2,3 Butanediol production in an obligate photoautotrophic cyanobacterium in dark conditions via diverse sugar consumption

McEwen, Jordan T.; Kanno, Masahiro; Atsumi, Shota

doi:10.1016/j.ymben.2016.03.004

Cited by 42 publications

(37 citation statements)

References 42 publications

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“…That the yield is above 100% indicates that both CO 2 and glucose were successfully utilized with at least 36% of carbons derived from CO 2 . Given that the yield of 23BD in photomixotrophic conditions reported in our previous study was only 40% of the TMY13, the efficiency of production achieved in this study represents a dramatic improvement.…”

Section: Resultsmentioning

confidence: 60%

“…However, in order to achieve industrial feasibility, chemical production under both light and dark conditions is essential. As demonstrated in our previous work, concurrent expression of heterologous sugar importers and the 23BD biosynthetic pathway genes allows for chemical production and growth from both CO 2 and glucose13. The oxidation of sugars provides an increased supply of metabolites and energy independent of photosynthesis, allowing for faster cell growth and continuous chemical production throughout diurnal conditions (12 h light/12 h dark).…”

mentioning

confidence: 78%

“…We have previously engineered the model cyanobacterium Synechococcus elongatus PCC 7942 to produce 2,3-butanediol (23BD) from CO 2 and glucose111213. Under natural light, chemical production from an engineered photosynthetic organism would be confined to a limited window of optimal sunlight exposure13.…”

mentioning

confidence: 99%

“…Under natural light, chemical production from an engineered photosynthetic organism would be confined to a limited window of optimal sunlight exposure13. However, in order to achieve industrial feasibility, chemical production under both light and dark conditions is essential.…”

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confidence: 99%

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Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria

2017

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Cyanobacteria have attracted much attention as hosts to recycle CO2 into valuable chemicals. Although cyanobacteria have been engineered to produce various compounds, production efficiencies are too low for commercialization. Here we engineer the carbon metabolism of Synechococcus elongatus PCC 7942 to improve glucose utilization, enhance CO2 fixation and increase chemical production. We introduce modifications in glycolytic pathways and the Calvin Benson cycle to increase carbon flux and redirect it towards carbon fixation. The engineered strain efficiently uses both CO2 and glucose, and produces 12.6 g l−1 of 2,3-butanediol with a rate of 1.1 g l−1 d−1 under continuous light conditions. Removal of native regulation enables carbon fixation and 2,3-butanediol production in the absence of light. This represents a significant step towards industrial viability and an excellent example of carbon metabolism plasticity.

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Section: Resultsmentioning

confidence: 60%

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confidence: 78%

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confidence: 99%

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confidence: 99%

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Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria

2017

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“…Recently, the model cyanobacterium Synechococcus 7942 has been engineered to utilize organic carbon sources, including glucose, xylose, and glycerol, to grow continuously under diurnal conditions. These engineered mixotrophic strains exhibited better performance in chemical production .…”

Section: Expression Of Heterologous Genesmentioning

confidence: 99%

Synthetic Biology Toolkits for Metabolic Engineering of Cyanobacteria

Xia

Ling

Foo

et al. 2019

Biotechnology Journal

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Cyanobacteria are of great importance to Earth's ecology. Due to their capability in photosynthesis and C1 metabolism, they are ideal microbial chassis that can be engineered for direct conversion of carbon dioxide and solar energy into biofuels and biochemicals. Facilitated by the elucidation of the basic biology of the photoautotrophic microbes and rapid advances in synthetic biology, genetic toolkits have been developed to enable implementation of nonnatural functionalities in engineered cyanobacteria. Hence, cyanobacteria are fast becoming an emerging platform in synthetic biology and metabolic engineering. Herein, the progress made in the synthetic biology toolkits for cyanobacteria and their utilization for transforming cyanobacteria into microbial cell factories for sustainable production of biofuels and biochemicals is outlined. Current techniques in heterologous gene expression, strategies in genome editing, and development of programmable regulatory parts and modules for engineering cyanobacteria towards biochemical production are discussed and prospected. As cyanobacteria synthetic biology is still in its infancy, apart from the achievements made, the difficulties and challenges in applying and developing genetic toolkits in cyanobacteria for biochemical production are also evaluated.

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Growth engineering of Synechococcus elongatus PCC 7942 for mixotrophy under natural light conditions for improved feedstock production

Sarnaik¹,

Pandit²,

Lali

2017

Biotechnology Progress

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Synechococcus elongatus PCC 7942 has been widely explored as cyanobacterial cell factory through genetic modifications for production of various value-added compounds. However, successful industrial scale-ups have not been reported for the system predominantly due to its obligate photoautotrophic metabolism and use of artificial light in photobioreactors. Hence, engineering the organism to perform mixotrophy under natural light could serve as an effective solution. Thus, we applied a genetically engineered strain of Synechococcus elongatus PCC 7942 expressing heterologous hexose transporter gene (galP) to perform mixotrophy under natural light in a temperature controlled environmental chamber (EC). We systematically studied the comparative performances of these transformants using autotrophy and mixotrophy, which showed 3.4 times increase in biomass productivity of mixotrophically grown transformants over autotrophs in EC. Chlorophyll a yield was found to have decreased in mixotrophic conditions, possibly indicating reduced dependency on light for energy metabolism. Although pigment yield decreases under mixotrophy, titer was found to have improved due to increased biomass productivity. Carotenoid analysis showed that zeaxanthin is the major carotenoid produced by the species which is essential for photoprotection. Our work thus demonstrates that mixotrophy under temperature controlled natural light can serve as the viable solution to improve biomass productivity of Synechococcus elongatus PCC 7942 and for commercial production of natural or engineered value added compounds from the system. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1182-1192, 2017.

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2,3 Butanediol production in an obligate photoautotrophic cyanobacterium in dark conditions via diverse sugar consumption

Cited by 42 publications

References 42 publications

Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria

Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria

Synthetic Biology Toolkits for Metabolic Engineering of Cyanobacteria

Growth engineering of Synechococcus elongatus PCC 7942 for mixotrophy under natural light conditions for improved feedstock production

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