2,3‐Butanediol catabolism in <i>Pseudomonas aeruginosa</i> PAO1

Liu, Qiuyuan; Liu, Yidong; Kang, Zhaoqi; Xiao, Dan; Gao, Chao; Xu, Ping; Ma, Cuiqing

doi:10.1111/1462-2920.14332

Cited by 23 publications

(18 citation statements)

References 48 publications

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“…Likely, the answer lies in the lifestyle carried out by the microorganism at this condition. In this context, and as mentioned before, it has been shown that utilization of BD, a common fermentation product of P. aeruginosa co-habitant bacteria, significantly increases virulence and infection of the microorganism (Venkataraman et al, 2014; Nguyen et al, 2016; Liu et al, 2018). The activation of the pathway of BD utilization through acetoin by light observed could plausibly go in this same sense too in A. baumannii .…”

Section: Discussionmentioning

confidence: 54%

Quorum and Light Signals Modulate Acetoin/Butanediol Catabolism in Acinetobacter spp.

et al. 2019

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Acinetobacter spp. are found in all environments on Earth due to their extraordinary capacity to survive in the presence of physical and chemical stressors. In this study, we analyzed global gene expression in airborne Acinetobacter sp. strain 5-2Ac02 isolated from hospital environment in response to quorum network modulators and found that they induced the expression of genes of the acetoin/butanediol catabolism, volatile compounds shown to mediate interkingdom interactions. Interestingly, the acoN gene, annotated as a putative transcriptional regulator, was truncated in the downstream regulatory region of the induced acetoin/butanediol cluster in Acinetobacter sp. strain 5-2Ac02, and its functioning as a negative regulator of this cluster integrating quorum signals was confirmed in Acinetobacter baumannii ATCC 17978. Moreover, we show that the acetoin catabolism is also induced by light and provide insights into the light transduction mechanism by showing that the photoreceptor BlsA interacts with and antagonizes the functioning of AcoN in A. baumannii , integrating also a temperature signal. The data support a model in which BlsA interacts with and likely sequesters AcoN at this condition, relieving acetoin catabolic genes from repression, and leading to better growth under blue light. This photoregulation depends on temperature, occurring at 23°C but not at 30°C. BlsA is thus a dual regulator, modulating different transcriptional regulators in the dark but also under blue light, representing thus a novel concept. The overall data show that quorum modulators as well as light regulate the acetoin catabolic cluster, providing a better understanding of environmental as well as clinical bacteria.

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Section: Discussionmentioning

confidence: 54%

Quorum and Light Signals Modulate Acetoin/Butanediol Catabolism in Acinetobacter spp.

et al. 2019

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“…The effector of the 2,3-butanediol operon in P. aeruginosa PAO1 is the metabolic intermediate acetaldehyde (41). Although the effector promiscuity of CsiR may be due to the structural similarity of l -2-HG and glutarate, it is also possible that the response of CsiR to l- 2-HG has a physiological significance.…”

Section: Discussionmentioning

confidence: 99%

Regulation of Glutarate Catabolism by GntR Family Regulator CsiR and LysR Family Regulator GcdR in Pseudomonas putida KT2440

Zhang

Kang

Guo

et al. 2019

mBio

Self Cite

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Glutarate, a metabolic intermediate in the catabolism of several amino acids and aromatic compounds, can be catabolized through both the glutarate hydroxylation pathway and the glutaryl-coenzyme A (glutaryl-CoA) dehydrogenation pathway in Pseudomonas putida KT2440. The elucidation of the regulatory mechanism could greatly aid in the design of biotechnological alternatives for glutarate production. In this study, it was found that a GntR family protein, CsiR, and a LysR family protein, GcdR, regulate the catabolism of glutarate by repressing the transcription of csiD and lhgO, two key genes in the glutarate hydroxylation pathway, and by activating the transcription of gcdH and gcoT, two key genes in the glutaryl-CoA dehydrogenation pathway, respectively. Our data suggest that CsiR and GcdR are independent and that there is no cross-regulation between the two pathways. l-2-Hydroxyglutarate (l-2-HG), a metabolic intermediate in the glutarate catabolism with various physiological functions, has never been elucidated in terms of its metabolic regulation. Here, we reveal that two molecules, glutarate and l-2-HG, act as effectors of CsiR and that P. putida KT2440 uses CsiR to sense glutarate and l-2-HG and to utilize them effectively. This report broadens our understanding of the bacterial regulatory mechanisms of glutarate and l-2-HG catabolism and may help to identify regulators of l-2-HG catabolism in other species. IMPORTANCE Glutarate is an attractive dicarboxylate with various applications. Clarification of the regulatory mechanism of glutarate catabolism could help to block the glutarate catabolic pathways, thereby improving glutarate production through biotechnological routes. Glutarate is a toxic metabolite in humans, and its accumulation leads to a hereditary metabolic disorder, glutaric aciduria type I. The elucidation of the functions of CsiR and GcdR as regulators that respond to glutarate could help in the design of glutarate biosensors for the rapid detection of glutarate in patients with glutaric aciduria type I. In addition, CsiR was identified as a regulator that also regulates l-2-HG metabolism. The identification of CsiR as a regulator that responds to l-2-HG could help in the discovery and investigation of other regulatory proteins involved in l-2-HG catabolism.

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“…Moreover, genes involved in the central carbon catabolism and energy metabolism were identified among the upregulated genes in the core biofilm transcriptome. We found acoABCX, involved in the conversion of 2,3-butanediol into aldehydes, 44 to be highly expressed under biofilm conditions as well as the ethanol oxidation genes (exaABDE), which catalyze the conversion of ethanol via acetaldehyde to acetate and subsequently to acetyl-CoA before its introduction into the glyoxylate cycle for further metabolism. 44 Among the downregulated genes were genes involved in the denitrification pathway including genes of the nir, nor, and nos operons.…”

Section: Biofilm Phenotypes Of Clinical P Aeruginosa Isolates Fall Imentioning

confidence: 97%

“…We found acoABCX, involved in the conversion of 2,3-butanediol into aldehydes, 44 to be highly expressed under biofilm conditions as well as the ethanol oxidation genes (exaABDE), which catalyze the conversion of ethanol via acetaldehyde to acetate and subsequently to acetyl-CoA before its introduction into the glyoxylate cycle for further metabolism. 44 Among the downregulated genes were genes involved in the denitrification pathway including genes of the nir, nor, and nos operons. Furthermore, genes of the arginine succinyltransferase (AST) pathway (aruBG encoding the AST; astD and argD) required for aerobic arginine catabolism were commonly downregulated.…”

Section: Biofilm Phenotypes Of Clinical P Aeruginosa Isolates Fall Imentioning

confidence: 97%

Parallel evolutionary paths to produce more than one Pseudomonas aeruginosa biofilm phenotype

Thöming

Tomasch

Preuße

et al. 2020

npj Biofilms Microbiomes

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Studying parallel evolution of similar traits in independent within-species lineages provides an opportunity to address evolutionary predictability of molecular changes underlying adaptation. In this study, we monitored biofilm forming capabilities, motility, and virulence phenotypes of a plethora of phylogenetically diverse clinical isolates of the opportunistic pathogen Pseudomonas aeruginosa. We also recorded biofilm-specific and planktonic transcriptional responses. We found that P. aeruginosa isolates could be stratified based on the production of distinct organismal traits. Three major biofilm phenotypes, which shared motility and virulence phenotypes, were produced repeatedly in several isolates, indicating that the phenotypes evolved via parallel or convergent evolution. Of note, while we found a restricted general response to the biofilm environment, the individual groups of biofilm phenotypes reproduced biofilm transcriptional profiles that included the expression of well-known biofilm features, such as surface adhesive structures and extracellular matrix components. Our results provide insights into distinct ways to make a biofilm and indicate that genetic adaptations can modulate multiple pathways for biofilm development that are followed by several independent clinical isolates. Uncovering core regulatory pathways that drive biofilm-associated growth and tolerance towards environmental stressors promises to give clues to host and environmental interactions and could provide useful targets for new clinical interventions.

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2,3‐Butanediol catabolism in Pseudomonas aeruginosa PAO1

Cited by 23 publications

References 48 publications

Quorum and Light Signals Modulate Acetoin/Butanediol Catabolism in Acinetobacter spp.

Quorum and Light Signals Modulate Acetoin/Butanediol Catabolism in Acinetobacter spp.

Regulation of Glutarate Catabolism by GntR Family Regulator CsiR and LysR Family Regulator GcdR in Pseudomonas putida KT2440

Parallel evolutionary paths to produce more than one Pseudomonas aeruginosa biofilm phenotype

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