-Glucoside modulated human umbilical vein endothelial cells injury under oxidative stress

“…Neuroprotective effects of TSG against glutamate or H 2 O 2 -induced oxidative toxicity were confirmed between cell lines or rodent models [ 7 , 8 ]. Li et al [ 9 ] confirmed that TSG decreased pulmonary aortic endothelial cell inflammatory injury induce by septic-serum via the ROS/MAPK/NF-κB signaling pathway. However, the protective effect of TSG on H 2 O 2 -induced oxidative damage remains to be fully elucidated.…”