2,3-Butanediol production by Enterobacter aerogenes: selection of the optimal conditions and application to food industry residues

Perego, Patrizia; Converti, Attílio; Borghi, Adriana Del; Canepa, Paolo

doi:10.1007/s004490000210

Cited by 71 publications

(48 citation statements)

References 11 publications

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“…Based on the obtained experimental data, six factors having significant influence on the 2,3-butanediol production were selected for the present Taguchi DOE study to optimize the submerged culture condition. Six factors (temperature, pH, agitation, inoculum's size, acetic acid and succinic acid) which showed significantly influence on the 2,3-butanediol production (Perego et al, 2000;Ghosh and Swaminathan, 2003;Perego et al, 2003) were considered in the present experimental situation ( Table 1).…”

Section: Taguchi Methodologymentioning

confidence: 99%

Enhancement of 2,3-butanediol production by Klebsiella pneumoniae PTCC 1290: Application of Taguchi methodology for process optimization

Khayati¹,

Pahlevanzadeh²,

Ghaemi³

et al. 2009

Afr. J. Biotechnol.

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2,3-Butanediol production parameter optimization using Klebsiella pneumoniae PTCC 1290 was performed using the design of experiments available in the form of an orthogonal array and a software for automatic design and analysis of the experiments, both based on Taguchi protocol. Optimal levels of physical parameters and key media components namely temperature, pH, inoculum size, agitation, acetic acid and succinic acid were determined. 2,3-Butanediol production obtained from the 18 sets of fermentation experiments performed with the selected factors and levels were further processed with Qualitek-4 software at bigger is better as quality character. The optimized conditions showed an enhanced 2,3-butanediol production of 35.8% (from 11.856 to 18.459 g l -1). The optimal combinations of factors obtained from the proposed design of experiments methodology was further validated by conducting fermentation experiments and the obtained results revealed an enhanced 2,3-butanediol production of 25.8%. Taguchi approach of design of experiments resulted in evaluating the main and interaction effects of the factors individually and in combination.

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Section: Taguchi Methodologymentioning

confidence: 99%

Enhancement of 2,3-butanediol production by Klebsiella pneumoniae PTCC 1290: Application of Taguchi methodology for process optimization

Khayati¹,

Pahlevanzadeh²,

Ghaemi³

et al. 2009

Afr. J. Biotechnol.

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“…Bacterial 2,3-BDO production has been shown e.g. with strains of Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter aerogenes, Bacillus polymyxa, and Bacillus licheniformis (Grover et al, 1990, Perego et al, 2000, De Mas et al, 1988, Nilegaonkar et al, 1996, Jansen et al, 1984. Biosynthesis of 2,3-BDO is funneled from pyruvate in three steps.…”

Section: 3-butanediolmentioning

confidence: 99%

“…With B. licheniformis corn starch hydrolysates were applied to 2,3-BDO production (Perego et al, 2003). With E. aerogenes, food industry wastes such as starch hydrolysates, raw and decoloured molasses, and whey permeate were used for the fermentation of 2,3-BDO (Perego et al, 2000). The use of lignocellulosic compounds for 2,3-BDO has also been reported, e.g.…”

Section: 3-butanediolmentioning

confidence: 99%

Use of Glycerol in Biotechnological Applications

Wendisch¹,

Lindner²,

Meiswinkel³

2011

Biodiesel- Quality, Emissions and by-Products

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“…Os resíduos da indústria alimentícia constituem uma importante fração dos substratos que podem ser utilizados para a produção de 2,3-butanodiol, uma vez que alguns desses resíduos apresentam altos teores de açúcares (PEREGO et al, 2000;.…”

Section: Resíduos Da Indústria Alimentíciaunclassified

“…Além da diferença da composição química, a concentração de lactose no soro de leite é baixa, sendo aproximadamente 4,0% da massa seca (PEREGO et al, 2000).…”

Section: Resíduos Da Indústria Alimentíciaunclassified

Produção de celulases por fungos de ambiente marinho e terrestre para uso na hidrólise do bagaço de cana-de-açúcar e produção de 2,3-butanodiol pela bactéria <i>Serratia marcescens</i> a partir de glicose e glicerol

Santos¹

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In Chapter 1 it is reported the cellulase production by 4 marine-derived fungi strains (Aspergillus sydowii CBMAI 934, A. sydowii CBMAI 935, Penicillium citrinum CBMAI 1186 and Mucor racemosus CBMAI 847) and 1 terrestrial fungi strain (Aspergillus sp. CBMAI 1198). They were grown in solid state fermentation using wheat straw as substrate (5 g) and with addition of peptone solution (0,75 g.L -1 ) enriched with inorganic salts. It was performed the enhancement of the growth conditions by changing the temperature, initial pH and moisture. The optimum temperature for all strains varied between 25-35 °C but A. sydowii CBMAI 935 with 40 °C. The optimum pH was different for each strain, varying from acidic to alkaline conditions. The optimum moisture content also varied accordingly the studied strain. In order enhance the cellulose hydrolysis performed by the produced cellulases, it was varied the pH, temperature and amount of the crude cellulase extract during the filter paper hydrolysis reaction. The obtained optimum values were different among strains and, in some cases, there were two optimum pH and temperature for the hydrolysis of the filter paper. Then, the obtained cellulases, using the best conditions for hydrolysis, were used in the sugarcane bagasse hydrolysis (10 FPU/g of sugarcane bagasse). The cellulases from the strains Aspergillus sp. CBMAI 1198 and A. sydowii CBMAI 934 were capable of degrading 75% and 78% of the sugarcane bagasse, respectively, generating reducing sugars. In Chapter 2, the capability of 6 strains (Bacillus subtilis LQOB-SE1, B. coagulans LQOB-SE2, B. pumillus LQOB-SE3, Brevibacillus brevis LQOB-SE4, Lysinibacillus sp. LQOB-SE5 and Serratia marcescens LQOB-SE6), isolated from peat soil, of producing 2,3-butanediol from glycerol fermentation. The only strain that produced 2,3-butanediol was S. marcescens LQOB-SE6, which was also applied in 2,3-butanediol production from glucose fermentation. Therefore, wastes from biodiesel and bioethanol production can be reused in industrial scale. The best conditions for glycerol fermentation: initial pH 7, Nutrient Broth (8 g. Tabela 3. Rendimento e produtividade obtidos de 2,3-butanodiol a partir da fermentação do glicerol pela bactéria S. marcescens LQOB-SE6 ao longo do tempo de cultivo. 182Tabela 4. Comparação entre os valores de rendimentos e produtividades obtidos na produção de 2,3-butanodiol a partir da fermentação do glicerol com resultados descritos na literatura para diferentes microrganismos. 183Tabela 5. Rendimentos e produtividades obtidos a partir da fermentação da glicose pela bactéria S. marcescens LQOB-SE6 ao longo do tempo de cultivo. 191Tabela 6. Dados de rendimentos e produtividades obtidos na produção de 2,3-butanodiol a partir da fermentação da glicose com microrganismos relatados na literatura e com a bactéria S. marcescens LQOB-SE6.

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2,3-Butanediol production by Enterobacter aerogenes: selection of the optimal conditions and application to food industry residues

Abstract: Optimum values of temperature, pH and starting substrate concentration were determined for 2,3-butanediol production by Enterobacter aerogenes

Cited by 71 publications

References 11 publications

Enhancement of 2,3-butanediol production by Klebsiella pneumoniae PTCC 1290: Application of Taguchi methodology for process optimization

Enhancement of 2,3-butanediol production by Klebsiella pneumoniae PTCC 1290: Application of Taguchi methodology for process optimization

Use of Glycerol in Biotechnological Applications

Produção de celulases por fungos de ambiente marinho e terrestre para uso na hidrólise do bagaço de cana-de-açúcar e produção de 2,3-butanodiol pela bactéria <i>Serratia marcescens</i> a partir de glicose e glicerol

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