2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) attenuates human platelet aggregation, secretion and spreading in vitro

Ke, Xiang; Li, Gang; Zhou, Yajun; Hao, Hong-Zhen; Yin, Zhang; He, Ao-Di; Da, Xing-Wen; Xiang, Jizhou; Wang, Jialing; Ming, Zhang-Yin

doi:10.1016/j.thromres.2013.11.006

Cited by 33 publications

(23 citation statements)

References 42 publications

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“…Another study indicated that TSG exerted potent anti-platelet activity against collageninduced aggregation. TSG is likely to exert protective effects in platelet-associated thromboembolic disorders by modulating human platelets (Xiang et al, 2014). Importantly, emodin (IC 50 was 200 μM) seriously blocks both the binding of SARS-CoV S protein to angiotensin-converting enzyme 2 (ACE2) and the infectivity of S protein-pseudotyped retrovirus to Vero E6 cells.…”

Section: Other Pharmacological Effectsmentioning

confidence: 99%

Traditional usages, botany, phytochemistry, pharmacology and toxicology of Polygonum multiflorum Thunb.: A review

Lin

et al. 2015

Journal of Ethnopharmacology

320

207

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Therapeutic potential of Polygonum multiflorum has been demonstrated in the conditions like Alzheimer׳s disease, Parkinson׳s disease, hyperlipidaemia, inflammation and cancer, which is attributed to the presence of various stilbenes, quinones, flavonoids, phospholipids and other compounds in the drug. On the other hand, the adverse effects (hepatotoxicity, nephrotoxicity, and embryonic toxicity) of this plant were caused by the quinones, such as emodin and rhein. Thus more pharmacological and toxicological mechanisms on main active compounds are necessary to be explored, especially the combined anthraquinones (Emodin-8-O-β-d-glucopyranoside, Physcion-8-O-β-d-glucopyranoside, etc.) and the variety of stilbenes.

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Section: Other Pharmacological Effectsmentioning

confidence: 99%

Traditional usages, botany, phytochemistry, pharmacology and toxicology of Polygonum multiflorum Thunb.: A review

Lin

et al. 2015

Journal of Ethnopharmacology

320

207

View full text Add to dashboard Cite

show abstract

“…TSG is one of the major bioactive constituents extracted from Polygonum multiflorum Thunb and demonstrates various pharmacologic activities including antioxidant, anti‐inflammatory, and anti‐atherosclerotic effects , improvement of memory and learning ability , neuroprotection , anti‐aging , promotion of hair growth , and attenuation of human platelet aggregation . It was recently reported that TSG could protect human umbilical vein endothelial cells (HUVECs) from lysophosphatidylcholine (LPC)‐induced injury by attenuating the expression of asymmetric dimethylarginine (ADMA) and enhancing the production of nitric oxide (NO) ; however, the underlying molecular mechanisms are largely unknown.…”

Section: Introductionmentioning

confidence: 99%

2,3,5,4′‐tetrahydroxystilbene‐2‐O‐β‐d‐glucoside protects human umbilical vein endothelial cells against lysophosphatidylcholine‐induced apoptosis by upregulating superoxide dismutase and glutathione peroxidase

Zhao

Fan

et al. 2014

IUBMB Life

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2,3,5,4 0 -Tetrahydroxystilbene-2-O-b-D-glucoside (TSG) has been shown to protect human umbilical vein endothelial cells (HUVECs) from lysophosphatidylcholine (LPC)-induced injury; however, the underlying molecular mechanism remains to be determined. The aim of this study was to investigate the protective mechanism of TSG against LPC-induced injury in HUVECs. We established a stable LPC-induced cell model by treating HUVECs with various concentrations of LPC and found 10.0 mg/mL of LPC to be optimal for inducing HUVECs injury. The effects of TSG on LPC-induced cell injury were assessed by cell counting kit-8, apoptosis assay, transmission electron microscope, and measurement of malondialdehyde (MDA), the antioxidant enzymes superoxide dismutase (SOD), reactive oxygen species (ROS), glutathione peroxidase, and mitochondrial membrane potential. The mRNA and protein levels of caspase-3, Bax, Bcl-2, PARP-1, and cytochrome C were assayed by real-time reverse transcriptasepolymerase chain reaction and immunoblotting, respectively. TSG pretreatment was able to prevent LPC-induced HUVECs injury and restore cell viability in a concentration-dependent manner. LPC treated cells showed typical apoptotic morphological changes including cytoplasmic vacuolation, swollen mitochondria, and characteristic biochemical hallmarks of apoptosis including loss of mitochondrial membrane potential, activation of caspase-3, decrease of Bcl-2, increase of PARP-1, upregulation of Bax, and release of cytochrome C, all of which were apparently inhibited by TSG pretreatment. Treatment of HUVECs with LPC led to decrease of SOD and glutathione peroxidase in addition to rapid increase of MDA and ROS levels. Pretreatment with TSG restored SOD and glutathione peroxidase levels to that of normal levels, and significantly decreased ROS and MDA levels. Our data indicate that TSG inhibits apoptosis of HUVECs mediated by LPC through blocking the mitochondrial apoptotic pathway and suggest that the mechanisms underlying the protective effects of TSG are related to the activation of SOD and glutathione peroxidase, the clearance of intracellular ROS, and reduction of lipid peroxidation.

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“…The lipid regulation activity might be due to an overall synergy of TSG, emodin, and physcion. TSG attenuates human platelet aggregation, secretion, and spreading in vitro (Xiang et al, 2014).…”

Section: Effects On Cardiovascular Systemmentioning

confidence: 95%

Phytochemical and biological research of Polygoneae medicinal resources

Hao¹,

Gu²,

Xiao³

2015

Medicinal Plants

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2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) attenuates human platelet aggregation, secretion and spreading in vitro

Cited by 33 publications

References 42 publications

Traditional usages, botany, phytochemistry, pharmacology and toxicology of Polygonum multiflorum Thunb.: A review

Traditional usages, botany, phytochemistry, pharmacology and toxicology of Polygonum multiflorum Thunb.: A review

2,3,5,4′‐tetrahydroxystilbene‐2‐O‐β‐d‐glucoside protects human umbilical vein endothelial cells against lysophosphatidylcholine‐induced apoptosis by upregulating superoxide dismutase and glutathione peroxidase

Phytochemical and biological research of Polygoneae medicinal resources

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